Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   SE693_RS00690 Genome accession   NZ_CP138412
Coordinates   156431..156871 (+) Length   146 a.a.
NCBI ID   WP_000360894.1    Uniprot ID   -
Organism   Escherichia coli strain 50EVA     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 151431..161871
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SE693_RS00670 aroP 152429..153802 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  SE693_RS00675 ampE 153845..154699 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  SE693_RS00680 ampD 154696..155247 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  SE693_RS00685 nadC 155335..156228 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  SE693_RS00690 pilA 156431..156871 (+) 441 WP_000360894.1 prepilin peptidase-dependent pilin Machinery gene
  SE693_RS00695 pilB 156881..158266 (+) 1386 WP_001025155.1 type II secretion system protein GspE Machinery gene
  SE693_RS00700 hofC 158256..159458 (+) 1203 WP_000157234.1 protein transport protein HofC -
  SE693_RS00705 guaC 159493..160536 (-) 1044 WP_001217338.1 GMP reductase -
  SE693_RS00710 - 160692..160736 (-) 45 WP_120795372.1 protein YacM -
  SE693_RS00715 coaE 160761..161381 (+) 621 WP_001269527.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15611.72 Da        Isoelectric Point: 4.5538

>NTDB_id=826012 SE693_RS00690 WP_000360894.1 156431..156871(+) (pilA) [Escherichia coli strain 50EVA]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=826012 SE693_RS00690 WP_000360894.1 156431..156871(+) (pilA) [Escherichia coli strain 50EVA]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCATC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

41.791

91.781

0.384
  pilA Haemophilus influenzae Rd KW20

41.045

91.781

0.377