Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   NUG19_RS02400 Genome accession   NZ_CP123853
Coordinates   448843..449340 (+) Length   165 a.a.
NCBI ID   WP_029694050.1    Uniprot ID   -
Organism   Staphylococcus aureus strain FDA209P     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 443843..454340
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NUG19_RS02360 - 443887..444873 (+) 987 WP_000161314.1 ROK family glucokinase -
  NUG19_RS02365 - 444873..445202 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  NUG19_RS02370 - 445199..445822 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  NUG19_RS02375 comGA 445874..446848 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  NUG19_RS02380 comGB 446820..447890 (+) 1071 WP_000776421.1 competence type IV pilus assembly protein ComGB Machinery gene
  NUG19_RS02385 comGC 447904..448215 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  NUG19_RS02390 comGD 448193..448639 (+) 447 WP_072433556.1 competence type IV pilus minor pilin ComGD Machinery gene
  NUG19_RS02395 comGE 448626..448925 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  NUG19_RS02400 comGF 448843..449340 (+) 498 WP_029694050.1 competence type IV pilus minor pilin ComGF Machinery gene
  NUG19_RS02405 - 449437..449583 (+) 147 WP_001789879.1 hypothetical protein -
  NUG19_RS02410 - 449573..450097 (+) 525 WP_001015121.1 shikimate kinase -
  NUG19_RS02415 gcvT 450256..451347 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  NUG19_RS02420 gcvPA 451367..452713 (+) 1347 WP_042727693.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  NUG19_RS02425 gcvPB 452706..454178 (+) 1473 WP_000202188.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19199.92 Da        Isoelectric Point: 10.2192

>NTDB_id=822795 NUG19_RS02400 WP_029694050.1 448843..449340(+) (comGF) [Staphylococcus aureus strain FDA209P]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIELRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTSFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=822795 NUG19_RS02400 WP_029694050.1 448843..449340(+) (comGF) [Staphylococcus aureus strain FDA209P]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGAGTAGATAGAAACGATATTGAACTTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CTTCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(68-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus MW2

96.97

100

0.97
  comGF Staphylococcus aureus N315

96.97

100

0.97