Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   R5031_RS21290 Genome accession   NZ_CP137512
Coordinates   4550927..4551550 (+) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain HPA0271     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4545927..4556550
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R5031_RS21275 (R5031_21275) - 4546614..4549085 (-) 2472 WP_003095841.1 penicillin-binding protein 1A -
  R5031_RS21280 (R5031_21280) pilM 4549270..4550334 (+) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  R5031_RS21285 (R5031_21285) pilN 4550334..4550930 (+) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  R5031_RS21290 (R5031_21290) pilO 4550927..4551550 (+) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  R5031_RS21295 (R5031_21295) comL/pilP 4551547..4552071 (+) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  R5031_RS21300 (R5031_21300) pilQ 4552125..4554269 (+) 2145 WP_003114575.1 type 4a pilus secretin PilQ Machinery gene
  R5031_RS21305 (R5031_21305) aroK 4554281..4554799 (+) 519 WP_003095833.1 shikimate kinase AroK -
  R5031_RS21310 (R5031_21310) aroB 4554849..4555955 (+) 1107 WP_003114574.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=821791 R5031_RS21290 WP_003103268.1 4550927..4551550(+) (pilO) [Pseudomonas aeruginosa strain HPA0271]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=821791 R5031_RS21290 WP_003103268.1 4550927..4551550(+) (pilO) [Pseudomonas aeruginosa strain HPA0271]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGCGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401