Detailed information NTG0821115

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: QEZ34_RS20245
• Genome accession: NZ_CP123430
• Coordinates: 4136928..4137728 (+)
• Length: 266 a.a.
• NCBI ID: WP_021441214.1
• Uniprot ID: A0A4V0HF22
• Organism: Klebsiella pneumoniae strain SH22PE16
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4131928..4142728

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QEZ34_RS20210	gspH	4132084..4132599 (+)	516	WP_023282194.1	type II secretion system minor pseudopilin GspH	-
QEZ34_RS20215	gspI	4132596..4132961 (+)	366	WP_023282193.1	type II secretion system minor pseudopilin GspI	-
QEZ34_RS20220	gspJ	4132958..4133554 (+)	597	WP_004145893.1	type II secretion system minor pseudopilin GspJ	-
QEZ34_RS20225	gspK	4133551..4134537 (+)	987	WP_023282192.1	type II secretion system minor pseudopilin GspK	-
QEZ34_RS20230	gspL	4134524..4135717 (+)	1194	WP_032418425.1	type II secretion system protein GspL	-
QEZ34_RS20235	-	4135710..4136195 (+)	486	WP_023282190.1	type II secretion system protein M	-
QEZ34_RS20240	-	4136185..4136931 (+)	747	WP_023282189.1	type II secretion system protein N	-
QEZ34_RS20245	pilD	4136928..4137728 (+)	801	WP_021441214.1	A24 family peptidase	Machinery gene
QEZ34_RS20250	hrpB	4137776..4140205 (-)	2430	WP_023282188.1	ATP-dependent helicase HrpB	-
QEZ34_RS20255	thpR	4140278..4140814 (+)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
QEZ34_RS20260	sfsA	4140814..4141530 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
QEZ34_RS20265	dksA	4141693..4142148 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29419.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=821115 QEZ34_RS20245 WP_021441214.1 4136928..4137728(+) (pilD) [Klebsiella pneumoniae strain SH22PE16]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
SRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=821115 QEZ34_RS20245 WP_021441214.1 4136928..4137728(+) (pilD) [Klebsiella pneumoniae strain SH22PE16]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
AGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
TCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (123-231)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

3D structure

• AlphaFold DB: A0A4V0HF22

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425