Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   R5026_RS15225 Genome accession   NZ_CP137495
Coordinates   3163079..3163951 (-) Length   290 a.a.
NCBI ID   WP_003094651.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain HPA0875     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3158079..3168951
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R5026_RS15180 (R5026_15180) - 3158940..3159110 (-) 171 WP_003094672.1 DUF3094 family protein -
  R5026_RS15185 (R5026_15185) - 3159136..3159585 (-) 450 WP_003094670.1 MOSC domain-containing protein -
  R5026_RS15190 (R5026_15190) - 3159582..3160211 (-) 630 WP_003094668.1 DUF1780 domain-containing protein -
  R5026_RS15195 (R5026_15195) - 3160344..3160769 (+) 426 WP_003094666.1 GNAT family N-acetyltransferase -
  R5026_RS15200 (R5026_15200) - 3160766..3161236 (+) 471 WP_003094664.1 hypothetical protein -
  R5026_RS15205 (R5026_15205) - 3161233..3161922 (+) 690 WP_003094662.1 energy-coupling factor ABC transporter permease -
  R5026_RS15210 (R5026_15210) - 3162028..3162237 (+) 210 WP_003094660.1 hypothetical protein -
  R5026_RS15215 (R5026_15215) yacG 3162274..3162474 (-) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  R5026_RS15220 (R5026_15220) coaE 3162471..3163082 (-) 612 WP_003094654.1 dephospho-CoA kinase -
  R5026_RS15225 (R5026_15225) pilD 3163079..3163951 (-) 873 WP_003094651.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  R5026_RS15230 (R5026_15230) pilC 3163955..3165175 (-) 1221 WP_003094649.1 type II secretion system F family protein Machinery gene
  R5026_RS15235 (R5026_15235) pilB 3165179..3166879 (-) 1701 WP_003094647.1 type IV-A pilus assembly ATPase PilB Machinery gene
  R5026_RS15240 (R5026_15240) pilA 3167109..3167573 (+) 465 WP_031630343.1 pilin Machinery gene
  R5026_RS15250 (R5026_15250) nadC 3167777..3168625 (-) 849 WP_003094645.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 290 a.a.        Molecular weight: 31852.89 Da        Isoelectric Point: 7.7068

>NTDB_id=821078 R5026_RS15225 WP_003094651.1 3163079..3163951(-) (pilD) [Pseudomonas aeruginosa strain HPA0875]
MPLLDYLASHPLAFVLCAILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQANYNLVLPNSACPRCGHEIR
PWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide


Download         Length: 873 bp        

>NTDB_id=821078 R5026_RS15225 WP_003094651.1 3163079..3163951(-) (pilD) [Pseudomonas aeruginosa strain HPA0875]
ATGCCCCTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCGCCATCCTCCTCGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAAGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGAACTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGCCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGTCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCGAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(137-245)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-126)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

55.516

96.897

0.538
  pilD Vibrio campbellii strain DS40M4

52.65

97.586

0.514
  pilD Acinetobacter nosocomialis M2

50.53

97.586

0.493
  pilD Acinetobacter baumannii D1279779

50.53

97.586

0.493
  pilD Neisseria gonorrhoeae MS11

48.921

95.862

0.469