Detailed information    

insolico Bioinformatically predicted

Overview

Name   comA   Type   Machinery gene
Locus tag   QEP70_RS20280 Genome accession   NZ_CP123221
Coordinates   4416353..4418569 (+) Length   738 a.a.
NCBI ID   WP_031599463.1    Uniprot ID   -
Organism   Pseudomonas amygdali pv. aesculi strain 2250     
Function   ssDNA transport through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4411353..4423569
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QEP70_RS20265 - 4413692..4414315 (+) 624 WP_005733933.1 glutathione S-transferase -
  QEP70_RS20270 - 4414499..4415431 (+) 933 WP_005733932.1 ABC transporter ATP-binding protein -
  QEP70_RS20275 - 4415428..4416216 (+) 789 WP_002552673.1 ABC transporter permease -
  QEP70_RS20280 comA 4416353..4418569 (+) 2217 WP_031599463.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  QEP70_RS20285 exbB 4418612..4419328 (+) 717 WP_010214839.1 MotA/TolQ/ExbB proton channel family protein Machinery gene
  QEP70_RS20290 - 4419325..4419756 (+) 432 WP_005733929.1 biopolymer transporter ExbD -
  QEP70_RS20295 lpxK 4419756..4420751 (+) 996 WP_002552678.1 tetraacyldisaccharide 4'-kinase -
  QEP70_RS20300 - 4420769..4420954 (+) 186 WP_002552679.1 Trm112 family protein -
  QEP70_RS20305 kdsB 4420951..4421715 (+) 765 WP_005733926.1 3-deoxy-manno-octulosonate cytidylyltransferase -
  QEP70_RS20310 murB 4421750..4422769 (+) 1020 WP_005733925.1 UDP-N-acetylmuramate dehydrogenase -

Sequence

Protein


Download         Length: 738 a.a.        Molecular weight: 80512.82 Da        Isoelectric Point: 9.9505

>NTDB_id=820087 QEP70_RS20280 WP_031599463.1 4416353..4418569(+) (comA) [Pseudomonas amygdali pv. aesculi strain 2250]
MRTGMIALASGLVALRFLPALPPTWLLLLMPILALMLLPFRTYPLALFLLGFTWACVSAQWALNDRLVHRLDGQTLWLQG
KVVGLPSVAEGVVRFELEGARSRRAILPARIRVAWYGGPQVNSGERWRMAVKLKRPAGLVNPDAFDYEAWLLAQRIGATG
TVVDGQLLTPARGAWRDLIRQRLLAVDAQGREGGLAALVLGDGSGLSSTDWQVLQDTGTVHLLVISGQHIGLLAGVIYAL
VAGLARWGLWPRALPWLPWACALAFIAALGYGLLAGFEVPVRRACVMVAMVLLWRLRFRHLGVVWPLLLSFNAVLIFEPL
VTLQPGFWLSFAAVGILVLIFSGRLGAWNWWQSWTRAQWLIAIGLLPILLALNLPISLSGPFANLLAVPWVSLIVLPPAL
LGTLLLPVSGVGEGLLWLAGGALEWLFMFLGWVAAVLPAWLPSAMPGWAWLLSLLGAFILLLPKGVPMRLLGWPLLLLCV
FPPLDSVPAGQVEVLQLDVGQGLAILLRTRNHTLLYDAGPRFGEFDIGQRVVVPAMRKAGVRHLDLMLISHADSDHAGGA
AAVHQAFPVDRVLGGELARLAPQLDARLCESDARWEWDGVMFSTWRWEQGPDGNPASCMLSVDAGGERLLLTGDIDVDAE
RAAIASGFDLRAHWLQSPHHGSRTSSSKVFLQAVGAVGVLISRGRNNAFGHPHPLVMARYRWLGMASYDSAELGAVSLRL
GTFGTPQAERAQRRFWRD

Nucleotide


Download         Length: 2217 bp        

>NTDB_id=820087 QEP70_RS20280 WP_031599463.1 4416353..4418569(+) (comA) [Pseudomonas amygdali pv. aesculi strain 2250]
ATGCGCACAGGGATGATCGCGCTCGCGTCGGGGCTTGTGGCCTTACGCTTTCTGCCTGCTTTGCCGCCGACCTGGCTGTT
GCTGCTGATGCCGATACTGGCGTTGATGCTGCTACCGTTTCGCACCTATCCACTGGCGCTGTTCCTGCTCGGTTTTACCT
GGGCGTGTGTTTCTGCGCAGTGGGCGTTGAATGACCGTCTGGTTCATCGTCTGGACGGTCAGACGCTGTGGCTGCAAGGC
AAGGTAGTCGGTTTGCCCAGTGTCGCCGAGGGCGTGGTGCGTTTCGAACTGGAAGGGGCAAGGTCGCGCAGGGCGATATT
GCCTGCACGCATCCGGGTGGCGTGGTACGGCGGTCCGCAGGTCAACAGCGGTGAGCGCTGGCGCATGGCGGTCAAACTCA
AGCGGCCAGCCGGGCTGGTCAATCCTGATGCTTTTGATTACGAAGCCTGGCTGTTGGCTCAGCGCATCGGTGCCACCGGC
ACGGTGGTGGATGGTCAACTGCTGACACCTGCGCGCGGGGCGTGGCGCGACCTTATTCGTCAGCGCTTGCTGGCGGTCGA
TGCGCAGGGCAGAGAGGGCGGCTTGGCGGCGCTGGTGCTGGGCGATGGCTCCGGGTTGTCGAGCACCGATTGGCAGGTGC
TGCAGGACACCGGCACGGTGCATCTGCTGGTGATTTCCGGCCAGCATATCGGTTTGCTGGCCGGGGTGATTTACGCGCTG
GTCGCAGGCCTTGCACGCTGGGGGCTGTGGCCACGGGCTTTGCCCTGGCTGCCTTGGGCCTGTGCGCTGGCGTTCATCGC
TGCGCTGGGTTACGGGCTGCTGGCCGGTTTCGAGGTGCCGGTACGGCGTGCCTGTGTGATGGTCGCGATGGTGCTGCTGT
GGCGTTTGCGCTTTCGGCACCTGGGCGTTGTCTGGCCGTTGTTACTGTCATTCAACGCGGTGCTGATTTTCGAGCCGCTG
GTGACACTGCAGCCGGGGTTCTGGCTGTCTTTCGCGGCGGTTGGCATTCTGGTGCTGATTTTCAGTGGACGTCTCGGTGC
CTGGAATTGGTGGCAAAGCTGGACGCGTGCGCAGTGGCTGATCGCAATTGGCTTGCTGCCTATTCTGCTGGCGCTGAACC
TGCCGATCAGCCTCAGCGGGCCGTTTGCCAATTTGCTGGCTGTGCCCTGGGTCAGCTTGATCGTCCTGCCGCCTGCGTTG
CTTGGAACACTGCTGCTGCCGGTCTCTGGTGTGGGCGAAGGCTTGCTCTGGCTGGCCGGAGGGGCGCTGGAGTGGCTGTT
TATGTTTCTCGGCTGGGTTGCCGCAGTCTTGCCCGCCTGGTTGCCCAGTGCAATGCCAGGCTGGGCGTGGCTGCTTAGTC
TGCTCGGGGCCTTCATTTTATTACTGCCCAAAGGCGTGCCGATGCGTCTGCTGGGTTGGCCATTGCTGTTGCTGTGCGTT
TTTCCACCGCTCGACTCAGTGCCTGCAGGCCAGGTAGAAGTATTGCAGCTGGACGTGGGGCAGGGCCTGGCCATTCTCCT
GCGCACTCGAAATCACACGCTCTTGTACGACGCAGGTCCACGTTTCGGCGAGTTCGATATCGGTCAGCGTGTGGTCGTGC
CCGCCATGCGCAAGGCCGGTGTGCGTCATCTGGACCTGATGTTGATCAGTCACGCCGACTCCGATCATGCCGGGGGCGCA
GCGGCGGTTCATCAGGCATTTCCGGTTGACCGTGTACTGGGCGGTGAGCTGGCCAGGCTCGCGCCGCAACTCGACGCGCG
GTTGTGTGAGAGCGATGCGCGCTGGGAGTGGGATGGGGTTATGTTCTCCACCTGGCGCTGGGAGCAGGGCCCTGATGGCA
ATCCGGCATCCTGCATGTTGAGTGTCGACGCAGGGGGCGAGCGTCTGCTGCTGACGGGTGATATAGACGTCGACGCGGAA
CGTGCGGCAATCGCCAGCGGTTTCGACCTTCGTGCCCATTGGTTGCAGTCCCCGCATCACGGCAGCCGGACCTCGTCATC
CAAAGTATTTCTTCAGGCCGTCGGAGCGGTTGGCGTGCTGATCTCCCGTGGCCGTAACAACGCCTTCGGCCACCCGCATC
CGTTGGTCATGGCGCGTTATCGATGGCTGGGTATGGCCAGTTATGACAGCGCTGAGCTGGGCGCTGTCAGTCTGCGGCTG
GGGACGTTCGGCACGCCGCAGGCAGAGCGCGCGCAACGGCGTTTCTGGCGTGACTGA

Domains

Predicted by InterproScan.

Domain of unknown function (DUF4131) (DUF4131)

(21-166)

Metallo-beta-lactamase superfamily (Lactamase_B)

(498-667)

Competence protein (Competence)

(198-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comA Pseudomonas stutzeri DSM 10701

62.151

97.019

0.603
  comA Ralstonia pseudosolanacearum GMI1000

32.787

100

0.379