Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   QAY78_RS02305 Genome accession   NZ_CP123128
Coordinates   428120..428617 (+) Length   165 a.a.
NCBI ID   WP_001796472.1    Uniprot ID   A0A5F0HJ55
Organism   Staphylococcus aureus strain GE-MSSA31     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 423120..433617
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QAY78_RS02265 (QAY78_02260) - 423164..424150 (+) 987 WP_000161307.1 ROK family glucokinase -
  QAY78_RS02270 (QAY78_02265) - 424150..424479 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  QAY78_RS02275 (QAY78_02270) - 424476..425099 (+) 624 WP_001223009.1 MBL fold metallo-hydrolase -
  QAY78_RS02280 (QAY78_02275) comGA 425151..426125 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  QAY78_RS02285 (QAY78_02280) comGB 426097..427167 (+) 1071 WP_000776409.1 competence type IV pilus assembly protein ComGB Machinery gene
  QAY78_RS02290 (QAY78_02285) comGC 427181..427492 (+) 312 WP_000472255.1 competence type IV pilus major pilin ComGC Machinery gene
  QAY78_RS02295 (QAY78_02290) comGD 427470..427916 (+) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  QAY78_RS02300 (QAY78_02295) comGE 427903..428202 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  QAY78_RS02305 (QAY78_02300) comGF 428120..428617 (+) 498 WP_001796472.1 competence type IV pilus minor pilin ComGF Machinery gene
  QAY78_RS02310 (QAY78_02305) - 428714..428860 (+) 147 WP_001789879.1 hypothetical protein -
  QAY78_RS02315 (QAY78_02310) - 428850..429374 (+) 525 WP_001015120.1 shikimate kinase -
  QAY78_RS02320 (QAY78_02315) gcvT 429533..430624 (+) 1092 WP_000093344.1 glycine cleavage system aminomethyltransferase GcvT -
  QAY78_RS02325 (QAY78_02320) gcvPA 430644..431990 (+) 1347 WP_000019698.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  QAY78_RS02330 (QAY78_02325) gcvPB 431983..433455 (+) 1473 WP_000202176.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19169.89 Da        Isoelectric Point: 10.1358

>NTDB_id=819189 QAY78_RS02305 WP_001796472.1 428120..428617(+) (comGF) [Staphylococcus aureus strain GE-MSSA31]
MILSKVTNKYVPFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDKNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=819189 QAY78_RS02305 WP_001796472.1 428120..428617(+) (comGF) [Staphylococcus aureus strain GE-MSSA31]
ATGATATTAAGCAAAGTGACCAACAAATATGTGCCATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAAAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACAATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(68-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5F0HJ55

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.182

100

0.982
  comGF Staphylococcus aureus MW2

95.758

100

0.958