Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrA   Type   Machinery gene
Locus tag   QAY61_RS00605 Genome accession   NZ_CP123115
Coordinates   145596..146945 (-) Length   449 a.a.
NCBI ID   WP_000815638.1    Uniprot ID   Q53IL8
Organism   Staphylococcus aureus strain IT-MRSA43     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 137181..153796 145596..146945 within 0


Gene organization within MGE regions


Location: 137181..153796
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QAY61_RS00565 (QAY61_00565) mecA 137181..139190 (-) 2010 WP_001801873.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  QAY61_RS00570 (QAY61_00570) - 139287..140273 (+) 987 WP_001549960.1 beta-lactam sensor/signal transducer MecR1 -
  QAY61_RS00575 (QAY61_00575) - 140263..140505 (+) 243 Protein_112 type I restriction enzyme endonuclease domain-containing protein -
  QAY61_RS00580 (QAY61_00580) - 140496..142106 (-) 1611 Protein_113 IS1182 family transposase -
  QAY61_RS00585 (QAY61_00585) - 142155..142661 (-) 507 WP_001092188.1 DUF1643 domain-containing protein -
  QAY61_RS00590 (QAY61_00590) - 142676..142987 (-) 312 WP_000700851.1 DUF960 family protein -
  QAY61_RS00595 (QAY61_00595) - 143074..143424 (-) 351 WP_000849672.1 SAUGI family uracil-DNA glycosylase inhibitor -
  QAY61_RS00600 (QAY61_00600) ccrB 143946..145574 (-) 1629 WP_001186608.1 cassette chromosome recombinase CcrB Machinery gene
  QAY61_RS00605 (QAY61_00605) ccrA 145596..146945 (-) 1350 WP_000815638.1 cassette chromosome recombinase CcrA Machinery gene
  QAY61_RS00610 (QAY61_00610) cch1 147179..148972 (-) 1794 WP_000195099.1 cassette chromosome replicative helicase -
  QAY61_RS00615 (QAY61_00615) ssb 148972..149268 (-) 297 WP_000221966.1 cassette chromosome ssDNA-binding protein -
  QAY61_RS00620 (QAY61_00620) - 149440..151023 (+) 1584 WP_000271876.1 hypothetical protein -
  QAY61_RS00625 (QAY61_00625) - 151095..152027 (+) 933 WP_000364405.1 hypothetical protein -
  QAY61_RS00630 (QAY61_00630) - 152453..152830 (+) 378 WP_000687965.1 hypothetical protein -
  QAY61_RS00635 (QAY61_00635) - 153299..153796 (+) 498 WP_000051479.1 hypothetical protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 52636.23 Da        Isoelectric Point: 9.9639

>NTDB_id=818967 QAY61_RS00605 WP_000815638.1 145596..146945(-) (ccrA) [Staphylococcus aureus strain IT-MRSA43]
MKQVIGYLRQSTMKQQSLAAQKQAIEAIAEKHHIQHINFYSDKQSGRKDNRSGYRQITQLIQQGQCDILCCYRLNRLHRN
LKNALKLIKLCQTYHVHILSVHDGYFDMDQAFDRLKLNIFISLAELESDNIGEQVRNGLQEKAKQGRLITTHAPFGYDYH
HGTFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYCGRVNNQYGQFDNMFPSIVSTSI
YEQAQRLRSQKQTKQTSSDNQLKQKIKCPCCNATLTNMTIRKKNHTLRYYVCPKNMNASRFVCDFKGINAQTLEDKVLEV
CRDFYQNQRIYTKIKSAIDKRIKRQRNIEKHHTLTQEQLIEKLAQGIIDAETFREQTQSLRQQPQRTTSINGHQIQHIIQ
NIIQKRFTLNILYPYIETIHITKDKNLIGIYFKNEPLNIVNQTMQSSIA

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=818967 QAY61_RS00605 WP_000815638.1 145596..146945(-) (ccrA) [Staphylococcus aureus strain IT-MRSA43]
ATGAAACAAGTCATAGGCTATTTACGTCAAAGTACGATGAAACAACAATCTCTTGCAGCACAGAAACAAGCTATCGAAGC
AATAGCCGAAAAACATCATATTCAACATATCAACTTTTATAGCGACAAACAATCAGGTCGCAAAGATAATCGTAGTGGGT
ATCGACAAATAACACAATTAATTCAACAAGGACAGTGTGACATATTATGCTGTTATCGTCTTAATAGGTTGCATCGTAAT
CTGAAAAATGCATTAAAACTCATCAAATTATGTCAAACGTACCATGTTCATATCTTAAGCGTACATGATGGTTACTTTGA
TATGGATCAAGCTTTCGACCGACTCAAGCTTAATATCTTCATCAGTTTAGCCGAACTTGAATCGGATAACATTGGAGAAC
AAGTCAGAAATGGGCTTCAAGAAAAAGCAAAGCAAGGTCGATTGATTACAACACATGCACCTTTTGGTTACGATTATCAC
CACGGAACATTCATCATCAATCAAAATGAGTCACCAACGGTAAAGGCTGTATTCAATTATTACATTAAAGGCCATGGTTA
TAAGAAAATTGCACAGTTATTAGAAGAAGATAACACGTATATCAATCGACAACCCTATCAAGTTCGTAACATTATCATCA
ATCCTAATTATTGTGGTCGTGTCAACAATCAATATGGCCAATTCGACAATATGTTTCCTTCTATTGTTTCCACAAGTATA
TATGAGCAAGCGCAGAGACTTCGATCGCAAAAACAAACCAAACAGACATCTTCGGATAATCAACTCAAACAAAAAATCAA
ATGCCCATGTTGTAATGCAACACTTACAAATATGACCATTAGAAAAAAGAATCATACATTACGTTACTACGTCTGTCCTA
AAAACATGAATGCTTCACGCTTTGTCTGTGATTTTAAAGGCATCAATGCACAAACACTTGAAGATAAAGTATTAGAAGTG
TGCCGAGACTTTTATCAAAATCAACGCATCTACACAAAAATTAAAAGTGCGATTGACAAACGCATCAAAAGACAAAGAAA
CATAGAAAAACATCACACATTGACTCAAGAACAACTGATAGAAAAGTTGGCACAAGGCATCATTGATGCAGAAACGTTCA
GAGAGCAAACACAATCATTACGTCAACAACCGCAACGCACTACATCTATCAATGGGCATCAAATACAACACATCATTCAA
AATATTATTCAAAAACGTTTCACGTTAAACATATTGTACCCCTATATTGAAACCATTCACATTACGAAAGATAAAAATCT
TATAGGAATCTATTTCAAAAATGAACCACTCAATATTGTCAATCAAACCATGCAATCATCGATTGCATAA

Domains

Predicted by InterproScan.

Resolvase, N terminal domain (Resolvase)

(4-147)

Recombinase (Recombinase)

(169-250)

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(262-321)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q53IL8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrA Staphylococcus aureus N315

97.55

100

0.976
  ccrA Staphylococcus aureus COL

75.501

100

0.755