Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   QAO32_RS02370 Genome accession   NZ_CP123070
Coordinates   416526..417212 (+) Length   228 a.a.
NCBI ID   WP_072468915.1    Uniprot ID   A0A5F0HFN7
Organism   Staphylococcus aureus strain SW-MSSA64     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 411526..422212
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QAO32_RS02335 (QAO32_02330) yqeH 412053..413153 (+) 1101 WP_001280146.1 ribosome biogenesis GTPase YqeH -
  QAO32_RS02340 (QAO32_02335) aroE 413167..413973 (+) 807 WP_000666750.1 shikimate dehydrogenase -
  QAO32_RS02345 (QAO32_02340) yhbY 413977..414267 (+) 291 WP_000955234.1 ribosome assembly RNA-binding protein YhbY -
  QAO32_RS02350 (QAO32_02345) nadD 414270..414839 (+) 570 WP_000725165.1 nicotinate (nicotinamide) nucleotide adenylyltransferase -
  QAO32_RS02355 (QAO32_02350) yqeK 414829..415413 (+) 585 WP_001017837.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  QAO32_RS02360 (QAO32_02355) rsfS 415414..415767 (+) 354 WP_001088020.1 ribosome silencing factor -
  QAO32_RS02365 (QAO32_02360) - 415770..416486 (+) 717 WP_000084840.1 class I SAM-dependent DNA methyltransferase -
  QAO32_RS02370 (QAO32_02365) comEA 416526..417212 (+) 687 WP_072468915.1 ComEA family DNA-binding protein Machinery gene
  QAO32_RS02375 (QAO32_02370) - 417304..417765 (+) 462 WP_000439693.1 ComE operon protein 2 -
  QAO32_RS02380 (QAO32_02375) comEC 417770..419971 (+) 2202 WP_001622275.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  QAO32_RS02385 (QAO32_02380) holA 420028..421002 (+) 975 WP_001282573.1 DNA polymerase III subunit delta -
  QAO32_RS02390 (QAO32_02385) rpsT 421047..421298 (-) 252 WP_001274017.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 228 a.a.        Molecular weight: 25812.41 Da        Isoelectric Point: 9.3305

>NTDB_id=818120 QAO32_RS02370 WP_072468915.1 416526..417212(+) (comEA) [Staphylococcus aureus strain SW-MSSA64]
MVLLYQFLLRYKDFLTQWKLYIISAVVLIMVLIGFIFWRQDDYTSRDFENKDTALKQSTSENSSLSKLEDVQAKDGDNSK
NKGPVYVDIKGAVKHPNVYKMTSKDRVVDLLDKAQLLDDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQFGENSVHVKN
GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLKSYFTT

Nucleotide


Download         Length: 687 bp        

>NTDB_id=818120 QAO32_RS02370 WP_072468915.1 416526..417212(+) (comEA) [Staphylococcus aureus strain SW-MSSA64]
GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAACTCAATGGAAGTTATATATTATAAGTGCTGTTGT
TTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAAGATGATTATACTTCAAGAGATTTTGAAAATAAAGATACTG
CTCTGAAACAAAGCACTAGTGAAAATAGTAGTTTGTCCAAATTAGAAGATGTCCAGGCCAAAGATGGAGATAATTCCAAA
AATAAAGGTCCTGTATATGTCGATATAAAAGGTGCTGTTAAACACCCTAATGTTTATAAAATGACATCTAAGGATAGAGT
AGTTGATTTACTTGATAAAGCACAATTATTGGATGATGCAGATGTAAGTCAAATTAATTTGTCTGAAAAATTAACAGATC
AAAAAATGATTTTCATTCCTCATAAAGGACAAAAGAATGTTGAACCACAATTTGGAGAAAACAGTGTGCACGTAAAAAAT
GGGAACACAAATAATACTAAAGTAAATTTAAATACGGCATCTGTATCAGAATTGATGTCTGTTCCTGGAGTAGGGCAAGC
TAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGTGCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTT
TTGGAAGTAAAACTTTTGATAAACTGAAATCTTATTTCACGACATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(164-227)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5F0HFN7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Staphylococcus aureus MW2

96.476

99.561

0.961
  comEA Staphylococcus aureus N315

96.476

99.561

0.961