Detailed information NTG0818055

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: QEP67_RS22150
• Genome accession: NZ_CP123058
• Coordinates: 4268103..4271828 (-)
• Length: 1241 a.a.
• NCBI ID: WP_064459983.1
• Uniprot ID: A0A1G6Y2G5
• Organism: Bacillus cereus group sp. MS39
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 4263103..4276828

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QEP67_RS22100 (QEP67_22025)	-	4263996..4264280 (+)	285	WP_000926860.1	hypothetical protein	-
QEP67_RS22105 (QEP67_22030)	-	4264315..4265214 (-)	900	WP_001241719.1	fumarylacetoacetate hydrolase family protein	-
QEP67_RS22110 (QEP67_22035)	-	4265461..4265640 (+)	180	WP_000462847.1	aspartyl-phosphate phosphatase Spo0E family protein	-
QEP67_RS22115 (QEP67_22040)	gerPA	4265735..4265956 (+)	222	WP_001111187.1	spore germination protein GerPA	-
QEP67_RS22120 (QEP67_22045)	gerPB	4265971..4266177 (+)	207	WP_001012506.1	spore germination protein GerPB	-
QEP67_RS22125 (QEP67_22050)	gerPC	4266246..4266860 (+)	615	WP_001070746.1	spore germination protein GerPC	-
QEP67_RS22130 (QEP67_22055)	gerPD	4266867..4267061 (+)	195	WP_001052804.1	spore germination protein GerPD	-
QEP67_RS22135 (QEP67_22060)	-	4267077..4267463 (+)	387	WP_000902337.1	spore germination protein GerPE	-
QEP67_RS22140 (QEP67_22065)	gerPF	4267506..4267721 (+)	216	WP_001141570.1	spore germination protein GerPF	-
QEP67_RS22145 (QEP67_22070)	-	4267803..4268090 (-)	288	WP_064459982.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
QEP67_RS22150 (QEP67_22075)	addA	4268103..4271828 (-)	3726	WP_064459983.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
QEP67_RS22155 (QEP67_22080)	addB	4271825..4275340 (-)	3516	WP_000058580.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
QEP67_RS22160 (QEP67_22085)	lepB	4275457..4276020 (-)	564	WP_000751908.1	signal peptidase I	-
QEP67_RS22165 (QEP67_22090)	-	4276217..4276810 (-)	594	WP_000347517.1	TVP38/TMEM64 family protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142848.07 Da
• Isoelectric Point: 4.8199

>NTDB_id=818055 QEP67_RS22150 WP_064459983.1 4268103..4271828(-) (addA) [Bacillus cereus group sp. MS39]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHVRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNSIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEAHI
RKATELAMLPDGPAPRVETLQADAVLLGTLSSAARESWTSVYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRRPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSESGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLDGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGATYPEGEDVAAELLCIQQTEEEVIDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYTVYDRKTNEMRQVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSTFLKGAPLEEEQELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGSIPGEIYEYDTNWK
VEVVDGKTLLAPEPVQEEKQELLEALREKKAVPLQSERKEEVYDRLMWKYGYEEATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITIEVLQEQIAGMVNRELLTFEQAEEVAIEKVISFFDS
DLGKRVLAAESVEREVPFTMMLAAEEAYQDWQGKSDETILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFDQAKPIL
EDRYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVVKVEE

Nucleotide

• Length: 3726 bp

>NTDB_id=818055 QEP67_RS22150 WP_064459983.1 4268103..4271828(-) (addA) [Bacillus cereus group sp. MS39]
ATGATTGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAGTGGACGGATGACCAGTGGAAAGCGGTTGTAGCGAACGGACG
TGATATATTAGTCGCAGCAGCAGCTGGATCAGGGAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATAAATG
AAGAAAACCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCGGCGCAAGAGATGAAAAACAGAATT
GGGGAAGCGCTAGAAAAAGTATTAATTGATGAGCCAGGATCGCAGCACGTAAGAAAGCAGCTTAGCTTATTAAATAAAGC
TTCTATTTCGACAATCCATTCCTTTTGTTTACAAGTAATTAGAGGATACTATTACATGCTTGATGTGGATCCTCGTTTCC
GCATTGCGAATCAAACAGAAAACGAATTATTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAGTATGGAATCGAA
GATAATAGTATTTTCTTTGAACTCGTTGATCGTTATACGAGTGACCGTAGTGATGATGACTTACAGCGTATGATTTTAGC
GCTTCATACAGAATCAAGAGCGCATCCAAATCCGGAAAAATGGCTCGATAAATTAGTAGAAGCATACGACGTGGAAGGAA
AGACAATTGAAGATTTAGTGTATGCTTCTTACTTATTAGAAGATGTGAAATTCCAGCTTGAAACAGCAGAGGCACATATT
CGTAAAGCGACTGAACTCGCAATGCTTCCTGACGGTCCGGCACCCCGCGTTGAAACACTGCAAGCTGATGCAGTTTTACT
TGGAACGTTGTCATCAGCTGCTCGTGAGTCATGGACAAGTGTGTATGAAGCGATGCAAAATGTATCGTGGCAAACGTTAA
AGCGCATTAAGAAAAGTGATTACAACGAGGATGTTGTAAAACAAGTGGACTCTCTTCGTAATAAAGCGAAAGATGAAGTG
AAGAAATTACAAGAAGAGTTATTTAGCCGCAGACCTGAAAGTTTCTTACGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAACTCGTACAGCTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAGCGGGATAAAGGAATGGTCGATTTCACAG
ATTTAGAGCATTTCTGCTTACAAATTTTAAGTGAACAAAGTGAAAGTGGTGAAATGAAGCCATCAGCAGTAGCACTTCAA
TATCGTAATAAATTTGCTGAAGTACTAGTTGATGAATATCAAGATACGAACTTTGTGCAGGAATCCATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGGAACTTGTTCATGGTAGGTGACGTGAAGCAGTCGATCTATCGTTTCCGACTAGCAG
AACCAGGCCTGTTCCTAGGAAAGTATAAACGCTTTACGCAAGAAGGATTAGACGGCGGAATGAAAATCGATTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTGTTAGCAGGTACGAACTTTATTTTCAAACAAATTATGGGTGAAGAAGTCGGAGAAAT
TGATTACGATGCTGACGCTGAATTAAAGTTAGGTGCTACCTATCCAGAAGGTGAAGATGTAGCGGCAGAACTACTATGCA
TTCAGCAAACGGAAGAAGAGGTAATAGACGGAGAAGAAGGTGCAGAAGTCGAAAAAGCACAGCTTGAAGCTCGCCTTATG
GCGCAGCGAATAAAGGCTATGGTTGATTCAGGATATACCGTCTATGATCGCAAAACGAATGAAATGCGACAAGTACAATA
CCGCGACTTCGTTATTTTACTTCGCTCCATGCCATGGGCGCCGCAAATTATGGAAGAGTTAAAGCTGCAAGGAATTCCAG
TATACGCTGATCTTGCGACTGGTTACTTTGAAGCGACAGAAGTAAATATTATGATGAACGTCTTCCGCGTGATCGATAAT
CCGATGCAAGATATTCCGCTTGCCGCCGTACTTCGTTCACCAATCGTTGGGTTAAATGATGAGGAACTTGCGACGCTTCG
TGCTCACGGGAAGAAAGGTTCGTTTTATGAAGTAATGAGCACATTCTTAAAAGGGGCACCACTTGAAGAAGAGCAAGAAC
TACATGATAAATTAGAGTGGTTCTATAACTTACTGCAAGGATGGCGTGAATTCGCGCGTCAACAGTCACTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGTTATTACGACTTTGTTGGCGGTTTACCAGCCGGAAAGCAAAGGCAAGCAAACCT
GCGTGTACTTTATGACCGTGCAAGACAATATGAAGCAACATCGTTTAGAGGATTATTCCGCTTCTTACGCTTTATTGAGC
GTATTTTAGAACGCGGTGATGATATGGGAACTGCGAGAGCTTTAGGTGAACAAGAAGATGTCGTTCGTATTATGACGATT
CATAAAAGTAAAGGACTTGAGTTCCCAGTCGTATTTGTAGCGGGACTAGGTCGTCGTTTTAATACACAAGATTTAATGAA
ACGTTTCTTACTGCATAAAGACTTCGGTTTCGGTTCACAATTTATCGATCCTCGTAAACGAATTAAATATACGACATTAT
CGCAACTAGCAATTAAGCGTAAAATGAAAATGGAATTAATCGCGGAAGAAATGCGCGTGTTATACGTAGCGTTAACGCGT
GCGAAAGAGAAGTTAATTTTAATTGGAACGGTTAAAGATGCCAATAAAGAAATGGAAAAATGGCTCGATGCAAGGGAGCA
TAGTGAATGGTTATTACCAGACCATATACGTGCCGGAGCTTCTTGTTATTTAGACTGGATTGCACCTTCATTATATAGAC
ATCGTGACAGTGAAATGCTTCTTGAATTAGGACAAGGAAGCATTCCAGGTGAAATTTATGAATATGACACGAACTGGAAA
GTGGAAGTTGTTGACGGCAAAACGTTACTTGCACCGGAACCGGTTCAAGAAGAGAAACAAGAATTGTTAGAAGCACTTCG
TGAGAAAAAAGCTGTTCCGCTACAAAGTGAACGGAAAGAAGAAGTGTACGACAGATTAATGTGGAAGTACGGATATGAGG
AAGCAACATCTCACCGTGCGAAACAGTCTGTTACAGAAATAAAGAGAAACTACCAATCAGAAGAAGGTAGCGATAATGCC
TTTATTAAAAAACTGCGTGCACCAATTAAAACGCGTCCTCGTTTTATGGAGAAAAAAGGATTGACGTACGCAGAGCGTGG
GACAGCAGTCCATGCTGTTATGCAACATGTAGATTTGAAGAAACCAATTACGATTGAAGTTCTTCAAGAGCAAATTGCCG
GAATGGTAAATAGGGAATTATTAACATTCGAACAAGCTGAAGAAGTAGCAATTGAAAAAGTGATTTCATTCTTTGACAGT
GACCTAGGTAAGAGGGTATTAGCGGCGGAAAGTGTTGAGCGTGAAGTACCATTTACGATGATGCTTGCAGCAGAAGAGGC
GTATCAAGATTGGCAAGGTAAGAGTGACGAAACGATACTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAGGAAGATG
GTATCACTTTAATCGACTTTAAAACGGATACGATTGAAGGGAAGTTCCCAGGCGGATTCGATCAAGCGAAACCGATTTTA
GAAGATCGATATAAAGTACAGCTTTCGTTATATGCAAAGGCGCTTGAAAAAAGCTTGCAACATCCTGTGAAAGAGAAATG
CTTATACTTCTTTGATGGCAATCATGTTGTCAAAGTTGAGGAATAG

Domains

Predicted by InterproScan.

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1060-1232)

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

3D structure

• AlphaFold DB: A0A1G6Y2G5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	52.927	100	0.532