Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   QEP67_RS22155 Genome accession   NZ_CP123058
Coordinates   4271825..4275340 (-) Length   1171 a.a.
NCBI ID   WP_000058580.1    Uniprot ID   A0A4R4BET5
Organism   Bacillus cereus group sp. MS39     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4266825..4280340
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QEP67_RS22130 (QEP67_22055) gerPD 4266867..4267061 (+) 195 WP_001052804.1 spore germination protein GerPD -
  QEP67_RS22135 (QEP67_22060) - 4267077..4267463 (+) 387 WP_000902337.1 spore germination protein GerPE -
  QEP67_RS22140 (QEP67_22065) gerPF 4267506..4267721 (+) 216 WP_001141570.1 spore germination protein GerPF -
  QEP67_RS22145 (QEP67_22070) - 4267803..4268090 (-) 288 WP_064459982.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  QEP67_RS22150 (QEP67_22075) addA 4268103..4271828 (-) 3726 WP_064459983.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  QEP67_RS22155 (QEP67_22080) addB 4271825..4275340 (-) 3516 WP_000058580.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  QEP67_RS22160 (QEP67_22085) lepB 4275457..4276020 (-) 564 WP_000751908.1 signal peptidase I -
  QEP67_RS22165 (QEP67_22090) - 4276217..4276810 (-) 594 WP_000347517.1 TVP38/TMEM64 family protein -
  QEP67_RS22170 - 4277161..4277397 (-) 237 WP_000152648.1 hypothetical protein -
  QEP67_RS22175 (QEP67_22095) - 4277641..4278222 (-) 582 WP_000444426.1 competence protein ComK -
  QEP67_RS22180 (QEP67_22100) - 4278506..4278691 (+) 186 WP_000391438.1 hypothetical protein -
  QEP67_RS22185 (QEP67_22105) cspA 4278944..4279147 (-) 204 WP_000301521.1 RNA chaperone/antiterminator CspA -
  QEP67_RS22190 (QEP67_22110) - 4279521..4280312 (+) 792 WP_000074028.1 phosphotransferase -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134367.46 Da        Isoelectric Point: 5.1777

>NTDB_id=818056 QEP67_RS22155 WP_000058580.1 4271825..4275340(-) (addB) [Bacillus cereus group sp. MS39]
MSLRFVIGRAGSGKSTLCLHEVQEELKQRPRGKTILYLVPEQMTFQTQQALIGSEDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKDGLSVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDAHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLVEKLPQSEYVNGAEIYIDGFHSFSPQELEIVRQLMICGARVTITLTIDE
KTLAQPVNELDLFYETTLTYEKIKQVAREEKIEIEKTIPLMEQPRFHSQALAHLETHYEARPNEKFHGEASVTISTAANL
RAEVEGVAREIRRLVADEDYRYRDIAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKETMREEMDEFENYCLAYGVQGKRWTSEDPWMYRRYRSLDDTDEMITDSEREMEERINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEALRIRAEESGDFLFATDHEQVWEEVMSLLDTFVEMLGEEK
MSLSMFTDVMSTGLEALQFANIPPSLDQVLIANIDRSRLSNVKATFVIGVNEGVIPAAPMDEGMLSDEERDVLSVAGIEL
APTTRQTLLEEQFVMYQMVTRATEKLYISCPLADEEGKTLLASSFIKKIKRMFPDVKDTFITNDVNDLSRSEQISYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAQKLSTVVSRDLYGDKIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKRIADRLLRENRTWADLSIKECEHLSAVVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSIILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFSLPNGVKMEVVGRIDRVDKAEDENG
TFLRIIDYKSSSKALDLTEVYYGLALQMLTYLDVVTSNAHTWMKKGGTASPAGVLYFHIHNPIVEMKGDASEAEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNVLQKYVHHTFENIGKDITEGVIDIAP
YKKGNKAACTFCNFKSVCQFDESLEDNQFRTLKDMKDSEAMEKIREEVGGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=818056 QEP67_RS22155 WP_000058580.1 4271825..4275340(-) (addB) [Bacillus cereus group sp. MS39]
ATGTCACTTCGATTTGTAATTGGTAGAGCGGGAAGTGGAAAAAGTACACTTTGTTTACACGAAGTGCAGGAAGAGTTAAA
GCAACGCCCAAGAGGGAAAACAATATTATATCTTGTGCCAGAACAGATGACATTCCAAACGCAGCAGGCGTTAATTGGTA
GTGAGGATGTAAGAGGTTCTATTCGGGCACAAGTTTTTAGTTTTTCACGATTAGCGTGGAAGGTACTGCAAGAAGTTGGT
GGAGCGAGTCGTCTTCACATTGATGAAGCGGGCGTACATATGTTACTTCGTAAAATTGTAGAGTCTCGTAAAGATGGATT
ATCGGTGTTCCAAAAAGCAGCGGAGCAAAACGGTTTCTTTGAACATCTTGGCAGTATGATTGCGGAGTTTAAACGTTACA
ACGTGACGCCATCTAACGTATATGAAATGTGGCAACAATTAGATGCGCATAGTAGCAGTGCAGAACAAAAGCTATTAGCG
AATAAAGTGTATGATTTACAACTATTATATGATGATTTTGAACGTGCTTTAATCGGAAAATATTTGGATTCAGAAGACTA
CCTACAATTGCTAGTCGAAAAGCTTCCACAGTCTGAATATGTAAATGGGGCTGAAATTTATATAGATGGATTTCATTCAT
TTTCACCGCAAGAACTAGAAATTGTAAGACAGCTTATGATTTGCGGTGCGCGAGTTACGATCACGTTAACGATAGATGAA
AAAACGTTAGCACAGCCAGTAAATGAACTAGATTTATTTTATGAAACGACGTTAACGTATGAAAAAATAAAGCAAGTGGC
GCGTGAAGAGAAGATAGAAATTGAAAAAACAATTCCACTTATGGAACAACCGCGTTTTCATTCGCAAGCATTAGCGCATT
TAGAAACGCATTACGAAGCACGTCCAAATGAAAAGTTTCACGGAGAAGCAAGTGTAACGATTAGTACAGCAGCGAATTTA
CGAGCTGAAGTAGAAGGTGTTGCCCGTGAAATTCGTAGACTTGTGGCAGATGAAGACTATCGTTACCGAGATATTGCAGT
CCTTCTTCGTAACGGAGAAAGTTATTACGATGTGATGCGAACGCTATTTACAGATTATAATATCCCGCATTTCATCGATG
AAAAACGCCCGATGTCACATCATCCGCTAGTAGAATGCATTCGTTCTGCACTCGAGATTATTAGCGGAAATTGGCGTTAT
GATGCAGTGTTCCGCTGCGTGAAAACAGAGCTTTTATATCCATTAGACGTAAGAAAAGAAACGATGCGCGAAGAGATGGA
TGAGTTTGAAAACTACTGTTTAGCGTACGGTGTACAAGGGAAGAGATGGACTTCTGAGGATCCGTGGATGTATCGCCGCT
ATCGTTCTCTTGACGATACAGACGAGATGATTACAGACAGTGAACGTGAAATGGAAGAGAGAATAAATCGACTGCGCGAC
GTTGTAAGAACGCCTGTTATTCGTATGCAAAAAAGGCTGAAGCGAGCGGGAACAGTTATGCAAATGTGCGAAGCTGTTTA
TTTATTTTTAGAAGAGCTAGACGTTCCGAAAAAACTAGAAGCATTACGTATTCGTGCAGAAGAGAGCGGAGATTTCTTAT
TTGCGACAGATCATGAGCAAGTATGGGAAGAAGTAATGAGTCTTCTTGATACGTTCGTTGAGATGCTTGGCGAAGAGAAA
ATGTCACTTTCTATGTTCACAGACGTTATGTCGACAGGTCTTGAGGCGCTTCAATTTGCAAACATTCCGCCGTCATTAGA
CCAAGTGTTAATTGCTAATATTGATCGTTCTAGATTGTCAAATGTGAAAGCAACATTTGTCATTGGCGTGAATGAAGGAG
TTATTCCGGCAGCACCGATGGATGAAGGGATGCTTTCTGATGAGGAAAGAGATGTTCTTAGTGTTGCAGGTATCGAATTA
GCACCAACGACGAGACAAACCTTATTAGAAGAACAGTTCGTTATGTACCAAATGGTAACGAGAGCAACTGAGAAATTATA
TATTTCATGCCCGCTTGCAGATGAGGAAGGGAAGACGTTACTTGCGTCTAGCTTTATTAAGAAAATAAAAAGAATGTTCC
CTGATGTGAAAGATACATTTATTACAAATGACGTAAATGATTTATCACGTTCGGAACAAATTTCATACGTAGCAACGCCA
GAAGTAACGTTGTCATATGTTATGCAGCAACTTCAAACGTGGAAGCGATATGGATTTGAAGGGAATTTAGATTTTTGGTG
GGATGTATATAACTTCTACGTAACTTCAGATGAATGGAAACAAAAAAGTAGCCGCGTATTATCAAGTTTATTCTACCGAA
ATCGTGCGCAAAAGCTAAGTACAGTTGTAAGTAGAGATTTATATGGAGATAAAATAAAAGGAAGCGTATCTCGTATGGAA
CTATTTAACCGTTGCGCATATGCTCATTTCGCGCAGCACGGTTTATCGTTAAGAGAGCGTGACATTTTCAAATTGGATGC
GCCAGATATCGGGGAACTATTCCATGCAGCGCTGAAGAGAATTGCAGACAGGCTATTACGTGAAAACCGTACTTGGGCGG
ATTTATCAATAAAAGAGTGTGAGCATCTTTCGGCTGTAGTAATAGAAGAGATTGCACCGTTATTACAAAGACAAATTTTA
TTAAGTTCAAATCGTCATTTCTATTTAAAACAAAAACTACAACAAATCATTTTCCGTACATCAATCATTCTTCGTGAACA
TGCGAAGTCAAGTGGTTTCGTACCAGTTGATTTAGAAGTGCCATTCGGTATGGGCGGTACAGGATCGCTTCCGCCGATGG
AATTCTCATTACCAAATGGCGTGAAGATGGAAGTAGTTGGCCGTATTGACCGTGTTGATAAGGCAGAAGATGAAAACGGG
ACGTTCCTACGTATTATTGACTATAAATCAAGCTCAAAAGCGTTAGACTTAACAGAAGTGTATTACGGATTAGCACTTCA
AATGTTAACGTATTTAGATGTTGTTACTTCAAATGCACATACGTGGATGAAAAAGGGAGGTACTGCATCACCGGCTGGTG
TACTGTACTTCCATATTCATAACCCGATCGTTGAGATGAAGGGTGACGCATCTGAAGCAGAAATTGAGAAAGAGATTTTA
AAGAAATTTAAAATGAAAGGACTCGTACTAGGAGATGCTGATGTTGTTCGTTTAATGGATAACAAACTTTCAACAGGAAG
CTCTGATATTATTTCTGCTGGTTTGAAAAAAGACGGTAGCTTTAGTGCGCGTTCAAGCATTGCAAGTGAACAAGAGTTTA
ACGTACTGCAAAAATACGTACACCATACGTTTGAAAATATCGGAAAAGACATTACAGAAGGCGTTATTGATATTGCTCCT
TATAAGAAGGGGAATAAAGCGGCGTGTACGTTCTGTAACTTCAAATCTGTTTGTCAGTTCGATGAATCACTTGAAGATAA
CCAGTTCCGTACGCTAAAAGATATGAAAGATAGCGAAGCGATGGAGAAAATTAGAGAGGAGGTTGGCGGAGAATGA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(303-669)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4R4BET5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.233

100

0.494