Detailed information NTG0817676

Bioinformatically predicted

Overview

• Name: dprA
• Type: Machinery gene
• Locus tag: LLUL033_RS06400
• Genome accession: NZ_CP122980
• Coordinates: 1248226..1249074 (-)
• Length: 282 a.a.
• NCBI ID: WP_011835152.1
• Uniprot ID: Q25AZ2
• Organism: Lactococcus cremoris strain UL033
• Function: ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 1243226..1254074

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLUL033_RS06385 (LLUL033_06370)	xerS	1243296..1244366 (+)	1071	WP_011835149.1	tyrosine recombinase XerS	-
LLUL033_RS06390 (LLUL033_06375)	trmFO	1244416..1245762 (-)	1347	WP_011835150.1	methylenetetrahydrofolate--tRNA-(uracil(54)- C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO	-
LLUL033_RS06395 (LLUL033_06380)	topA	1245834..1247960 (-)	2127	WP_259691981.1	type I DNA topoisomerase	-
LLUL033_RS06400 (LLUL033_06385)	dprA	1248226..1249074 (-)	849	WP_011835152.1	DNA-processing protein DprA	Machinery gene
LLUL033_RS06405 (LLUL033_06390)	-	1249222..1249602 (-)	381	WP_011835153.1	RidA family protein	-
LLUL033_RS06410 (LLUL033_06395)	budA	1249642..1250352 (-)	711	WP_011835154.1	acetolactate decarboxylase	-
LLUL033_RS06415 (LLUL033_06400)	ilvA	1250488..1251738 (-)	1251	WP_011835155.1	threonine ammonia-lyase IlvA	-
LLUL033_RS06420 (LLUL033_06405)	-	1251797..1252357 (-)	561	Protein_1238	ketol-acid reductoisomerase	-
LLUL033_RS06425 (LLUL033_06410)	-	1252377..1252580 (-)	204	Protein_1239	isocitrate/isopropylmalate family dehydrogenase	-

Sequence

Protein

• Length: 282 a.a.
• Molecular weight: 31919.06 Da
• Isoelectric Point: 9.9168

>NTDB_id=817676 LLUL033_RS06400 WP_011835152.1 1248226..1249074(-) (dprA) [Lactococcus cremoris strain UL033]
MITNFDLFRWKKAGMINLGVNKLLKFFHKYDRKISLRQMGQVAQVKSIPNFIEQYKNQDVKKLRIDYKKFSSFSILDDLY
PERLREIYNPPVLIFYQGNIDLLKNPKLAFVGSRLAGQSGIKAVQKIVTELNQSFTIVSGLAKGIDTASHLSAIKTKTPT
IAVIGTGLDIFYPLENRKIQEYLAKNQLVLSEYSLGEKPLKYHFPERNRIIAGLSRGVVVVEAKLRSGSLITCERALEEG
RDIFAIPGNIADGTSDGCNHLIQQGAKLVYQAQDILEEYLYN

Nucleotide

• Length: 849 bp

>NTDB_id=817676 LLUL033_RS06400 WP_011835152.1 1248226..1249074(-) (dprA) [Lactococcus cremoris strain UL033]
ATGATTACAAATTTTGATTTGTTTCGTTGGAAAAAAGCAGGAATGATCAATCTTGGTGTAAATAAGCTCTTAAAATTTTT
CCACAAATATGATAGAAAAATAAGCTTACGACAAATGGGACAAGTGGCTCAAGTCAAATCAATCCCTAACTTTATCGAAC
AATATAAAAATCAGGACGTAAAGAAACTCAGGATAGACTATAAAAAATTTTCCTCCTTTTCCATTCTTGATGACCTTTAT
CCCGAGCGTCTAAGAGAAATCTATAACCCACCCGTCTTAATTTTTTATCAGGGAAATATTGATTTATTAAAAAACCCTAA
ACTTGCCTTTGTTGGAAGCCGCCTAGCAGGTCAATCAGGGATAAAAGCTGTCCAAAAAATTGTGACAGAATTGAATCAAT
CCTTTACAATAGTAAGCGGACTTGCCAAAGGGATTGACACAGCCAGCCATCTGTCAGCAATTAAAACTAAAACACCAACA
ATTGCTGTTATTGGTACAGGATTGGATATTTTCTATCCACTAGAAAATCGTAAAATACAGGAATATCTAGCAAAAAATCA
ATTAGTTTTAAGTGAATATTCATTAGGCGAAAAACCACTTAAATACCACTTCCCAGAGCGCAATCGAATTATTGCTGGTC
TTTCACGGGGAGTTGTTGTCGTGGAAGCAAAATTAAGAAGTGGAAGTTTAATTACCTGTGAAAGGGCACTGGAAGAAGGA
AGAGATATCTTTGCCATCCCTGGAAATATTGCAGATGGAACATCTGATGGCTGTAATCATCTTATTCAACAAGGAGCAAA
ACTTGTTTATCAAGCTCAAGATATTTTAGAAGAATATTTGTACAATTAA

Domains

Predicted by InterproScan.

• DNA recombination-mediator protein A (DNA_processg_A) (69-270)

• DNA processing protein A sterile alpha motif domain (SAM_DprA) (2-63)

3D structure

• AlphaFold DB: Q25AZ2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
dprA	Lactococcus lactis subsp. cremoris KW2	98.227	100	0.982
dprA	Streptococcus mutans UA159	58.633	98.582	0.578
dprA/cilB/dalA	Streptococcus mitis SK321	55.357	99.291	0.55
dprA/cilB/dalA	Streptococcus pneumoniae R6	55	99.291	0.546
dprA/cilB/dalA	Streptococcus mitis NCTC 12261	55	99.291	0.546
dprA/cilB/dalA	Streptococcus pneumoniae TIGR4	55	99.291	0.546
dprA/cilB/dalA	Streptococcus pneumoniae Rx1	55	99.291	0.546
dprA/cilB/dalA	Streptococcus pneumoniae D39	55	99.291	0.546
dprA	Legionella pneumophila strain ERS1305867	39.13	97.872	0.383
dprA	Haemophilus influenzae Rd KW20	39.925	95.035	0.379