Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   M2042_RS14950 Genome accession   NZ_AP023187
Coordinates   3106456..3107472 (-) Length   338 a.a.
NCBI ID   WP_005381324.1    Uniprot ID   A0AAW9BNQ7
Organism   Vibrio alginolyticus strain HLBS-07     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3101456..3112472
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M2042_RS14925 (HLBS07_28500) aroK 3102329..3102847 (-) 519 WP_005381319.1 shikimate kinase AroK -
  M2042_RS14930 (HLBS07_28510) pilQ 3103035..3104780 (-) 1746 WP_248525351.1 type IV pilus secretin PilQ family protein Machinery gene
  M2042_RS14935 (HLBS07_28520) pilP 3104806..3105321 (-) 516 WP_005382766.1 pilus assembly protein PilP Machinery gene
  M2042_RS14940 (HLBS07_28530) pilO 3105311..3105904 (-) 594 WP_064354357.1 type 4a pilus biogenesis protein PilO Machinery gene
  M2042_RS14945 (HLBS07_28540) pilN 3105897..3106472 (-) 576 WP_005381323.1 PilN domain-containing protein Machinery gene
  M2042_RS14950 (HLBS07_28550) pilM 3106456..3107472 (-) 1017 WP_005381324.1 type IV pilus assembly protein PilM Machinery gene
  M2042_RS14955 (HLBS07_28560) - 3107617..3110178 (+) 2562 WP_025768081.1 PBP1A family penicillin-binding protein -
  M2042_RS14960 (HLBS07_28570) oxyR 3110231..3111142 (-) 912 WP_005381326.1 DNA-binding transcriptional regulator OxyR -
  M2042_RS14965 (HLBS07_28580) - 3111312..3112040 (+) 729 WP_005382781.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37581.92 Da        Isoelectric Point: 7.2224

>NTDB_id=81554 M2042_RS14950 WP_005381324.1 3106456..3107472(-) (pilM) [Vibrio alginolyticus strain HLBS-07]
MDKLIVTGIDIGHNSLKAVVLKPTGNNYALLGYKEILLKEGIVAENNTINHQEIVKTLKQIKKELPFGAKKVAISVPDNS
VISKKLQIEQNLEESEVEFAVIQAFSHQSPFPVEELSLDFVRLRAEGGQSGSDSYQVFATRKDVVEGRVDALQKSGLKPV
LVDVHSQSLGHIWKLTADRFPDKNKYCLLDIGTLSSSFTMFTEQGELFHKEFAYGTRGANSASPVDFLSDDAQEKTELFN
RQIVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSQQLSLECELLNPLGLFEMKVSKRKRRAVDWQHFSTAA
GLAVRGIHWLGGARALSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=81554 M2042_RS14950 WP_005381324.1 3106456..3107472(-) (pilM) [Vibrio alginolyticus strain HLBS-07]
ATGGATAAGCTAATCGTTACCGGCATCGATATAGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCACAGGCAACAA
TTACGCTCTGTTGGGTTACAAAGAAATATTACTAAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTCAAGCAGATAAAAAAAGAACTGCCTTTTGGCGCTAAAAAAGTTGCGATTTCAGTTCCTGATAACTCG
GTAATTAGCAAGAAACTGCAAATAGAACAAAATCTTGAAGAGAGTGAAGTTGAGTTTGCAGTGATACAAGCCTTTTCTCA
TCAATCGCCATTCCCTGTCGAAGAGCTGAGTTTAGACTTCGTTCGTCTTCGCGCTGAGGGCGGGCAGAGTGGCAGTGATA
GCTATCAAGTGTTTGCGACAAGAAAAGATGTGGTAGAAGGCCGAGTCGATGCATTACAAAAGTCCGGACTAAAACCTGTT
TTAGTGGACGTGCATTCGCAAAGCCTTGGTCATATCTGGAAGCTCACCGCCGATCGCTTTCCTGACAAAAACAAATACTG
CTTACTCGATATTGGCACTTTATCGAGCTCCTTCACCATGTTTACCGAACAAGGTGAACTGTTTCACAAAGAGTTTGCTT
ACGGCACACGCGGAGCAAACAGCGCTTCTCCAGTCGATTTTCTGTCCGATGATGCTCAGGAGAAAACAGAGCTGTTTAAC
CGTCAAATCGTAGAGCGAGTAAAGCGCCAGATGCAGCTCTATACCTCAATCAATGGCTCACAAAACATCAAGGGTATCTG
GTTATCAGGGGAAGGGGCCTCCACTCCCATGCTCGCAGAGGAGTTGTCTCAACAATTGTCGTTAGAGTGTGAATTGCTAA
ATCCGCTAGGGCTATTTGAGATGAAGGTGTCTAAGCGTAAGCGTCGTGCTGTGGATTGGCAGCATTTCTCAACAGCAGCC
GGACTGGCCGTTCGTGGCATTCATTGGCTAGGAGGCGCCCGTGCTTTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

83.136

100

0.831
  pilM Vibrio cholerae strain A1552

50.746

99.112

0.503

Multiple sequence alignment