Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   RZV12_RS04250 Genome accession   NZ_CP136552
Coordinates   892808..893248 (-) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli O104:H4 strain rpoS_ATGtoATA     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 887808..898248
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RZV12_RS04225 coaE 888298..888918 (-) 621 WP_001269527.1 dephospho-CoA kinase -
  RZV12_RS04230 - 888943..888987 (+) 45 WP_120795372.1 protein YacM -
  RZV12_RS04235 guaC 889143..890186 (+) 1044 WP_001217338.1 GMP reductase -
  RZV12_RS04240 hofC 890221..891423 (-) 1203 WP_000157234.1 protein transport protein HofC -
  RZV12_RS04245 pilB 891413..892798 (-) 1386 WP_001025173.1 type II secretion system protein GspE Machinery gene
  RZV12_RS04250 pilA 892808..893248 (-) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  RZV12_RS04255 nadC 893451..894344 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  RZV12_RS04260 ampD 894432..894983 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  RZV12_RS04265 ampE 894980..895834 (+) 855 WP_000172006.1 beta-lactamase regulator AmpE -
  RZV12_RS04270 aroP 895877..897250 (-) 1374 WP_000969914.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=815023 RZV12_RS04250 WP_000360895.1 892808..893248(-) (pilA) [Escherichia coli O104:H4 strain rpoS_ATGtoATA]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=815023 RZV12_RS04250 WP_000360895.1 892808..893248(-) (pilA) [Escherichia coli O104:H4 strain rpoS_ATGtoATA]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363