Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QBS18_RS17690 Genome accession   NZ_CP121856
Coordinates   3771106..3772218 (-) Length   370 a.a.
NCBI ID   WP_279507722.1    Uniprot ID   -
Organism   Aeromonas veronii strain LOID 16013     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3766106..3777218
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QBS18_RS17660 - 3766559..3767566 (-) 1008 WP_005340374.1 Fe(3+) ABC transporter substrate-binding protein -
  QBS18_RS17665 glnK 3767825..3768163 (-) 339 WP_005341406.1 P-II family nitrogen regulator -
  QBS18_RS17670 arfB 3768365..3768781 (+) 417 WP_111806077.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  QBS18_RS17675 - 3768814..3769305 (+) 492 WP_279499021.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  QBS18_RS17680 - 3769665..3770090 (-) 426 WP_005340360.1 hypothetical protein -
  QBS18_RS17685 yaaA 3770285..3771058 (-) 774 WP_279499020.1 peroxide stress protein YaaA -
  QBS18_RS17690 pilU 3771106..3772218 (-) 1113 WP_279507722.1 type IVa pilus ATPase TapU Machinery gene
  QBS18_RS17695 pilT 3772247..3773281 (-) 1035 WP_005340354.1 type IVa pilus ATPase TapT Machinery gene
  QBS18_RS17700 - 3773321..3774022 (+) 702 WP_279499019.1 YggS family pyridoxal phosphate-dependent enzyme -
  QBS18_RS17705 proC 3774165..3774989 (+) 825 WP_279499018.1 pyrroline-5-carboxylate reductase -
  QBS18_RS17710 - 3775001..3775552 (+) 552 WP_005341416.1 YggT family protein -
  QBS18_RS17715 yggU 3775552..3775854 (+) 303 WP_120414749.1 DUF167 family protein YggU -
  QBS18_RS17720 - 3775898..3776317 (+) 420 WP_120414748.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41133.17 Da        Isoelectric Point: 6.5043

>NTDB_id=814833 QBS18_RS17690 WP_279507722.1 3771106..3772218(-) (pilU) [Aeromonas veronii strain LOID 16013]
MNLDDLLSELVERKGSDLFVTVGSPPTLKVNGHLVSLGGGEALDKKGALTLVRDTLSSDHFERYIRTKEANYAIYREALG
RFRVSAFWQQELPGMVVRRIETRIPTFEDLQLPKILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGYRNQHADGHILTVE
DPVEFVHQHGRSLVTQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDR
ILHLVPQDKHRQFLFDLSFNLKAIVAQQLVPSMDGKRRCAAFEILLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQ
ALFSLFCAGQIGYSEALAHADSANDLRLLIKLSGRERLGTGTLDNVTLDE

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=814833 QBS18_RS17690 WP_279507722.1 3771106..3772218(-) (pilU) [Aeromonas veronii strain LOID 16013]
ATGAATCTGGATGATCTGCTGAGCGAGCTGGTCGAGCGAAAGGGATCAGATCTGTTTGTGACGGTGGGCTCGCCGCCCAC
CCTCAAGGTGAATGGCCATCTGGTGTCGCTGGGGGGGGGGGAAGCGCTCGACAAGAAGGGGGCGCTGACGCTGGTCAGGG
ATACCCTCAGCAGCGATCACTTCGAGCGCTATATCCGCACCAAGGAGGCCAACTACGCGATCTATCGCGAAGCGCTTGGC
CGTTTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCTGGCATGGTGGTGCGGCGTATCGAGACCCGCATTCCCACCTT
TGAAGATCTGCAGCTACCCAAAATTCTGCAAGAGGTGGCGATGGCCAAGCGGGGGCTGGTGCTGTTTGTTGGCGCCACCG
GGGCGGGCAAATCGACTACCCAGGCGGCGATGATCGGCTATCGCAACCAGCATGCCGATGGTCACATTCTGACGGTGGAA
GACCCGGTGGAGTTCGTCCATCAGCATGGTCGCAGTCTGGTGACCCAGCGAGAGGTGGGGATCGACACCGAGTCATTCGA
TGTGGCGCTGAAAAGTTCGCTGCGTCAGGCGCCGGACGTGATCCTGATCGGCGAGATCCGGAGTCAGGAGACCATGGAGT
TTGCCCTGCAGTTCGCCGAGACGGGACACCTTTGTCTCGCCACTTTGCATGCCAACAATGCCAACCAGGCGCTGGATCGC
ATCCTCCATCTGGTGCCGCAGGACAAACACCGCCAGTTCCTGTTCGATCTCTCTTTCAACCTCAAGGCCATCGTCGCCCA
GCAGCTGGTGCCGAGTATGGATGGCAAGCGGCGCTGCGCTGCGTTCGAGATCCTGCTCAATACCCCGCTCATCACTGACA
TTATCCGCAAGGGAGAGATGCATCGCCTCAAGGAGGTGATGACCAAATCCACCGAGCTGGGTATGCAGACCTTCGATCAA
GCGCTCTTCAGCCTGTTCTGCGCCGGCCAGATTGGCTACAGTGAGGCACTCGCCCACGCCGACTCGGCCAACGACCTGCG
ACTGCTGATCAAGCTCTCCGGTCGCGAGCGGCTCGGTACCGGCACGCTGGACAATGTGACGCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.163

99.73

0.6
  pilU Pseudomonas stutzeri DSM 10701

60.227

95.135

0.573
  pilU Acinetobacter baylyi ADP1

55.707

99.459

0.554
  pilT Legionella pneumophila strain ERS1305867

40.698

92.973

0.378
  pilT Legionella pneumophila strain Lp02

40.698

92.973

0.378
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.706

91.892

0.365
  pilT Acinetobacter baylyi ADP1

39.181

92.432

0.362