Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   KI219_RS04965 Genome accession   NZ_AP023089
Coordinates   1025099..1026163 (-) Length   354 a.a.
NCBI ID   WP_039072848.1    Uniprot ID   -
Organism   Bacillus paralicheniformis strain RSC-2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1020099..1031163
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KI219_RS21600 (RSC2_01044) - 1020594..1020824 (-) 231 WP_244834609.1 hypothetical protein -
  KI219_RS21605 (RSC2_01045) tapA 1020893..1021321 (-) 429 WP_244834611.1 amyloid fiber anchoring/assembly protein TapA -
  KI219_RS04925 (RSC2_01046) - 1021599..1021919 (+) 321 WP_023855188.1 YqzG/YhdC family protein -
  KI219_RS04930 (RSC2_01047) - 1021949..1022131 (-) 183 WP_020452171.1 YqzE family protein -
  KI219_RS04935 (RSC2_01048) comGG 1022220..1022585 (-) 366 WP_025811163.1 competence type IV pilus minor pilin ComGG -
  KI219_RS04940 (RSC2_01049) comGF 1022597..1023085 (-) 489 WP_224067282.1 competence type IV pilus minor pilin ComGF -
  KI219_RS04945 (RSC2_01050) comGE 1022994..1023341 (-) 348 WP_023855191.1 competence type IV pilus minor pilin ComGE -
  KI219_RS04950 (RSC2_01051) comGD 1023325..1023762 (-) 438 WP_039072845.1 competence type IV pilus minor pilin ComGD -
  KI219_RS04955 (RSC2_01052) comGC 1023768..1024061 (-) 294 WP_039072846.1 competence type IV pilus major pilin ComGC Machinery gene
  KI219_RS04960 (RSC2_01053) comGB 1024075..1025109 (-) 1035 WP_039072847.1 competence type IV pilus assembly protein ComGB Machinery gene
  KI219_RS04965 (RSC2_01054) comGA 1025099..1026163 (-) 1065 WP_039072848.1 competence type IV pilus ATPase ComGA Machinery gene
  KI219_RS04970 (RSC2_01055) - 1026321..1027160 (-) 840 WP_039072849.1 STAS domain-containing protein -
  KI219_RS04980 (RSC2_01056) - 1027402..1027779 (-) 378 WP_003183482.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  KI219_RS04985 (RSC2_01057) - 1027990..1028235 (+) 246 WP_020452180.1 DUF2626 domain-containing protein -
  KI219_RS04990 (RSC2_01058) - 1028271..1028909 (-) 639 WP_025811156.1 MBL fold metallo-hydrolase -
  KI219_RS04995 (RSC2_01059) - 1029065..1029238 (+) 174 WP_003183486.1 DUF2759 domain-containing protein -
  KI219_RS05000 (RSC2_01060) - 1029299..1029613 (-) 315 WP_020452182.1 MTH1187 family thiamine-binding protein -
  KI219_RS05005 (RSC2_01061) - 1029629..1030738 (-) 1110 WP_039072850.1 hypothetical protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 39838.33 Da        Isoelectric Point: 9.1063

>NTDB_id=81343 KI219_RS04965 WP_039072848.1 1025099..1026163(-) (comGA) [Bacillus paralicheniformis strain RSC-2]
MYTIESLSGRLIDEAYRMKASDIHIVPGEKEAVVRFRIDDELFQKERLTRNECSRLISHFKFLSSMDIGERRLPQSGALT
LYINRQPVHLRMSTLPTIHDESLVIRLLPKMSSKPLTKLSLFPSATFKLLSFLKHSHGLILFTGPTGSGKTTTLYSLIEY
AKRHFKRNIITLEDPVESRSENILQVQVNEKAGMTYSAGLKAVLRHDPDMIILGEIRDAETAQIAVRAALTGHLVLSSMH
AKNAKGAIYRLLEFGVDMTEIEQTLVAISAQRLVGLVCPFCGDNCSLYCRLSRPVRRASVFELLYGKSLNLCIEEAKGRC
GDIKTETLKTLIQKGIALGYLPSNTYERWIGHED

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=81343 KI219_RS04965 WP_039072848.1 1025099..1026163(-) (comGA) [Bacillus paralicheniformis strain RSC-2]
TTGTACACGATTGAATCATTAAGCGGAAGATTAATTGACGAGGCATATAGAATGAAGGCGTCAGACATTCATATTGTTCC
CGGTGAAAAAGAGGCGGTGGTTCGCTTCAGGATTGATGATGAACTTTTTCAAAAAGAAAGATTAACGAGAAATGAGTGCT
CAAGGCTCATTTCTCACTTTAAATTCCTTTCTTCGATGGATATTGGGGAAAGACGGCTGCCGCAAAGCGGAGCTTTGACT
CTTTATATCAACCGTCAGCCCGTTCATTTAAGAATGTCCACTCTGCCCACCATCCATGATGAAAGCTTGGTCATCAGGCT
TCTTCCCAAGATGAGCAGCAAGCCGCTCACAAAGCTCTCATTGTTTCCGAGTGCAACATTCAAGCTGCTGTCTTTTCTGA
AGCATTCCCACGGATTAATTCTGTTCACCGGTCCGACAGGCTCGGGGAAAACGACAACACTCTATTCTTTAATCGAATAC
GCTAAGCGGCATTTTAAGCGAAATATCATTACCTTGGAAGACCCTGTTGAATCAAGAAGCGAAAACATTTTGCAAGTGCA
AGTAAACGAAAAAGCTGGCATGACTTATTCTGCGGGGTTGAAGGCGGTGCTTCGCCACGATCCGGACATGATCATCCTCG
GGGAAATTCGCGATGCCGAAACAGCCCAAATCGCAGTCAGGGCAGCTTTGACAGGACACCTTGTTTTATCGAGCATGCAT
GCGAAAAATGCCAAAGGCGCAATATACAGGCTGCTCGAGTTCGGCGTTGATATGACAGAAATTGAACAAACACTGGTCGC
AATCAGCGCCCAGCGCCTCGTCGGTCTCGTCTGCCCGTTTTGCGGCGACAACTGCTCTTTATATTGCAGATTGTCGAGGC
CTGTAAGAAGAGCAAGCGTCTTCGAGCTTTTGTACGGAAAAAGCCTCAATCTCTGCATCGAAGAAGCAAAAGGCAGGTGC
GGTGACATAAAAACAGAAACATTGAAAACGCTGATTCAGAAGGGGATAGCACTCGGATATCTGCCGTCGAATACTTATGA
ACGCTGGATAGGCCATGAAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

67.797

100

0.678
  pilB Vibrio cholerae strain A1552

38.671

93.503

0.362

Multiple sequence alignment