Detailed information NTG0810370

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: P8T35_RS03135
• Genome accession: NZ_CP121139
• Coordinates: 675396..676196 (-)
• Length: 266 a.a.
• NCBI ID: WP_032414527.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain UHD-4_PH
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 670396..681196

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P8T35_RS03115 (P8T35_03115)	dksA	670976..671431 (-)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-
P8T35_RS03120 (P8T35_03120)	sfsA	671594..672310 (-)	717	WP_004177390.1	DNA/RNA nuclease SfsA	-
P8T35_RS03125 (P8T35_03125)	thpR	672310..672846 (-)	537	WP_032414524.1	RNA 2',3'-cyclic phosphodiesterase	-
P8T35_RS03130 (P8T35_03130)	hrpB	672919..675348 (+)	2430	WP_032414526.1	ATP-dependent helicase HrpB	-
P8T35_RS03135 (P8T35_03135)	pilD	675396..676196 (-)	801	WP_032414527.1	A24 family peptidase	Machinery gene
P8T35_RS03140 (P8T35_03140)	-	676193..676939 (-)	747	WP_009309263.1	type II secretion system protein N	-
P8T35_RS03145 (P8T35_03145)	-	676929..677414 (-)	486	WP_004145896.1	type II secretion system protein M	-
P8T35_RS03150 (P8T35_03150)	gspL	677407..678600 (-)	1194	WP_004191957.1	type II secretion system protein GspL	-
P8T35_RS03155 (P8T35_03155)	gspK	678587..679573 (-)	987	WP_077254427.1	type II secretion system minor pseudopilin GspK	-
P8T35_RS03160 (P8T35_03160)	gspJ	679570..680166 (-)	597	WP_004145893.1	type II secretion system minor pseudopilin GspJ	-
P8T35_RS03165 (P8T35_03165)	gspI	680163..680528 (-)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
P8T35_RS03170 (P8T35_03170)	gspH	680525..681040 (-)	516	WP_032414529.1	type II secretion system minor pseudopilin GspH	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29419.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=810370 P8T35_RS03135 WP_032414527.1 675396..676196(-) (pilD) [Klebsiella pneumoniae strain UHD-4_PH]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPISWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMI

Nucleotide

• Length: 801 bp

>NTDB_id=810370 P8T35_RS03135 WP_032414527.1 675396..676196(-) (pilD) [Klebsiella pneumoniae strain UHD-4_PH]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCTCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAACTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGATCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.057	99.624	0.489
pilD	Vibrio campbellii strain DS40M4	46.545	100	0.481
pilD	Acinetobacter nosocomialis M2	46.388	98.872	0.459
pilD	Acinetobacter baumannii D1279779	45.627	98.872	0.451
pilD	Neisseria gonorrhoeae MS11	43.922	95.865	0.421