Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   P4829_RS05760 Genome accession   NZ_CP120846
Coordinates   1150844..1154548 (+) Length   1234 a.a.
NCBI ID   WP_063637347.1    Uniprot ID   -
Organism   Bacillus atrophaeus strain MBLB1156     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1145844..1159548
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P4829_RS05745 (P4829_05745) - 1146291..1146617 (-) 327 WP_081250920.1 four-helix bundle copper-binding protein -
  P4829_RS05750 (P4829_05750) - 1146714..1147151 (-) 438 WP_063637345.1 ferritin family protein -
  P4829_RS05755 (P4829_05755) addB 1147357..1150857 (+) 3501 WP_063637346.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  P4829_RS05760 (P4829_05760) addA 1150844..1154548 (+) 3705 WP_063637347.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  P4829_RS05765 (P4829_05765) sbcD 1154619..1155797 (+) 1179 WP_010788168.1 exonuclease subunit SbcD -
  P4829_RS05770 (P4829_05770) sbcC 1155794..1159183 (+) 3390 WP_063637348.1 exonuclease subunit SbcC -
  P4829_RS05775 (P4829_05775) - 1159200..1159502 (+) 303 WP_010788170.1 hypothetical protein -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 141490.03 Da        Isoelectric Point: 5.4650

>NTDB_id=808828 P4829_RS05760 WP_063637347.1 1150844..1154548(+) (addA) [Bacillus atrophaeus strain MBLB1156]
MQIPKPADSTWTDDQWNAIVSSGQDILVAAAAGSGKTAVLVERMIRKITAEENPIDVDRLLVVTFTNASAAEMKHRIAEA
LEKELVKRPGSLHIRRQLSLLNRASISTLHSFCLQVLRKYYYLIDLDPGFRIADQTEGELLGDEVLDELFEDEYAKGEKA
FFELVDRYTTDRHDLDLQHLVKRVYEFSRSHPDPKTWLEGFVHLYDASEKSTIEELPFYPYMKEDIAMVLSAVKEKLQRA
LELTKVPGGPAPRADNFLDDLAQTEDLIAHSSDFTELYKRVPAVSFKRAKAVKGDEFDQALTEEATDLRNGAKKLLEKLK
TDYFTRSPEQHLKSLSDMKPVIETLVQLVISYGKRFEAAKQEKSIIDFSDLEHYCLSILTAVNESGERKPSEAAAYYQEQ
FQEVLVDEYQDTNLVQEAILQLVTSGPEASGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTETGEGTGRKIDLNKNFRS
RADILDSTNFLFKQLMGGKVGEVDYDEQAELKLGASYPPNDQTETELLLIDRAEEKEASEETEELETVQLEAKAIAKQIR
KLVSSPYKVYDGKSKTHSNIRYRDIVILLRSMPWAPQIMEELKAQGIPVYANLTSGYFEAVEVSVAISALKVIDNPYQDI
PLASVLRSPIVGADENELSLIRMHNKKAPFYEAMKDYLAGGDRNDELYQKLNTFFELLQKWRSFSKNHSVSELIWEVYRD
SKYMDYVGGMPGGKQRQANLRVLYDRSRQYESTAFRGLFRFLRFIERMQERGDDLGTARAISEQEDVVRLMTIHSSKGLE
FPVVFAAGLGRNFNMMDLNKSYLLDKELGFGTKFIHPEWRISYPTLPLIAMKKKMRRELLSEELRVLYVALTRAKEKLFL
VGSCKNREKQLAKWQASASQPDWLLPDFERYQAKTYLDFIGPALIRHRDMGTDALQGLASQADISEHPARFNIQMVSAQD
LLDDDLEERQEEKSGRLDAIRRGEPVSGSFEFEEQARRRLSWRYPYHDVTKIRTKQSVSEIKRKKEYEDEYSGRSPIRPA
DGNMLYRRPAFMMKKGLTAAEKGTAMHTVMQHIPLTHAPSKEEAERIVKRLYEKELLTEDQQAVIDLEEIVQFFHTEIGE
KLIGAKWINREVPFSMALSAQDAFLDTESKDEPILVQGIIDCLYETDEGLFLLDYKTDRIEGKFQHGFEGAAPILKKRYE
TQIELYTKAVEQITKTNVKGRALYFFDGGHVLNV

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=808828 P4829_RS05760 WP_063637347.1 1150844..1154548(+) (addA) [Bacillus atrophaeus strain MBLB1156]
ATGCAAATTCCTAAACCGGCAGACAGCACCTGGACAGATGACCAGTGGAACGCCATTGTCTCATCCGGACAGGATATTCT
CGTTGCAGCGGCTGCTGGATCTGGAAAAACCGCTGTGCTTGTTGAGAGAATGATCCGCAAAATTACCGCGGAGGAAAATC
CGATAGATGTGGACCGTCTTTTGGTCGTGACATTTACGAACGCCTCTGCGGCTGAAATGAAGCACCGGATCGCGGAGGCT
TTGGAGAAAGAGCTCGTGAAACGTCCCGGCTCTCTTCATATCAGGCGGCAGCTGTCTCTGTTGAACCGGGCCAGCATTTC
AACCCTTCATTCGTTCTGCTTGCAGGTGCTCAGAAAATATTATTATCTCATTGATCTTGATCCGGGCTTTCGGATTGCCG
ATCAGACAGAAGGCGAGCTGCTCGGGGACGAGGTCCTTGATGAACTGTTTGAAGACGAATACGCGAAGGGTGAAAAAGCG
TTTTTCGAACTGGTGGACCGGTATACGACAGACCGGCACGACTTAGACCTTCAGCACCTCGTGAAACGGGTGTATGAGTT
TTCCCGTTCCCATCCCGATCCAAAAACGTGGCTGGAAGGTTTCGTTCATCTTTACGATGCTTCAGAAAAAAGCACGATTG
AAGAACTGCCGTTTTATCCATATATGAAAGAAGACATTGCGATGGTGCTGAGTGCTGTGAAAGAAAAGCTTCAGCGGGCG
CTTGAGCTGACAAAAGTGCCGGGCGGCCCTGCGCCGCGCGCTGATAATTTTCTGGATGATCTTGCACAGACAGAGGATCT
GATCGCACATTCGTCGGATTTCACTGAATTATACAAGCGGGTTCCCGCAGTCTCCTTTAAGCGTGCCAAAGCGGTCAAAG
GAGATGAATTTGACCAGGCGCTCACAGAAGAAGCGACGGATTTACGGAACGGCGCGAAAAAACTGCTGGAAAAACTAAAA
ACGGACTATTTCACAAGAAGCCCCGAGCAGCATCTGAAGAGCCTGTCGGATATGAAGCCTGTGATTGAAACGCTCGTCCA
GCTTGTCATCAGCTACGGAAAACGGTTTGAAGCCGCCAAACAAGAGAAATCAATTATTGATTTCTCTGACTTGGAGCATT
ACTGCTTGTCTATCCTGACAGCCGTGAATGAGTCGGGGGAGCGCAAGCCGAGTGAGGCGGCGGCTTACTATCAAGAGCAG
TTCCAAGAGGTGCTGGTTGACGAATATCAAGATACGAATCTTGTGCAGGAAGCCATCCTGCAGCTTGTCACAAGCGGACC
TGAAGCTTCAGGAAACTTGTTTATGGTAGGGGACGTCAAACAGTCCATCTACCGATTCAGGCTTGCCGAACCGCTTCTTT
TCCTTTCTAAATATAAACGATTTACCGAAACGGGAGAGGGGACAGGCCGGAAAATTGATTTGAACAAAAACTTTCGAAGC
CGGGCGGATATTTTAGACAGCACGAACTTTCTGTTTAAGCAGCTCATGGGCGGCAAGGTCGGCGAGGTTGATTATGATGA
GCAGGCGGAATTGAAGCTAGGAGCCTCTTACCCTCCGAATGACCAGACTGAAACGGAGCTGTTGCTGATTGACAGAGCGG
AGGAAAAAGAGGCGAGTGAAGAAACAGAAGAGCTCGAAACCGTTCAGCTTGAAGCAAAAGCGATTGCAAAGCAGATTCGA
AAGCTCGTATCCTCTCCTTACAAAGTCTATGATGGAAAATCGAAAACACACAGCAATATCCGCTACCGCGATATCGTGAT
TTTACTGCGTTCCATGCCGTGGGCGCCGCAAATCATGGAAGAACTGAAAGCTCAAGGCATACCCGTCTATGCCAATTTGA
CATCAGGCTATTTTGAAGCGGTCGAGGTGTCTGTTGCGATTTCCGCTTTAAAAGTGATTGATAATCCGTACCAAGATATT
CCTCTCGCTTCCGTGCTGCGCTCGCCGATTGTCGGCGCGGATGAAAATGAACTTTCGCTGATTCGGATGCATAATAAAAA
AGCGCCTTTCTATGAAGCGATGAAGGACTATCTTGCAGGCGGGGACCGAAATGACGAGCTATATCAAAAGCTGAACACGT
TTTTTGAGCTTTTGCAAAAATGGCGCTCTTTTTCGAAAAACCATTCAGTTTCCGAGCTGATATGGGAAGTTTATCGTGAC
AGCAAATATATGGACTATGTCGGCGGTATGCCGGGCGGAAAACAGCGGCAGGCCAATTTGCGCGTTCTCTATGACCGGTC
CCGTCAGTATGAATCGACGGCATTTCGCGGTTTATTTCGTTTCTTGCGGTTTATAGAACGTATGCAGGAACGGGGCGATG
ACCTCGGTACAGCGAGAGCGATCAGCGAGCAGGAGGACGTGGTCCGCCTGATGACGATTCACAGCAGCAAGGGGCTTGAA
TTCCCTGTCGTATTCGCTGCAGGACTTGGACGGAATTTTAATATGATGGATTTGAATAAATCATATCTGCTGGACAAAGA
GCTTGGTTTCGGCACAAAGTTTATTCACCCCGAATGGCGCATCAGCTATCCGACACTTCCTCTCATCGCCATGAAGAAAA
AAATGCGCCGGGAGCTGCTGTCAGAAGAGCTGCGTGTGCTATACGTGGCTTTAACAAGAGCGAAGGAAAAGCTGTTTCTT
GTCGGTTCCTGCAAGAATCGTGAGAAGCAGCTGGCGAAATGGCAGGCTTCAGCGTCTCAGCCAGACTGGCTTCTCCCGGA
TTTTGAGCGGTATCAGGCGAAAACGTATTTGGATTTCATCGGTCCCGCATTGATTCGTCACAGAGACATGGGGACAGATG
CTTTACAAGGGCTTGCGAGTCAAGCTGACATATCAGAACACCCTGCCCGGTTTAACATCCAAATGGTCAGCGCCCAAGAT
TTATTGGATGATGATCTGGAAGAAAGACAAGAGGAAAAGAGCGGCCGTCTTGACGCCATCCGCCGCGGTGAGCCTGTGTC
CGGTTCGTTTGAATTCGAAGAACAAGCGCGGCGACGCCTGTCATGGCGATACCCTTATCACGACGTGACAAAAATTCGCA
CGAAGCAGTCTGTTTCCGAGATCAAGAGAAAGAAAGAATACGAGGACGAATACAGCGGCCGCTCTCCCATCAGACCGGCT
GACGGAAACATGTTGTACAGGCGCCCTGCGTTTATGATGAAAAAAGGGCTGACTGCGGCTGAAAAAGGAACGGCGATGCA
CACCGTTATGCAGCATATCCCGCTGACACATGCGCCGTCAAAAGAGGAAGCGGAACGTATTGTCAAACGTCTTTATGAAA
AAGAGCTGCTCACCGAAGACCAGCAAGCCGTTATTGACCTAGAGGAAATCGTGCAATTTTTCCATACTGAAATCGGCGAA
AAGCTGATCGGCGCAAAATGGATTAACCGCGAAGTGCCGTTTAGCATGGCGCTTTCAGCACAAGATGCGTTTCTGGACAC
TGAATCTAAAGATGAGCCTATATTGGTGCAAGGGATTATTGACTGTCTGTATGAGACCGATGAAGGGCTTTTCTTGCTGG
ATTACAAAACGGACCGAATCGAAGGGAAATTCCAGCACGGATTTGAGGGGGCGGCTCCTATTCTTAAAAAACGGTACGAA
ACTCAAATCGAGTTATACACAAAAGCTGTAGAACAAATAACGAAAACCAATGTGAAGGGACGTGCACTCTACTTCTTCGA
CGGAGGACACGTTTTGAACGTATAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1055-1226)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)

UvrD-like helicase C-terminal domain (UvrD_C)

(472-881)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

86.629

100

0.866