Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   P4829_RS05755 Genome accession   NZ_CP120846
Coordinates   1147357..1150857 (+) Length   1166 a.a.
NCBI ID   WP_063637346.1    Uniprot ID   -
Organism   Bacillus atrophaeus strain MBLB1156     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1142357..1155857
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P4829_RS05730 (P4829_05730) - 1142752..1144245 (+) 1494 WP_155729071.1 monooxygenase -
  P4829_RS05735 (P4829_05735) - 1144329..1144859 (+) 531 WP_003327686.1 MarR family transcriptional regulator -
  P4829_RS05740 (P4829_05740) - 1144909..1146057 (-) 1149 WP_063637343.1 AbrB family transcriptional regulator -
  P4829_RS05745 (P4829_05745) - 1146291..1146617 (-) 327 WP_081250920.1 four-helix bundle copper-binding protein -
  P4829_RS05750 (P4829_05750) - 1146714..1147151 (-) 438 WP_063637345.1 ferritin family protein -
  P4829_RS05755 (P4829_05755) addB 1147357..1150857 (+) 3501 WP_063637346.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  P4829_RS05760 (P4829_05760) addA 1150844..1154548 (+) 3705 WP_063637347.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  P4829_RS05765 (P4829_05765) sbcD 1154619..1155797 (+) 1179 WP_010788168.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1166 a.a.        Molecular weight: 134394.22 Da        Isoelectric Point: 5.1681

>NTDB_id=808827 P4829_RS05755 WP_063637346.1 1147357..1150857(+) (addB) [Bacillus atrophaeus strain MBLB1156]
MGAEFLVGRSGSGKTKLIIDSIQDELRRNPFGKPIIFLVPDQMTFLMEYELAKTPGIGGMIRAQVFSFSRLAWRVLQHTG
GMNRPFVTSTGIQMLLRKLIEEHKQEFKVYQKASDKSGFTEQVERMLTEFKRYCLEPEDIRRMIESGTATDYRGERVLTD
KLHDLYILYQQMEKSLENQYLHSEDYLTLLAEQIPYAEDLKGAHVYVDGFYQFTPQEFRVLTQLMAQAEHVTFSLTADKP
SHVQAPDELDLFRMTGKTYYQLHQAASELHLDITNRELTETKRYQQTPELAHLEAMYDARPAVPFLEKQEAFTVMQAANR
RTELEGISREIHALVRDKGLRYKDMAILARQPEDYKDMVKEVFADYEIPYFIDGKASMLNHPLVEFIRSSLDVIKGNWRY
EAVFRCVKTELLFPLDRPKNIIREQVDQLENYCIAYGIKGDRWTRGDRFVYRRFVSLDDDFAQTDQEIEMENMLNDTRDW
IVPPLFRLQKRMKQAKTVQDMAEALYLYLEETDIPLKLDQERQRAEEDGRIIEAQQHNQAWDAVIQLLEEFVEMMGEDEI
SLSLFQQMMETGAESLKFALIPPALDQVFVGNMDLSRMYGTRCTFVIGANDGVLPARPEDDGVLSDDDREWLKTIGVELS
SGGRERLLDEHFLIYMALSSPSDRLYVSYPIADAEGKTLLPSTVVKRLTELFPEHQERLLTNEPEQVSDCEQLLYLVNKS
VAQSYTASQFRLWLREYDISDVWWSAYNVLMNEPDRLNSKKLFSSLFFRNEVKPLERAVSRQLYGEHIHGSVSRMETFNA
CPFSHFASHGLHLKERQFFKLEAPDIGQLFHSSLKLISDRLREQKLDWRDLTKDQCNKFSYEAVERLAPKLQKEILLSSN
RHYYVKEKLQKIVARVSGILSEHAKASGFAPVGLELGFGGKGPLPPLTFTLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLSEVYYGLALQMLTYLDLSITYSADWLGMKATPAGVLYFHIHDPMIQASVPLGLDEIEQEIFKKFKMKGL
LLGDQEAIRLMDTTLQEGRSNIINAGLKKDGSLRSDSAAVGEEEFHLLTQHVRRTFQEAGEQITDGCVSIEPYKLKDKTP
CTYCSFKSVCQFDESLEENEYRTLKAEKDHTILDWLKKEGDGDANS

Nucleotide


Download         Length: 3501 bp        

>NTDB_id=808827 P4829_RS05755 WP_063637346.1 1147357..1150857(+) (addB) [Bacillus atrophaeus strain MBLB1156]
TTGGGAGCAGAGTTTTTAGTAGGCAGATCCGGGAGTGGAAAAACGAAGCTGATCATAGACAGCATTCAAGATGAGCTGCG
CCGGAATCCATTCGGGAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATATGAGCTTGCTAAAA
CGCCTGGTATAGGCGGAATGATACGCGCTCAAGTGTTCAGCTTTTCACGTTTGGCCTGGCGTGTTCTGCAGCATACGGGC
GGAATGAACCGGCCGTTTGTCACCAGCACGGGAATCCAAATGCTTTTGAGAAAGCTCATTGAAGAGCATAAACAGGAGTT
TAAAGTCTATCAAAAAGCGAGTGACAAAAGCGGGTTTACCGAACAGGTAGAACGGATGCTGACGGAATTTAAACGGTACT
GCCTTGAGCCTGAAGATATCCGCCGCATGATTGAAAGCGGAACTGCCACAGACTATCGCGGCGAGCGGGTGTTAACTGAT
AAGCTGCATGATTTGTATATTTTATATCAGCAGATGGAAAAGAGCCTTGAAAACCAGTATCTTCATTCAGAGGATTATTT
AACGCTGCTGGCGGAACAGATCCCGTATGCGGAAGATTTGAAAGGGGCGCATGTCTATGTAGACGGCTTTTATCAGTTTA
CTCCGCAGGAATTCAGAGTGCTTACGCAGCTTATGGCACAGGCTGAGCATGTGACGTTTTCACTGACGGCGGACAAGCCG
TCCCATGTGCAGGCGCCTGATGAGCTGGATCTGTTCAGAATGACAGGCAAAACATATTATCAGCTCCATCAAGCAGCTTC
GGAGCTTCATTTGGACATCACAAACAGAGAACTGACCGAGACGAAACGATATCAGCAAACACCGGAGCTTGCCCATTTAG
AAGCAATGTATGACGCGCGCCCTGCTGTTCCGTTTTTGGAAAAGCAAGAAGCCTTTACCGTGATGCAGGCTGCGAACAGG
CGGACAGAGCTGGAAGGAATTTCCCGTGAAATTCATGCTCTTGTAAGAGATAAGGGATTACGCTACAAGGATATGGCAAT
TCTCGCCCGCCAGCCGGAAGATTATAAGGATATGGTTAAAGAAGTATTTGCAGATTATGAAATTCCTTACTTTATAGATG
GAAAGGCATCCATGCTGAACCATCCGCTTGTTGAGTTTATCCGTTCAAGCCTTGATGTCATAAAGGGAAATTGGAGATAT
GAAGCGGTGTTTCGCTGTGTGAAAACCGAACTGTTATTCCCGCTTGACCGTCCGAAGAACATCATAAGGGAGCAGGTCGA
TCAACTGGAAAACTATTGTATCGCTTACGGAATTAAAGGCGACCGATGGACGAGAGGCGACCGTTTTGTTTACAGGCGGT
TTGTTTCACTGGATGATGATTTTGCGCAGACAGATCAAGAGATTGAAATGGAAAACATGCTGAATGACACAAGAGACTGG
ATTGTTCCGCCGCTTTTCCGTCTTCAAAAACGGATGAAACAAGCAAAAACGGTCCAAGACATGGCGGAGGCTCTTTATCT
TTATTTAGAAGAAACGGATATTCCGTTAAAGCTGGACCAAGAAAGACAGCGCGCGGAAGAGGACGGGAGAATTATCGAAG
CGCAGCAGCATAATCAGGCTTGGGACGCTGTTATACAGCTTCTCGAGGAATTTGTGGAAATGATGGGCGAAGACGAAATT
TCTCTGTCTCTTTTTCAGCAAATGATGGAGACGGGTGCGGAATCGTTAAAATTCGCTCTGATTCCGCCTGCACTCGATCA
AGTCTTTGTCGGCAATATGGATTTATCGAGAATGTACGGTACACGCTGCACCTTTGTTATCGGTGCGAACGACGGCGTAC
TTCCGGCGCGGCCTGAGGATGACGGGGTGCTGTCTGATGACGACAGGGAATGGCTGAAAACCATTGGAGTCGAGCTGTCA
TCCGGAGGGCGCGAACGTCTGCTTGATGAGCACTTTCTCATATATATGGCGCTTTCCAGTCCGTCTGACCGCCTTTATGT
ATCCTATCCGATCGCAGACGCCGAAGGTAAAACGCTTTTGCCGTCAACTGTGGTCAAACGGCTTACAGAACTGTTTCCGG
AGCATCAGGAAAGGCTTTTGACCAATGAACCGGAACAGGTCAGCGACTGCGAACAGCTCTTGTATCTCGTCAATAAAAGC
GTGGCGCAATCGTATACGGCAAGCCAATTCAGGTTGTGGTTAAGGGAGTATGACATCAGTGATGTATGGTGGAGCGCGTA
TAATGTATTGATGAATGAACCGGACAGGCTGAACTCGAAAAAACTTTTCTCCAGCCTGTTTTTCAGAAATGAAGTCAAAC
CGCTTGAACGCGCTGTATCGAGACAGCTGTACGGCGAGCATATTCATGGAAGTGTGTCCAGAATGGAGACCTTTAACGCT
TGTCCGTTCTCCCATTTTGCGTCCCACGGCTTACATCTGAAAGAACGGCAATTTTTCAAGCTGGAAGCGCCGGATATCGG
ACAGCTGTTCCATTCCAGTCTGAAGCTGATTTCAGACCGGCTTCGTGAACAAAAGCTGGATTGGCGCGATTTAACAAAAG
ACCAATGTAATAAGTTCTCTTACGAGGCGGTGGAGCGGCTCGCGCCGAAGCTCCAAAAAGAAATTCTGCTTAGCTCAAAC
AGACATTATTATGTCAAGGAAAAGCTGCAAAAAATTGTGGCGCGCGTGTCCGGCATTTTAAGCGAGCATGCGAAGGCGAG
TGGGTTTGCACCGGTTGGGCTTGAGCTTGGTTTTGGCGGGAAAGGCCCGCTGCCGCCTCTTACATTCACATTAAAAAACG
GCTGCACGATGGAGCTTGTCGGACGCATTGACCGCGTAGATAAAGCGGAAAGTTCAAAAGGGCTGCTGCTGAGAATAGTG
GATTATAAATCCAGCGACAAGGGTCTTGATTTGTCAGAGGTATACTACGGCCTTGCCCTGCAAATGCTCACGTACCTTGA
TTTATCCATTACGTATTCGGCAGATTGGCTCGGCATGAAGGCTACACCGGCCGGCGTTCTTTATTTCCATATTCACGACC
CGATGATTCAAGCAAGCGTTCCGCTCGGGCTTGATGAAATAGAGCAGGAAATTTTTAAGAAATTTAAGATGAAAGGCCTG
CTGCTCGGCGATCAGGAAGCGATTCGATTAATGGATACGACGCTCCAAGAGGGAAGGTCAAATATCATTAATGCCGGGCT
TAAAAAAGACGGTTCATTAAGGTCTGATTCAGCGGCGGTCGGAGAAGAGGAATTTCATCTGCTGACACAGCACGTGCGGC
GGACCTTTCAGGAGGCCGGCGAACAAATCACCGACGGGTGTGTGTCGATTGAGCCGTATAAGCTGAAAGATAAAACACCC
TGCACATACTGCTCATTCAAATCAGTATGCCAGTTCGATGAATCATTGGAAGAAAACGAATATCGCACGCTGAAAGCGGA
AAAGGATCATACCATATTAGATTGGCTGAAAAAGGAGGGAGATGGCGATGCAAATTCCTAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(791-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

88.679

100

0.887