Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   P4829_RS00580 Genome accession   NZ_CP120846
Coordinates   105944..107323 (+) Length   459 a.a.
NCBI ID   WP_063639030.1    Uniprot ID   -
Organism   Bacillus atrophaeus strain MBLB1156     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 100944..112323
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P4829_RS00560 (P4829_00560) ctsR 101298..101762 (+) 465 WP_003328320.1 transcriptional regulator CtsR -
  P4829_RS00565 (P4829_00565) - 101776..102333 (+) 558 WP_003328319.1 UvrB/UvrC motif-containing protein -
  P4829_RS00570 (P4829_00570) - 102333..103424 (+) 1092 WP_010787416.1 protein arginine kinase -
  P4829_RS00575 (P4829_00575) clpC 103421..105859 (+) 2439 WP_063639031.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  P4829_RS00580 (P4829_00580) radA 105944..107323 (+) 1380 WP_063639030.1 DNA repair protein RadA Machinery gene
  P4829_RS00585 (P4829_00585) disA 107327..108409 (+) 1083 WP_010787418.1 DNA integrity scanning diadenylate cyclase DisA -
  P4829_RS00590 (P4829_00590) - 108530..109630 (+) 1101 WP_061570389.1 PIN/TRAM domain-containing protein -
  P4829_RS00595 (P4829_00595) ispD 109644..110342 (+) 699 WP_063639029.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  P4829_RS00600 (P4829_00600) ispF 110335..110811 (+) 477 WP_003328312.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49548.99 Da        Isoelectric Point: 8.2443

>NTDB_id=808811 P4829_RS00580 WP_063639030.1 105944..107323(+) (radA) [Bacillus atrophaeus strain MBLB1156]
MAKSKTKFICQSCGYESAKWMGKCPGCGAWNTMTEETVKKTPANRRAAFAHSTQTVKKPSPITSIETSEEPRIKTQLGEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLSDSTHSVLYISGEESVKQTKLRADRLGINNQTLHVLSETDMEYIS
SAIQEMNPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMRIAKTKGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGAAGSSIVASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLLMAVLEKRVGLLLQTQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDTPPNPADCIIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMIIPAANLDGWTKPKGIEVVGVANVAEALRTSLGGS

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=808811 P4829_RS00580 WP_063639030.1 105944..107323(+) (radA) [Bacillus atrophaeus strain MBLB1156]
ATGGCTAAATCAAAAACGAAATTTATCTGCCAATCCTGCGGCTATGAATCCGCAAAGTGGATGGGGAAATGTCCAGGCTG
CGGAGCTTGGAACACAATGACAGAAGAAACAGTAAAAAAAACACCGGCTAATCGCAGAGCAGCTTTTGCACACTCTACCC
AAACCGTAAAGAAACCTTCACCTATCACATCAATAGAAACATCTGAAGAACCCCGAATTAAAACACAGCTTGGAGAATTT
AACAGAGTGCTGGGCGGCGGTGTTGTGAAGGGCTCACTTGTCTTAATTGGAGGAGATCCCGGTATTGGTAAGTCAACCTT
GCTGCTTCAGGTATCCGCTCAGTTATCAGATTCCACGCATAGTGTGCTCTACATTTCAGGCGAGGAATCTGTGAAACAAA
CGAAACTCAGAGCAGATCGGCTCGGTATTAATAACCAAACGTTACATGTTTTATCTGAAACCGACATGGAGTATATTTCG
TCTGCTATACAAGAGATGAATCCAGCATTTGTGGTTGTTGACTCCATTCAGACGGTCTATCAAAGCGATATTACATCAGC
TCCGGGCAGTGTGTCGCAAGTCAGAGAGTGTACGGCAGAGCTGATGAGAATTGCCAAGACAAAGGGTATACCGATTTTTA
TCGTAGGACATGTAACAAAAGAAGGTTCAATTGCTGGCCCGAGACTGTTGGAACATATGGTTGATACTGTTCTTTACTTT
GAAGGAGAGCGGCATCATACGTTCCGCATCCTGCGGGCAGTAAAAAACCGTTTCGGCTCTACAAATGAAATGGGCATTTT
TGAAATGCGGGAAGAGGGTCTGACAGAGGTGCTGAATCCTTCTGAAATCTTTCTTGAGGAACGATCAGCCGGAGCGGCAG
GTTCCAGCATAGTAGCGTCTATGGAAGGCACCAGACCGATTCTCGTGGAAATACAGGCACTTATCTCACCGACCAGCTTT
GGAAATCCCCGGCGTATGGCCACGGGAATTGACCATAACAGAGTGTCACTGCTGATGGCTGTGTTAGAAAAAAGAGTCGG
GCTGCTGCTTCAAACTCAAGATGCGTATTTAAAGGTAGCAGGCGGCGTTAAGCTGGATGAACCGGCAATCGATCTCGCTG
TAGCTGTCAGTATTGCATCAAGCTTTAGGGATACACCGCCAAATCCTGCCGACTGCATTATAGGAGAAGTTGGTTTAACA
GGTGAGGTCCGCAGGGTATCCAGAATTGAACAGCGTGTGAAAGAAGCTGCTAAACTTGGATTTAAGCGTATGATCATACC
TGCGGCGAATTTAGATGGATGGACAAAACCAAAAGGGATTGAGGTTGTCGGAGTAGCAAATGTTGCAGAAGCGCTTCGTA
CTTCATTAGGAGGATCATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(366-430)

AAA domain (AAA_25)

(69-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

94.323

99.782

0.941
  radA Streptococcus mitis NCTC 12261

63.135

98.693

0.623
  radA Streptococcus pneumoniae Rx1

63.135

98.693

0.623
  radA Streptococcus pneumoniae D39

63.135

98.693

0.623
  radA Streptococcus pneumoniae R6

63.135

98.693

0.623
  radA Streptococcus pneumoniae TIGR4

63.135

98.693

0.623
  radA Streptococcus mitis SK321

62.914

98.693

0.621