Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   P3T49_RS13305 Genome accession   NZ_CP120723
Coordinates   2645842..2646465 (-) Length   207 a.a.
NCBI ID   WP_277713633.1    Uniprot ID   -
Organism   Bacillus atrophaeus strain NX-12     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2640842..2651465
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P3T49_RS13275 rpsT 2641136..2641402 (+) 267 WP_003325396.1 30S ribosomal protein S20 -
  P3T49_RS13280 holA 2641417..2642460 (-) 1044 WP_277713631.1 DNA polymerase III subunit delta -
  P3T49_RS13285 - 2642500..2642649 (-) 150 WP_010789254.1 hypothetical protein -
  P3T49_RS13290 - 2642690..2642824 (+) 135 WP_003229983.1 YqzM family protein -
  P3T49_RS13295 comEC 2642865..2645198 (-) 2334 WP_277713632.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  P3T49_RS13300 - 2645205..2645774 (-) 570 WP_003325392.1 ComE operon protein 2 -
  P3T49_RS13305 comEA 2645842..2646465 (-) 624 WP_277713633.1 helix-hairpin-helix domain-containing protein Machinery gene
  P3T49_RS13310 comER 2646549..2647370 (+) 822 WP_277713634.1 late competence protein ComER -
  P3T49_RS13315 - 2647440..2648180 (-) 741 WP_003325388.1 class I SAM-dependent methyltransferase -
  P3T49_RS13320 rsfS 2648177..2648533 (-) 357 WP_003325387.1 ribosome silencing factor -
  P3T49_RS13325 yqeK 2648550..2649110 (-) 561 WP_277713635.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  P3T49_RS13330 - 2649100..2649669 (-) 570 WP_003325385.1 nicotinate-nucleotide adenylyltransferase -
  P3T49_RS13335 yhbY 2649681..2649971 (-) 291 WP_003325384.1 ribosome assembly RNA-binding protein YhbY -
  P3T49_RS13340 aroE 2649965..2650807 (-) 843 WP_216413429.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 21973.73 Da        Isoelectric Point: 4.7220

>NTDB_id=808376 P3T49_RS13305 WP_277713633.1 2645842..2646465(-) (comEA) [Bacillus atrophaeus strain NX-12]
MNWFYQHKKIILTAGAAVISIVILLNFPGAKKQEPVQQITASPETAGTRVQQQESKGDADGEKIIIDIKGAVKHPGVYEL
KTGDRVSQAIEKAGGINSEADEKQVNLAELLQDGTVVYIPSEGEEPTQSAAANASVQNGAGKETLVNINTASLEELQAIS
GVGPSKAEAIIAYREENGKFQAVEDITNVSGIGEKSFEKIKSAITVK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=808376 P3T49_RS13305 WP_277713633.1 2645842..2646465(-) (comEA) [Bacillus atrophaeus strain NX-12]
ATGAATTGGTTTTACCAGCACAAAAAAATAATCTTAACGGCAGGTGCTGCTGTCATCTCCATCGTCATCCTGCTTAATTT
TCCCGGCGCAAAGAAGCAGGAGCCTGTTCAGCAAATCACAGCGTCGCCTGAAACGGCGGGCACACGAGTTCAGCAGCAAG
AATCAAAAGGTGATGCTGACGGAGAAAAAATTATCATTGATATTAAAGGCGCTGTCAAACATCCGGGTGTTTACGAATTG
AAAACGGGAGACAGAGTTTCACAGGCTATCGAAAAAGCGGGAGGCATTAATTCAGAGGCTGATGAAAAACAAGTAAATTT
AGCTGAACTCCTGCAGGACGGCACTGTCGTGTACATTCCGAGTGAGGGGGAAGAGCCAACACAAAGCGCTGCCGCAAATG
CTTCTGTGCAAAACGGAGCAGGAAAAGAGACTTTGGTTAATATCAATACAGCGTCATTAGAGGAACTGCAGGCTATTTCC
GGGGTCGGTCCGTCAAAAGCAGAAGCTATAATAGCTTATCGTGAAGAAAACGGAAAATTTCAAGCCGTTGAAGACATTAC
AAATGTCTCGGGAATCGGCGAAAAATCATTTGAGAAAATAAAATCAGCCATCACAGTTAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(143-205)

SLBB domain (SLBB)

(66-120)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

64.734

100

0.647
  comEA Staphylococcus aureus MW2

36.842

100

0.372