Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   P3T49_RS12865 Genome accession   NZ_CP120723
Coordinates   2564707..2565777 (-) Length   356 a.a.
NCBI ID   WP_063638548.1    Uniprot ID   -
Organism   Bacillus atrophaeus strain NX-12     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2559707..2570777
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P3T49_RS12820 tapA 2560242..2560943 (-) 702 WP_151290438.1 amyloid fiber anchoring/assembly protein TapA -
  P3T49_RS12825 - 2561205..2561528 (+) 324 WP_088117568.1 DUF3889 domain-containing protein -
  P3T49_RS12830 - 2561575..2561754 (-) 180 WP_003325435.1 YqzE family protein -
  P3T49_RS12835 comGG 2561824..2562198 (-) 375 WP_088117569.1 competence type IV pilus minor pilin ComGG Machinery gene
  P3T49_RS12840 comGF 2562199..2562705 (-) 507 WP_274379311.1 competence type IV pilus minor pilin ComGF -
  P3T49_RS12845 comGE 2562608..2562955 (-) 348 WP_088117570.1 competence type IV pilus minor pilin ComGE Machinery gene
  P3T49_RS12850 comGD 2562939..2563379 (-) 441 WP_088117571.1 competence type IV pilus minor pilin ComGD Machinery gene
  P3T49_RS12855 comGC 2563369..2563668 (-) 300 WP_003325429.1 competence type IV pilus major pilin ComGC Machinery gene
  P3T49_RS12860 comGB 2563683..2564720 (-) 1038 WP_277713604.1 competence type IV pilus assembly protein ComGB Machinery gene
  P3T49_RS12865 comGA 2564707..2565777 (-) 1071 WP_063638548.1 competence type IV pilus ATPase ComGA Machinery gene
  P3T49_RS12870 - 2566179..2567132 (-) 954 WP_088117575.1 magnesium transporter CorA family protein -
  P3T49_RS12875 - 2567254..2568602 (+) 1349 Protein_2514 hemolysin family protein -
  P3T49_RS12880 - 2568673..2569524 (-) 852 WP_277713607.1 STAS domain-containing protein -
  P3T49_RS12890 - 2569792..2570172 (-) 381 WP_277713608.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  P3T49_RS12895 - 2570410..2570655 (+) 246 WP_003325421.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40457.76 Da        Isoelectric Point: 9.4624

>NTDB_id=808372 P3T49_RS12865 WP_063638548.1 2564707..2565777(-) (comGA) [Bacillus atrophaeus strain NX-12]
MDTIEKTSRTLIEEAYSVKASDIHIVPREQDALIHFRIDHALLKKRTMKKEECVRLISHFKFLSAMDIGERRKPQSGSLS
LKLKKGTVYLRMSTLPTINDESLVIRVLPQHNIPAIERLSLFPKTGATLLSFLKHSHGLIIFTGPTGSGKSTTLYSLVQY
AKKHFNRNIITLEDPVETRDQDVLQVQVNEKAGITYSAGLKAILRHDPDMIVLGEIRDAETAKIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGVNMNEIEQTAIAIAAQRLVDLTCPFCENGCSSVYCRITRDVRRATIYELLYGKNLQQCIQEARGE
RANYQYRTLRQIIRKGIALGYLTTNNYDRWVYHEED

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=808372 P3T49_RS12865 WP_063638548.1 2564707..2565777(-) (comGA) [Bacillus atrophaeus strain NX-12]
TTGGATACGATAGAAAAAACTAGCAGGACATTAATTGAAGAGGCATATTCCGTGAAGGCCTCGGATATTCACATTGTACC
AAGGGAGCAAGATGCTTTGATTCATTTTCGGATTGACCATGCGTTACTGAAGAAGCGGACAATGAAAAAGGAAGAGTGCG
TTCGGCTCATTTCCCATTTTAAATTTCTGTCAGCCATGGATATAGGAGAAAGGCGGAAGCCGCAAAGTGGTTCACTTTCT
TTAAAGCTTAAAAAAGGGACAGTTTATCTCAGAATGTCGACACTTCCTACGATTAATGATGAAAGCCTCGTGATCAGAGT
TTTGCCTCAGCATAATATTCCTGCGATCGAAAGACTGTCCCTGTTCCCAAAGACAGGCGCCACGCTGCTATCCTTTTTAA
AGCACTCCCATGGCCTCATTATTTTTACCGGTCCGACTGGCTCAGGGAAATCTACCACCCTATATTCACTGGTTCAATAT
GCAAAAAAGCATTTCAACCGAAATATTATCACTCTCGAAGATCCTGTGGAAACAAGAGATCAGGATGTCTTGCAGGTACA
GGTGAATGAAAAAGCCGGCATTACGTACTCAGCGGGCCTGAAGGCGATTTTGCGCCATGATCCAGACATGATTGTTCTGG
GGGAAATCAGGGATGCTGAAACAGCAAAAATCGCCGTGCGGGCTGCTATGACAGGTCATTTGGTATTAACAAGCCTGCAC
ACAAGAGATGCCAAGGGCGCCATTTACAGACTGCTTGAATTCGGAGTAAATATGAATGAAATAGAGCAAACAGCGATTGC
GATAGCAGCCCAGCGATTAGTCGACTTAACCTGTCCATTTTGTGAAAACGGCTGTTCCTCTGTTTATTGCCGGATTACAC
GTGATGTAAGGCGAGCGACCATTTATGAGCTTCTTTACGGGAAAAATCTTCAGCAATGTATACAAGAAGCTAGAGGAGAA
CGGGCCAATTATCAGTACCGAACCTTACGGCAGATTATCAGAAAAGGAATCGCGCTTGGCTATCTAACGACGAATAATTA
CGACCGGTGGGTTTATCATGAAGAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

87.921

100

0.879