Detailed information NTG0808088

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: P4B24_RS02280
• Genome accession: NZ_CP120708
• Coordinates: 507908..509056 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain NY7723
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 502908..514056

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P4B24_RS02255 (P4B24_02255)	-	504059..504355 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
P4B24_RS02260 (P4B24_02260)	-	504352..504945 (-)	594	WP_003084544.1	YggT family protein	-
P4B24_RS02265 (P4B24_02265)	proC	504956..505777 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
P4B24_RS02270 (P4B24_02270)	-	505789..506481 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
P4B24_RS02275 (P4B24_02275)	pilT	506696..507730 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
P4B24_RS02280 (P4B24_02280)	pilU	507908..509056 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
P4B24_RS02285 (P4B24_02285)	czcD	509064..509963 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
P4B24_RS02290 (P4B24_02290)	-	510117..510521 (+)	405	WP_003084560.1	NINE protein	-
P4B24_RS02295 (P4B24_02295)	-	510764..512137 (+)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
P4B24_RS02300 (P4B24_02300)	-	512134..513318 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=808088 P4B24_RS02280 WP_003110016.1 507908..509056(+) (pilU) [Pseudomonas aeruginosa strain NY7723]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=808088 P4B24_RS02280 WP_003110016.1 507908..509056(+) (pilU) [Pseudomonas aeruginosa strain NY7723]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAATTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACTCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTACACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382