NTDB

Overview

Name	comGA	Type	Machinery gene
Locus tag	P5637_RS09465	Genome accession	NZ_CP120601
Coordinates	1835739..1836803 (-)	Length	354 a.a.
NCBI ID	WP_025811157.1	Uniprot ID	-
Organism	Bacillus paralicheniformis strain PRO109
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1830739..1841803

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P5637_RS09420 (P5637_09420)	tapA	1831234..1831962 (-)	729	WP_020452169.1	amyloid fiber anchoring/assembly protein TapA	-
P5637_RS09425 (P5637_09425)	-	1832239..1832559 (+)	321	WP_103749520.1	YqzG/YhdC family protein	-
P5637_RS09430 (P5637_09430)	-	1832589..1832771 (-)	183	WP_020452171.1	YqzE family protein	-
P5637_RS09435 (P5637_09435)	comGG	1832860..1833225 (-)	366	WP_026579313.1	competence type IV pilus minor pilin ComGG	-
P5637_RS09440 (P5637_09440)	comGF	1833237..1833725 (-)	489	WP_223254778.1	competence type IV pilus minor pilin ComGF	-
P5637_RS09445 (P5637_09445)	comGE	1833634..1833981 (-)	348	WP_020452174.1	competence type IV pilus minor pilin ComGE	-
P5637_RS09450 (P5637_09450)	comGD	1833965..1834402 (-)	438	WP_020452175.1	competence type IV pilus minor pilin ComGD	-
P5637_RS09455 (P5637_09455)	comGC	1834408..1834701 (-)	294	WP_026579311.1	competence type IV pilus major pilin ComGC	Machinery gene
P5637_RS09460 (P5637_09460)	comGB	1834715..1835749 (-)	1035	WP_025811158.1	competence type IV pilus assembly protein ComGB	Machinery gene
P5637_RS09465 (P5637_09465)	comGA	1835739..1836803 (-)	1065	WP_025811157.1	competence type IV pilus ATPase ComGA	Machinery gene
P5637_RS09470 (P5637_09470)	-	1836961..1837800 (-)	840	WP_020452179.1	STAS domain-containing protein	-
P5637_RS09480 (P5637_09480)	-	1838042..1838419 (-)	378	WP_003183482.1	Spx/MgsR family RNA polymerase-binding regulatory protein	-
P5637_RS09485 (P5637_09485)	-	1838630..1838875 (+)	246	WP_020452180.1	DUF2626 domain-containing protein	-
P5637_RS09490 (P5637_09490)	-	1838911..1839549 (-)	639	WP_145645267.1	MBL fold metallo-hydrolase	-
P5637_RS09495 (P5637_09495)	-	1839705..1839878 (+)	174	WP_003183486.1	DUF2759 domain-containing protein	-
P5637_RS09500 (P5637_09500)	-	1839939..1840253 (-)	315	WP_020452182.1	MTH1187 family thiamine-binding protein	-
P5637_RS09505 (P5637_09505)	-	1840269..1841378 (-)	1110	WP_020452183.1	hypothetical protein	-

Sequence

Protein

Download Length: 354 a.a. Molecular weight: 39797.28 Da Isoelectric Point: 9.1063

                                >NTDB_id=806647 P5637_RS09465 WP_025811157.1 1835739..1836803(-) (comGA) [Bacillus paralicheniformis strain PRO109]

MYTIESLSGRLIDEAYRMKASDIHIVPGEKEAVVRFRIDDELFQKDRLTRNECSRLISHFKFLSSMDIGERRLPQSGALT

LYINRQPVHLRMSTLPTIHDESLVIRLLPKMSSKPLTKLSLFPSATFKLLSFLKHSHGLILFTGPTGSGKTTTLYSLIEY

AKRHFKRNIITLEDPVESRSENILQVQVNEKAGMTYSAGLKAVLRHDPDMIILGEIRDAETAQIAVRAALTGHLVLSSMH

AKNAKGAIYRLLEFGVDMTEIEQTLVAISAQRLVGLVCPFCGDSCSLYCRLSRPVRRASVFELLYGKSLNLCIEEAKGRC

GDIKTETLKTLIQKGIALGYLPSNTYERWIGHED

Nucleotide

Download Length: 1065 bp

                                >NTDB_id=806647 P5637_RS09465 WP_025811157.1 1835739..1836803(-) (comGA) [Bacillus paralicheniformis strain PRO109]

TTGTACACGATTGAATCATTAAGCGGAAGATTAATTGACGAGGCATATAGAATGAAGGCGTCAGACATTCATATTGTTCC

CGGTGAAAAGGAGGCGGTGGTCCGCTTCAGGATTGATGATGAACTTTTTCAAAAAGACAGATTAACGAGAAATGAGTGCT

CAAGGCTCATTTCTCACTTTAAATTCCTTTCTTCGATGGATATTGGGGAAAGACGGCTGCCGCAAAGCGGAGCTTTAACT

CTTTATATCAACCGTCAGCCCGTTCATTTAAGAATGTCCACTCTGCCCACCATCCATGATGAAAGCTTGGTCATCAGGCT

TCTTCCCAAGATGAGCAGCAAGCCGCTCACAAAGCTCTCATTGTTTCCGAGTGCAACATTCAAGCTGCTGTCTTTTCTGA

AGCATTCCCACGGATTAATTCTGTTCACCGGTCCGACAGGCTCGGGGAAAACGACAACACTCTATTCTTTAATTGAATAC

GCTAAGCGGCATTTTAAGCGAAATATCATTACCTTGGAAGACCCTGTTGAATCAAGAAGCGAAAACATTTTGCAAGTGCA

AGTAAACGAAAAAGCTGGCATGACTTATTCTGCGGGGTTGAAGGCGGTGCTTCGCCACGATCCGGACATGATCATCCTAG

GGGAAATTCGCGATGCCGAAACAGCCCAAATCGCAGTCAGGGCAGCTTTGACAGGACACCTTGTTTTATCGAGCATGCAT

GCGAAAAATGCCAAAGGCGCAATATACAGGCTGCTCGAGTTCGGCGTTGATATGACAGAAATTGAACAAACACTGGTCGC

AATCAGCGCCCAGCGCCTCGTCGGTCTCGTCTGCCCGTTTTGCGGCGACAGCTGCTCTTTATATTGCAGATTGTCGAGGC

CTGTGAGAAGAGCAAGCGTCTTCGAGCTTTTGTACGGAAAAAGCCTCAATCTCTGCATCGAAGAAGCAAAAGGCAGGTGC

GGTGACATAAAAACAGAAACTTTGAAAACGCTGATCCAGAAGGGAATAGCACTCGGATATCTGCCGTCGAATACTTATGA

ACGCTGGATAGGCCATGAAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-274)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGA	Bacillus subtilis subsp. subtilis str. 168	67.797	100	0.678
pilB	Vibrio cholerae strain A1552	38.671	93.503	0.362

Detailed information NTG0806647