Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   P3U05_RS06000 Genome accession   NZ_CP120179
Coordinates   1280270..1281244 (+) Length   324 a.a.
NCBI ID   WP_014613820.1    Uniprot ID   -
Organism   Staphylococcus pseudintermedius strain Dog018     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1275270..1286244
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P3U05_RS05965 (P3U05_05965) rpmG 1275669..1275818 (+) 150 WP_014613813.1 50S ribosomal protein L33 -
  P3U05_RS05970 (P3U05_05970) - 1275970..1276509 (+) 540 WP_014613814.1 5-formyltetrahydrofolate cyclo-ligase -
  P3U05_RS05975 (P3U05_05975) - 1276528..1277967 (+) 1440 WP_014613815.1 rhomboid family intramembrane serine protease -
  P3U05_RS05980 (P3U05_05980) - 1277969..1278172 (+) 204 WP_014613816.1 YqgQ family protein -
  P3U05_RS05985 (P3U05_05985) - 1278169..1279155 (+) 987 WP_014613817.1 ROK family glucokinase -
  P3U05_RS05990 (P3U05_05990) - 1279156..1279467 (+) 312 WP_014613818.1 MTH1187 family thiamine-binding protein -
  P3U05_RS05995 (P3U05_05995) - 1279480..1280109 (+) 630 WP_014613819.1 MBL fold metallo-hydrolase -
  P3U05_RS06000 (P3U05_06000) comGA 1280270..1281244 (+) 975 WP_014613820.1 competence type IV pilus ATPase ComGA Machinery gene
  P3U05_RS06005 (P3U05_06005) comGB 1281213..1282280 (+) 1068 WP_014613821.1 competence type IV pilus assembly protein ComGB -
  P3U05_RS06010 (P3U05_06010) comGC 1282293..1282616 (+) 324 WP_014613822.1 competence type IV pilus major pilin ComGC Machinery gene
  P3U05_RS06015 (P3U05_06015) comGD 1282594..1283034 (+) 441 WP_014613823.1 competence type IV pilus minor pilin ComGD -
  P3U05_RS06020 (P3U05_06020) - 1283139..1283339 (+) 201 WP_020219623.1 hypothetical protein -
  P3U05_RS06025 (P3U05_06025) - 1283245..1283730 (+) 486 WP_080556831.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  P3U05_RS06030 (P3U05_06030) - 1283894..1284421 (+) 528 WP_014613826.1 shikimate kinase -
  P3U05_RS06035 (P3U05_06035) gcvT 1284579..1285670 (+) 1092 WP_014613827.1 glycine cleavage system aminomethyltransferase GcvT -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36846.58 Da        Isoelectric Point: 7.5584

>NTDB_id=804218 P3U05_RS06000 WP_014613820.1 1280270..1281244(+) (comGA) [Staphylococcus pseudintermedius strain Dog018]
MQLLLDHVIQFALQNEASDIHFIPSQSQVEVKLRVKDQLVLYDTLNLETYQKLLTLLKYQAGLDISTRHKAQSGRYIYEH
KALYYLRVSTLPLNLGTESCVIRITPQYFQNAKAHSVLDIKSNMEKKQGLILFSGPTGAGKSTLMYQMVVYAKQKLNLNI
ITIEDPVEQLVNGITQISVNEKADITYGSSLKAILRCDPDVILIGEIRDSHIARDVIQASLSGHLVLSTIHANDCQGVLL
RLIEMGLSHQDLSQSINLIINQRLITTKNDQRQLVYETMDQQEIHYFLNNNFQLPTAFTSLSHKLQLLNKEGVIDEKILR
KYTI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=804218 P3U05_RS06000 WP_014613820.1 1280270..1281244(+) (comGA) [Staphylococcus pseudintermedius strain Dog018]
ATGCAACTACTATTAGATCATGTAATACAATTTGCACTTCAAAATGAGGCATCAGACATTCATTTTATCCCATCACAATC
ACAAGTCGAAGTGAAACTCAGAGTGAAAGATCAACTTGTATTATATGACACATTAAATCTAGAAACGTATCAAAAATTGT
TAACTTTGTTGAAATACCAAGCTGGATTAGATATTTCGACACGCCACAAAGCGCAAAGTGGGCGCTATATTTATGAACAT
AAAGCGTTATATTATTTAAGAGTGTCCACGTTACCTTTAAACTTAGGTACTGAAAGTTGTGTTATTCGTATTACACCCCA
ATATTTTCAAAATGCGAAAGCTCATAGTGTATTAGACATCAAATCTAATATGGAGAAAAAGCAAGGTTTAATTCTGTTTA
GCGGGCCTACAGGTGCAGGAAAGAGCACGTTAATGTATCAAATGGTCGTCTATGCTAAACAAAAGTTAAACCTGAATATT
ATCACAATAGAAGACCCGGTCGAGCAGCTTGTGAACGGCATTACGCAAATTTCCGTTAACGAAAAAGCTGATATTACGTA
TGGATCGTCGTTAAAAGCAATTCTTCGTTGTGATCCAGATGTCATCTTAATTGGTGAGATTCGTGACAGTCATATTGCAA
GGGACGTTATACAAGCGAGTTTAAGTGGGCATTTAGTATTGTCGACGATACACGCCAATGATTGTCAAGGGGTGCTCCTT
AGACTGATTGAAATGGGACTCTCTCACCAAGATTTATCTCAATCTATCAATCTCATCATCAATCAAAGACTCATTACAAC
AAAAAACGACCAGCGACAACTCGTCTATGAAACGATGGATCAACAGGAGATTCATTACTTTTTGAATAATAACTTCCAAC
TACCTACTGCTTTCACAAGCCTTTCTCATAAGCTTCAACTTCTGAATAAAGAGGGGGTTATAGATGAAAAGATTTTACGA
AAGTATACGATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

58.514

99.691

0.583
  comGA Staphylococcus aureus N315

58.514

99.691

0.583