Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   P3U54_RS06810 Genome accession   NZ_CP120155
Coordinates   1365085..1365981 (+) Length   298 a.a.
NCBI ID   WP_239706323.1    Uniprot ID   -
Organism   Mammaliicoccus lentus strain Dog026     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1360085..1370981
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P3U54_RS06770 (P3U54_06770) - 1360142..1360696 (+) 555 WP_064204534.1 5-formyltetrahydrofolate cyclo-ligase -
  P3U54_RS06775 (P3U54_06775) - 1360697..1362145 (+) 1449 WP_323711066.1 rhomboid family intramembrane serine protease -
  P3U54_RS06780 (P3U54_06780) - 1362148..1362339 (+) 192 WP_017000229.1 YqgQ family protein -
  P3U54_RS06785 (P3U54_06785) - 1362336..1363307 (+) 972 WP_017000230.1 ROK family glucokinase -
  P3U54_RS06790 (P3U54_06790) - 1363307..1363621 (+) 315 WP_017000231.1 MTH1187 family thiamine-binding protein -
  P3U54_RS06795 (P3U54_06795) - 1363650..1363889 (-) 240 WP_323711067.1 DUF2759 domain-containing protein -
  P3U54_RS06800 (P3U54_06800) - 1363987..1364619 (+) 633 WP_323711068.1 MBL fold metallo-hydrolase -
  P3U54_RS06805 (P3U54_06805) - 1364684..1364932 (-) 249 WP_017000234.1 DUF2626 family protein -
  P3U54_RS06810 (P3U54_06810) comGA 1365085..1365981 (+) 897 WP_239706323.1 competence type IV pilus ATPase ComGA Machinery gene
  P3U54_RS06815 (P3U54_06815) comGB 1365978..1367018 (+) 1041 WP_323711069.1 competence type IV pilus assembly protein ComGB -
  P3U54_RS06820 (P3U54_06820) comGC 1367032..1367349 (+) 318 WP_064211051.1 competence type IV pilus major pilin ComGC Machinery gene
  P3U54_RS06825 (P3U54_06825) comGD 1367349..1367777 (+) 429 WP_323711070.1 competence type IV pilus minor pilin ComGD -
  P3U54_RS06830 (P3U54_06830) comGE 1367764..1368036 (+) 273 WP_228082628.1 competence type IV pilus minor pilin ComGE -
  P3U54_RS06835 (P3U54_06835) comGF 1368050..1368445 (+) 396 WP_237687866.1 competence type IV pilus minor pilin ComGF -
  P3U54_RS06840 (P3U54_06840) - 1368681..1369199 (+) 519 WP_064205405.1 shikimate kinase -
  P3U54_RS06845 (P3U54_06845) gcvT 1369362..1370450 (+) 1089 WP_323711071.1 glycine cleavage system aminomethyltransferase GcvT -

Sequence

Protein


Download         Length: 298 a.a.        Molecular weight: 34452.68 Da        Isoelectric Point: 6.3035

>NTDB_id=803882 P3U54_RS06810 WP_239706323.1 1365085..1365981(+) (comGA) [Mammaliicoccus lentus strain Dog026]
METLFKTIIKDAIILKATDIHFIPVKEDRVKVQLRISGKNTIYDCDINNALYNRLLIYMKFIAHLDVSERNKAQSGQYIF
DDIYKYYLRISTLPLSLGIESCSIRLTPKYFEEYIDHNIDDNYKMIKEEMFNSEGLFLFTGPTGSGKSTIMYQFLYDLQQ
IKDKHIITIEDPVEQVLDGVVQVSVNEKANINYHNSFKAILRCDPDVVMIGEIRDEETAKQVIQASLSGHLILSTMHAKD
NLGAIYRLLELGISKEQIKQGIACIANQRLLNDQLGNRYRQMTYIFSHEINLYLENKS

Nucleotide


Download         Length: 897 bp        

>NTDB_id=803882 P3U54_RS06810 WP_239706323.1 1365085..1365981(+) (comGA) [Mammaliicoccus lentus strain Dog026]
ATGGAAACTTTATTTAAGACAATTATCAAAGATGCTATTATTTTGAAAGCAACTGACATTCATTTTATTCCAGTGAAAGA
GGATCGGGTTAAAGTTCAGTTGAGGATAAGTGGTAAGAACACGATTTATGATTGTGATATTAATAACGCTTTATATAACC
GTTTATTAATTTATATGAAGTTTATTGCTCATCTAGATGTAAGTGAAAGGAATAAAGCTCAAAGTGGTCAATATATATTT
GATGATATATATAAATATTATTTAAGAATATCCACTTTACCTTTAAGTCTAGGAATTGAGTCTTGCTCAATTAGGTTAAC
GCCTAAATATTTCGAAGAATATATTGACCATAATATTGATGACAATTATAAAATGATTAAAGAAGAAATGTTTAATTCCG
AAGGCTTATTTTTGTTCACAGGACCAACCGGTTCGGGTAAATCGACGATTATGTACCAATTTTTATATGACTTACAACAA
ATTAAAGATAAACATATTATTACGATTGAAGACCCAGTTGAACAAGTTTTAGATGGTGTAGTACAAGTTTCTGTAAATGA
AAAAGCAAACATAAATTATCATAATTCTTTTAAAGCCATTTTAAGATGTGATCCAGATGTTGTCATGATAGGAGAAATTA
GAGATGAAGAAACAGCCAAGCAAGTTATACAAGCAAGTTTAAGTGGTCATTTAATATTATCTACAATGCATGCGAAAGAT
AATTTAGGGGCTATTTATAGACTTTTAGAACTTGGAATATCTAAAGAGCAAATAAAACAAGGTATCGCATGTATAGCCAA
TCAAAGACTTTTAAATGACCAATTAGGAAATAGATATAGACAAATGACTTATATATTTAGTCATGAAATTAATCTATATT
TAGAAAATAAATCATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

50.993

100

0.517
  comGA Staphylococcus aureus N315

50.993

100

0.517