Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrA   Type   Machinery gene
Locus tag   P3U54_RS00325 Genome accession   NZ_CP120155
Coordinates   67586..68935 (-) Length   449 a.a.
NCBI ID   WP_323710789.1    Uniprot ID   -
Organism   Mammaliicoccus lentus strain Dog026     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 47768..67566 67586..68935 flank 20


Gene organization within MGE regions


Location: 47768..68935
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P3U54_RS00225 (P3U54_00225) mecA 47768..49777 (-) 2010 WP_063852655.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  P3U54_RS00230 (P3U54_00230) mecR1 49874..51631 (+) 1758 WP_000952923.1 beta-lactam sensor/signal transducer MecR1 -
  P3U54_RS00235 (P3U54_00235) mecI 51631..52002 (+) 372 WP_000369214.1 mecA-type methicillin resistance repressor MecI -
  P3U54_RS00240 (P3U54_00240) psm-mec 52087..52155 (-) 69 WP_014532405.1 phenol-soluble modulin PSM-mec -
  P3U54_RS00245 (P3U54_00245) - 52475..53623 (+) 1149 WP_000427807.1 ROK family transcriptional regulator -
  P3U54_RS00250 (P3U54_00250) cstB 53737..55071 (-) 1335 Protein_49 persulfide dioxygenase-sulfurtransferase CstB -
  P3U54_RS00255 (P3U54_00255) - 55105..56169 (-) 1065 WP_000438836.1 DsrE/DsrF/DrsH-like family protein -
  P3U54_RS00260 (P3U54_00260) cstR 56305..56565 (+) 261 WP_000220507.1 persulfide-sensing transcriptional repressor CstR -
  P3U54_RS00265 (P3U54_00265) - 56565..57203 (+) 639 Protein_52 sulfite exporter TauE/SafE family protein -
  P3U54_RS00270 (P3U54_00270) - 57477..58094 (-) 618 WP_000562107.1 CadD family cadmium resistance transporter -
  P3U54_RS00275 (P3U54_00275) - 58175..60589 (-) 2415 WP_000378485.1 cadmium-translocating P-type ATPase CadA -
  P3U54_RS00280 (P3U54_00280) - 60582..60947 (-) 366 WP_000159127.1 ArsR/SmtB family transcription factor -
  P3U54_RS00285 (P3U54_00285) - 61185..61562 (-) 378 WP_001041908.1 DUF6262 family protein -
  P3U54_RS00290 (P3U54_00290) - 61569..63461 (-) 1893 WP_002485298.1 site-specific integrase -
  P3U54_RS00295 (P3U54_00295) - 63656..63979 (-) 324 WP_000088091.1 JAB domain-containing protein -
  P3U54_RS00300 (P3U54_00300) - 63972..64178 (-) 207 WP_001077283.1 hypothetical protein -
  P3U54_RS00305 (P3U54_00305) - 64180..64701 (-) 522 WP_000836008.1 DUF1643 domain-containing protein -
  P3U54_RS00310 (P3U54_00310) - 64721..65032 (-) 312 WP_201101506.1 DUF960 family protein -
  P3U54_RS00315 (P3U54_00315) - 65117..65467 (-) 351 WP_194376028.1 SAUGI family uracil-DNA glycosylase inhibitor -
  P3U54_RS00320 (P3U54_00320) ccrB 65938..67566 (-) 1629 WP_323710788.1 cassette chromosome recombinase CcrB Machinery gene
  P3U54_RS00325 (P3U54_00325) ccrA 67586..68935 (-) 1350 WP_323710789.1 cassette chromosome recombinase CcrA Machinery gene

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 52260.44 Da        Isoelectric Point: 10.2047

>NTDB_id=803869 P3U54_RS00325 WP_323710789.1 67586..68935(-) (ccrA) [Mammaliicoccus lentus strain Dog026]
MKRAIGYLRQSTTKQQSLPAQKQAIELLAPKHNIQHILYISDKQSGRTDKRNGYLQITKLIQQEQYDVLCCYRLNRLHRN
LKNALELMKLCQKHHVHILSVCDGYFDMDKAFDRLKLNIFMSMAELESDNISEQVNNGIKEKARQGKLITTHAPFGYHYK
HGTFTINKNESLTVIAIFNYYLQGNGYKKISQFLEEDIKLLNRKPYQVRNIIMNPNYCGRVSNKYGTFDNMFPPIISKAM
YKKAQAIRVNKQVQRTPSANLLKQKLKCPCCRSTLTNMTISKKQHTLRYYVCPRNMNESRFVCDFKGINAQQLEDQVLAI
CKDFFQNKQIYPKINNKIRQQLKQHQHVETQNSVTQAQLIDKLAQGLIDAESFRKQTQSMNQKRKTIQSISDIQLQTSLQ
NVIQQSFTLNMLYPHIDEIHITKNKALVGIYFKNEPLNIVNQTSQSSIA

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=803869 P3U54_RS00325 WP_323710789.1 67586..68935(-) (ccrA) [Mammaliicoccus lentus strain Dog026]
ATGAAACGAGCCATTGGTTATTTACGCCAAAGTACAACGAAACAACAATCACTCCCAGCTCAAAAGCAAGCGATAGAATT
ATTAGCTCCAAAGCACAATATTCAACATATCCTATACATTAGTGATAAACAATCAGGACGCACAGATAAGCGAAATGGAT
ATTTACAAATTACTAAACTTATTCAACAAGAACAATACGACGTGTTATGTTGTTACAGACTTAATCGATTACATCGTAAT
TTGAAAAATGCTTTGGAACTTATGAAGTTATGCCAAAAGCATCATGTTCACATCTTAAGTGTATGTGATGGCTATTTCGA
TATGGATAAAGCTTTTGATCGTCTAAAACTCAATATCTTTATGAGTATGGCAGAACTTGAGTCAGATAACATTAGTGAAC
AAGTAAACAACGGGATAAAAGAAAAAGCACGTCAGGGTAAATTGATAACTACACACGCTCCCTTTGGCTACCACTACAAA
CATGGTACATTCACAATAAATAAAAATGAGTCACTAACGGTAATTGCCATATTCAATTACTACCTTCAAGGTAATGGCTA
TAAAAAAATTTCACAGTTCTTAGAAGAAGATATCAAACTACTCAACCGGAAACCTTATCAAGTACGTAATATCATCATGA
ACCCTAATTATTGCGGACGTGTGAGTAACAAATATGGCACTTTTGATAATATGTTCCCACCTATTATCAGTAAAGCTATG
TATAAAAAGGCTCAAGCAATACGAGTAAATAAACAAGTTCAGCGCACGCCTTCTGCTAATTTACTTAAACAAAAATTAAA
ATGTCCTTGTTGTCGCTCCACTCTTACAAATATGACAATTAGTAAAAAGCAACATACATTACGTTATTATGTCTGTCCTC
GAAATATGAATGAATCTCGTTTTGTCTGTGACTTCAAGGGTATAAATGCGCAACAACTAGAAGATCAAGTCTTAGCCATA
TGTAAAGACTTCTTTCAAAACAAACAAATCTATCCAAAAATAAATAACAAAATCAGACAACAACTCAAGCAACACCAACA
TGTAGAAACACAAAATAGTGTTACGCAAGCCCAACTGATAGATAAATTGGCTCAAGGTCTGATTGATGCTGAATCATTCA
GAAAACAGACGCAGTCGATGAATCAAAAGCGTAAAACCATACAATCAATAAGTGATATTCAGCTACAAACTTCACTACAA
AATGTGATACAGCAAAGTTTCACGTTAAACATGCTGTATCCCCATATTGATGAAATTCATATTACTAAAAACAAAGCCCT
TGTTGGGATATATTTCAAAAATGAACCATTGAACATTGTGAACCAAACCTCGCAATCATCGATTGCCTAA

Domains

Predicted by InterproScan.

Resolvase, N terminal domain (Resolvase)

(4-147)

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(261-319)

Recombinase (Recombinase)

(169-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrA Staphylococcus aureus COL

72.606

100

0.726
  ccrA Staphylococcus aureus N315

69.042

100

0.69