Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   RFN65_RS14905 Genome accession   NZ_CP133703
Coordinates   2885345..2887174 (-) Length   609 a.a.
NCBI ID   WP_003245178.1    Uniprot ID   -
Organism   Bacillus subtilis strain CP35     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 2880345..2892174
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFN65_RS14870 (RFN65_14870) yjbM 2880798..2881433 (-) 636 WP_003245294.1 GTP diphosphokinase -
  RFN65_RS14875 (RFN65_14875) - 2881462..2881830 (-) 369 WP_003232922.1 hypothetical protein -
  RFN65_RS14880 (RFN65_14880) yjbK 2881955..2882527 (+) 573 WP_015252351.1 CYTH domain-containing protein -
  RFN65_RS14885 (RFN65_14885) cwlQ 2882542..2883276 (+) 735 WP_309529710.1 bifunctional muramidase/murein lytic transglycosylase -
  RFN65_RS14890 (RFN65_14890) bhbI 2883531..2883929 (+) 399 WP_063335043.1 thiol management oxidoreductase -
  RFN65_RS14895 (RFN65_14895) spxH 2883926..2884825 (+) 900 WP_309529711.1 protease adaptor protein SpxH -
  RFN65_RS14900 (RFN65_14900) - 2885140..2885307 (+) 168 WP_003244944.1 hypothetical protein -
  RFN65_RS14905 (RFN65_14905) pepF 2885345..2887174 (-) 1830 WP_003245178.1 oligoendopeptidase F Regulator
  RFN65_RS14910 (RFN65_14910) coiA 2887404..2888555 (-) 1152 WP_100506184.1 competence protein CoiA Machinery gene
  RFN65_RS14915 (RFN65_14915) - 2888599..2888722 (+) 124 Protein_2947 hypothetical protein -
  RFN65_RS14920 (RFN65_14920) mecA 2888717..2889373 (-) 657 WP_003232942.1 adaptor protein MecA Regulator
  RFN65_RS14925 (RFN65_14925) - 2889340..2889480 (-) 141 WP_119122854.1 hypothetical protein -
  RFN65_RS14930 (RFN65_14930) yjbE 2889650..2890306 (+) 657 WP_003232944.1 TerC family protein -
  RFN65_RS14935 (RFN65_14935) spx 2890349..2890744 (-) 396 WP_014476435.1 transcriptional regulator Spx -
  RFN65_RS14940 (RFN65_14940) yjbC 2890925..2891503 (-) 579 WP_181218831.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 70085.95 Da        Isoelectric Point: 5.1562

>NTDB_id=800166 RFN65_RS14905 WP_003245178.1 2885345..2887174(-) (pepF) [Bacillus subtilis strain CP35]
MAEEKKANQLPDRSEVKAEDTWRLEDIFPSDEAWNKEFQAVKELIPNLSKYKGKLADSADHLYEALTYQDKVMERLGRLY
TYAHMRSDQDTGNSFYQGLNDKAGNLYTQAASATAYLVPEILSIEEDKLQQFILEKEELKLYSHAIEEITKERPHVLSEK
EEALLAEASEVLGSSSNTFSVLNNADITFPSIKDEDGNEKQITHGNFINFLESENREVRKNAFDAVYKTYGQYKNTMATT
LSGTVKKDNFYARVKKYKSAREAALSNNSIPEEVYDNLVKTINKHLPLLHRYIALRKKVLELDEVHIYDLYTPLVKDAGM
KVTYEEAKDYMLKGLAPLGEEYASILKEGLENRWVDVYENKGKRNGAYSSGAYGTNPYILMNWHNNVNNLFTLVHEFGHS
VHSYYTRKHQPYPYGNYSIFVAEVASTTNEALLGEYLLNNLEDEKQRLYILNHMLEGFRGTVFRQTMFAEFEHLIHTKAQ
EGEPLTPELLTNVYYDLNKKYFGDGMVIDKEIGLEWSRIPHFYYNYYVYQYATGYSAAQALSSQILKEGKPAVDRYIDFL
KAGSSQYPIDVLKKAGVDMTSPEPIEAACKMFEEKLDEMEELLMKVKQS

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=800166 RFN65_RS14905 WP_003245178.1 2885345..2887174(-) (pepF) [Bacillus subtilis strain CP35]
ATGGCTGAGGAAAAAAAAGCAAACCAACTGCCTGACAGAAGTGAGGTAAAGGCAGAAGACACATGGAGACTTGAGGATAT
TTTTCCTAGTGATGAGGCCTGGAATAAAGAATTTCAAGCTGTAAAAGAATTAATTCCGAATTTATCTAAGTATAAAGGAA
AGCTGGCAGATTCAGCTGATCATTTATACGAGGCTCTTACGTATCAAGATAAAGTGATGGAGCGGCTGGGTAGGCTGTAC
ACATATGCGCATATGCGCTCTGACCAGGATACTGGGAACTCCTTTTACCAGGGGCTGAATGACAAGGCGGGAAACCTGTA
TACACAGGCCGCAAGCGCGACAGCTTATTTGGTTCCGGAGATTTTATCCATAGAAGAAGACAAACTGCAGCAGTTCATTC
TTGAAAAAGAAGAATTGAAGCTGTACTCTCATGCGATTGAGGAGATTACAAAGGAACGTCCGCATGTGCTGAGCGAGAAG
GAAGAGGCGCTGCTTGCTGAAGCCTCCGAGGTACTGGGGTCATCTTCAAATACATTCAGCGTGTTAAATAACGCAGATAT
AACGTTTCCGTCCATTAAAGACGAAGATGGGAATGAAAAACAGATCACTCACGGCAACTTTATTAATTTCTTGGAAAGTG
AAAACCGTGAAGTCCGCAAAAATGCGTTTGACGCAGTGTATAAAACGTACGGACAATATAAAAACACAATGGCCACGACG
CTTAGCGGCACTGTGAAAAAGGACAACTTCTACGCGAGAGTGAAAAAGTACAAGTCAGCGCGTGAGGCTGCGCTTTCTAA
CAACAGTATTCCTGAGGAAGTATACGATAACCTTGTTAAGACGATTAATAAGCATTTGCCGCTCCTGCACCGCTATATTG
CGCTGAGAAAGAAAGTGCTTGAGCTTGATGAAGTGCATATCTATGACCTGTATACACCGCTTGTGAAAGATGCGGGGATG
AAGGTGACATACGAGGAAGCCAAAGATTACATGCTGAAAGGCCTCGCACCATTAGGGGAAGAATATGCCTCAATCCTAAA
AGAAGGACTGGAAAACCGCTGGGTAGACGTTTACGAGAATAAAGGCAAACGCAATGGGGCTTATTCATCAGGAGCTTACG
GCACGAATCCGTATATTTTGATGAACTGGCATAATAATGTCAATAATCTCTTTACGCTCGTGCACGAGTTTGGACATTCC
GTACACAGCTACTATACGAGAAAGCACCAGCCTTATCCATACGGCAATTACAGTATCTTTGTCGCGGAAGTTGCCTCTAC
GACAAATGAAGCGCTCCTTGGCGAGTATTTGCTGAACAATTTAGAGGATGAAAAACAGCGCTTATATATTCTCAACCATA
TGCTTGAGGGCTTCAGGGGAACGGTCTTCAGACAAACGATGTTCGCTGAATTTGAACATCTGATCCATACAAAGGCGCAA
GAAGGCGAGCCGCTTACACCTGAGCTTCTGACAAATGTCTATTACGACCTGAATAAAAAGTATTTTGGAGACGGCATGGT
GATTGATAAGGAAATCGGCCTTGAATGGTCGCGTATTCCGCACTTCTATTACAATTACTATGTGTATCAGTATGCGACAG
GGTACAGCGCTGCCCAAGCATTAAGCAGCCAGATTTTGAAGGAAGGAAAGCCGGCGGTTGACCGTTATATTGACTTCCTG
AAAGCGGGAAGCTCACAATACCCGATTGATGTCCTGAAAAAAGCGGGCGTTGATATGACGTCTCCAGAGCCAATCGAAGC
CGCGTGCAAAATGTTTGAAGAAAAACTTGATGAAATGGAAGAGTTGCTGATGAAAGTCAAGCAGTCTTAA

Domains

Predicted by InterProScan.

Peptidase family M3 (Peptidase_M3)

(211-590)

Oligopeptidase F (Peptidase_M3_N)

(120-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

48.822

97.537

0.476