Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   PX690_RS03435 Genome accession   NZ_CP119675
Coordinates   611204..612274 (-) Length   356 a.a.
NCBI ID   WP_276123467.1    Uniprot ID   -
Organism   Bacillus velezensis strain 160     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 606204..617274
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PX690_RS03385 (PX690_03385) - 606264..606848 (-) 585 WP_012117977.1 signal peptidase I -
  PX690_RS03390 (PX690_03390) tapA 606820..607491 (-) 672 WP_276123466.1 amyloid fiber anchoring/assembly protein TapA -
  PX690_RS03395 (PX690_03395) - 607750..608079 (+) 330 WP_003153097.1 DUF3889 domain-containing protein -
  PX690_RS03400 (PX690_03400) - 608119..608298 (-) 180 WP_003153093.1 YqzE family protein -
  PX690_RS03405 (PX690_03405) comGG 608355..608732 (-) 378 WP_014305410.1 competence type IV pilus minor pilin ComGG Machinery gene
  PX690_RS03410 (PX690_03410) comGF 608733..609233 (-) 501 WP_226565836.1 competence type IV pilus minor pilin ComGF -
  PX690_RS03415 (PX690_03415) comGE 609142..609456 (-) 315 WP_015388003.1 competence type IV pilus minor pilin ComGE Machinery gene
  PX690_RS03420 (PX690_03420) comGD 609440..609877 (-) 438 WP_224462754.1 competence type IV pilus minor pilin ComGD Machinery gene
  PX690_RS03425 (PX690_03425) comGC 609867..610133 (-) 267 WP_042635730.1 competence type IV pilus major pilin ComGC Machinery gene
  PX690_RS03430 (PX690_03430) comGB 610180..611217 (-) 1038 WP_003153086.1 competence type IV pilus assembly protein ComGB Machinery gene
  PX690_RS03435 (PX690_03435) comGA 611204..612274 (-) 1071 WP_276123467.1 competence type IV pilus ATPase ComGA Machinery gene
  PX690_RS03440 (PX690_03440) - 612466..613416 (-) 951 WP_003153082.1 magnesium transporter CorA family protein -
  PX690_RS03445 (PX690_03445) - 613562..614863 (+) 1302 WP_014305416.1 hemolysin family protein -
  PX690_RS03450 (PX690_03450) - 614901..615731 (-) 831 WP_003153080.1 STAS domain-containing protein -
  PX690_RS03460 (PX690_03460) - 615937..616317 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  PX690_RS03465 (PX690_03465) - 616554..616799 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40513.79 Da        Isoelectric Point: 9.5781

>NTDB_id=800163 PX690_RS03435 WP_276123467.1 611204..612274(-) (comGA) [Bacillus velezensis strain 160]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQSFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=800163 PX690_RS03435 WP_276123467.1 611204..612274(-) (comGA) [Bacillus velezensis strain 160]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGTTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCACACGGCCTGCTTCTGTTTACCGGCCCGACCGGCTCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGAGATGCCGAAACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTTGGGGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACCCGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAAAGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACAACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

81.742

100

0.817
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379