Detailed information    

insolico Bioinformatically predicted

Overview

Name   hexB   Type   Machinery gene
Locus tag   RFN65_RS11670 Genome accession   NZ_CP133703
Coordinates   2293596..2295479 (-) Length   627 a.a.
NCBI ID   WP_100506572.1    Uniprot ID   -
Organism   Bacillus subtilis strain CP35     
Function   DNA mismatch repair (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2288596..2300479
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFN65_RS11630 (RFN65_11630) - 2288959..2289297 (-) 339 WP_251217917.1 hypothetical protein -
  RFN65_RS11635 (RFN65_11635) - 2289530..2290072 (-) 543 WP_100506578.1 tyrosine-type recombinase/integrase -
  RFN65_RS11640 (RFN65_11640) - 2290072..2290452 (-) 381 WP_285840562.1 ArpU family phage packaging/lysis transcriptional regulator -
  RFN65_RS11645 (RFN65_11645) - 2290754..2291503 (-) 750 WP_157799635.1 hypothetical protein -
  RFN65_RS11650 (RFN65_11650) - 2291775..2292137 (+) 363 WP_100506576.1 Ig-like domain-containing protein -
  RFN65_RS11655 (RFN65_11655) - 2292181..2292468 (-) 288 WP_100506575.1 YrzE family protein -
  RFN65_RS11660 (RFN65_11660) - 2292652..2292977 (-) 326 Protein_2299 VWA domain-containing protein -
  RFN65_RS11665 (RFN65_11665) - 2293020..2293217 (-) 198 WP_100506573.1 XtrA/YqaO family protein -
  RFN65_RS11670 (RFN65_11670) hexB 2293596..2295479 (-) 1884 WP_100506572.1 DNA mismatch repair endonuclease MutL Machinery gene
  RFN65_RS11675 (RFN65_11675) hexA 2295495..2298071 (-) 2577 WP_033883742.1 DNA mismatch repair protein MutS Machinery gene
  RFN65_RS11680 (RFN65_11680) cotE 2298204..2298749 (-) 546 WP_003231833.1 outer spore coat protein CotE -
  RFN65_RS11685 (RFN65_11685) ymcA 2299011..2299442 (-) 432 WP_003231834.1 regulatory iron-sulfur-containing complex subunit RicA Regulator

Sequence

Protein


Download         Length: 627 a.a.        Molecular weight: 70470.33 Da        Isoelectric Point: 5.6008

>NTDB_id=800142 RFN65_RS11670 WP_100506572.1 2293596..2295479(-) (hexB) [Bacillus subtilis strain CP35]
MAKVIQLSDELSNKIAAGEVVERPASVVKELVENAIDADSTVIEIDIEEAGLASIRVLDNGEGMENEDCKRAFRRHATSK
IKDENDLFRVRTLGFRGEALPSIASVSHLEITTSTGEGAGTKLVLQGGNIISESRSSSRKGTEIVVSNLFFNTPARLKYM
KTVHTELGNITDVVNRIALAHPEVSIRLRHHGKNLLQTNGNGDVRHVLAAIYGTAVAKKMLPLHVSSLDFEVKGYIALPE
ITRASRNYMSSVVNGRYIKNFPLVKAVHEGYHTLLPIGRHPITFIEITMDPILVDVNVHPSKLEVRLSKETELHDLIRDG
IKDVFKQQQLIPSAQLPKKSAPVIKNEQQFITFDEKPAEKKVPEKSTASSYSPMKLSSVVKEPVDAEEELPPLQFDAPPI
VDQEQTLEVSDVSAEEPETFEQECHEEQQQPASERVPIMYPIGQMHGTYILAQNENGLYIIDQHAAQERIKYEYFREKVG
EVEPEVQDMIVPLTFHYSTNEALIIEQHKQELESVGVFLESFGSNSYIVRCHPAWFPKGEEAELIEEIIQQVLDSKNIDI
KKLREEAAIMMSCKGSIKANRHLRNDEIKALLDDLRSTSDPFTCPHGRPIIIHHSTYEMEKMFKRVM

Nucleotide


Download         Length: 1884 bp        

>NTDB_id=800142 RFN65_RS11670 WP_100506572.1 2293596..2295479(-) (hexB) [Bacillus subtilis strain CP35]
GTGGCAAAAGTCATCCAGCTGTCAGATGAGCTTTCAAATAAAATAGCGGCGGGCGAGGTTGTGGAACGGCCCGCCTCAGT
CGTCAAAGAATTGGTGGAAAATGCGATCGACGCTGACAGCACAGTCATTGAAATCGATATTGAGGAAGCAGGCCTTGCAT
CCATTCGGGTTTTGGATAACGGCGAAGGAATGGAGAATGAAGATTGCAAGCGTGCTTTCCGCCGCCACGCAACGAGTAAA
ATAAAAGATGAAAATGATTTATTCAGGGTGAGAACGCTCGGTTTCAGGGGAGAAGCCCTGCCGAGTATCGCGTCAGTCTC
TCATCTGGAGATTACAACAAGCACTGGTGAAGGAGCTGGGACGAAGCTCGTCCTCCAAGGAGGAAACATCATTTCCGAAT
CGCGTTCCTCAAGCAGGAAGGGAACTGAAATTGTCGTTTCCAACCTGTTTTTTAACACACCGGCCCGTTTGAAATATATG
AAAACCGTTCATACAGAGCTTGGAAACATTACAGATGTGGTGAACCGTATTGCTCTGGCGCATCCAGAGGTATCGATCCG
CCTGCGCCATCATGGGAAAAACCTGCTTCAAACGAACGGAAACGGAGATGTGCGCCATGTACTGGCAGCGATTTACGGCA
CGGCTGTCGCCAAAAAAATGCTCCCGCTGCATGTGAGCTCCTTGGATTTTGAAGTAAAGGGATATATCGCCCTTCCAGAG
ATTACACGGGCGTCGAGAAACTATATGTCGTCTGTGGTCAATGGCCGTTACATTAAAAATTTCCCGCTCGTCAAAGCGGT
TCATGAAGGCTATCATACGCTTCTGCCGATCGGACGCCATCCGATTACTTTTATAGAAATTACGATGGACCCGATTTTAG
TCGATGTCAACGTGCATCCATCGAAGCTTGAGGTCCGGCTCAGCAAGGAAACAGAGCTTCATGACTTAATACGTGACGGA
ATTAAAGATGTGTTTAAACAGCAGCAGCTGATTCCGAGTGCCCAGCTCCCGAAAAAATCGGCACCTGTAATCAAAAATGA
GCAGCAGTTCATAACCTTTGATGAAAAACCTGCGGAAAAAAAAGTTCCGGAAAAATCGACTGCGTCATCTTATTCACCAA
TGAAGCTCAGTTCAGTCGTGAAAGAGCCGGTTGATGCGGAGGAAGAATTGCCTCCTCTCCAATTTGATGCTCCTCCTATC
GTTGATCAGGAACAAACCCTTGAGGTGTCCGATGTTTCAGCAGAAGAGCCTGAAACATTTGAGCAGGAATGCCATGAGGA
ACAGCAGCAGCCAGCGTCTGAGCGGGTTCCGATTATGTACCCGATCGGCCAGATGCACGGGACTTATATATTGGCACAAA
ACGAAAACGGCCTGTATATTATCGATCAGCACGCCGCCCAAGAACGTATTAAATATGAGTACTTTCGTGAAAAGGTGGGG
GAGGTTGAGCCTGAGGTGCAGGATATGATTGTACCGCTGACGTTCCACTACTCCACAAATGAGGCTTTGATTATTGAACA
GCACAAACAAGAGCTCGAAAGCGTCGGTGTCTTTTTAGAGTCATTCGGCTCTAACAGTTACATCGTCCGTTGCCACCCGG
CCTGGTTTCCAAAGGGAGAAGAAGCAGAGCTTATAGAAGAAATCATTCAGCAGGTGCTCGACTCCAAAAATATAGATATT
AAAAAACTCCGCGAGGAAGCGGCGATTATGATGAGCTGCAAAGGCTCCATCAAAGCAAATCGCCACCTCAGAAACGACGA
AATCAAAGCGCTTCTGGACGACCTCCGAAGCACTTCAGACCCATTTACATGCCCGCACGGCCGCCCGATCATCATACACC
ATTCCACTTATGAGATGGAAAAGATGTTTAAACGCGTGATGTAG

Domains

Predicted by InterProScan.

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c_3)

(25-124)

MutL C terminal dimerisation domain (MutL_C)

(442-583)

DNA mismatch repair protein, C-terminal domain (DNA_mis_repair)

(209-324)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  hexB Streptococcus pneumoniae R6

45.497

100

0.467