Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   P1J77_RS01270 Genome accession   NZ_CP119597
Coordinates   263616..264941 (+) Length   441 a.a.
NCBI ID   WP_159330814.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain 1039     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 258616..269941
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P1J77_RS01245 (P1J77_01245) - 259386..260027 (+) 642 WP_002983671.1 response regulator transcription factor -
  P1J77_RS01250 (P1J77_01250) - 260064..261464 (+) 1401 WP_011285657.1 Cof-type HAD-IIB family hydrolase -
  P1J77_RS01255 (P1J77_01255) - 261464..261841 (+) 378 WP_002991882.1 S1 RNA-binding domain-containing protein -
  P1J77_RS01260 (P1J77_01260) cysK 261859..262800 (-) 942 WP_010922535.1 cysteine synthase A -
  P1J77_RS01265 (P1J77_01265) - 262928..263560 (-) 633 WP_009880468.1 YigZ family protein -
  P1J77_RS01270 (P1J77_01270) comFA/cflA 263616..264941 (+) 1326 WP_159330814.1 DEAD/DEAH box helicase Machinery gene
  P1J77_RS01275 (P1J77_01275) - 264913..265578 (+) 666 WP_002983685.1 ComF family protein -
  P1J77_RS01280 (P1J77_01280) raiA 265658..266206 (+) 549 WP_002988974.1 ribosome-associated translation inhibitor RaiA -

Sequence

Protein


Download         Length: 441 a.a.        Molecular weight: 49767.32 Da        Isoelectric Point: 10.0009

>NTDB_id=799939 P1J77_RS01270 WP_159330814.1 263616..264941(+) (comFA/cflA) [Streptococcus pyogenes strain 1039]
MESIENSYGRLLLESQLPDSAKQLAQPLKSVVILRGKMICQRCHYQLDEEARLPSGAYYCRFCLVFGRNQSDKLLYAIPP
MNFPKGNYLVWGGQLTAYQEMISQQLLINMQNQKTTLVHAVTGAGKTEMIYAAIEAVINTGGWVCIASPRVDVCVEVATR
LSQAFSCSICLMHAESLPYQRAPIIVATTHQLLKFHKAFDLLIIDEVDAFPFVNNIQLHYAASQALKEGGAKILLTATST
RTLERKVNKGEVVKLTLARRFHNRPLVIPKFIRSFNLFKMIHRQKLPLKILKYLKKQRKTGYPLLIFLPTIIMAESVTAI
LKELLPAEQIACVSSQSQNRKEDITAFRQGKKTILITTSILERGVTFPQIDVFVLGSHHRVYSSQSLVQIAGRVGRSIDR
PDGTLYFFHEGISKAMLLARKEIKEMNYKGYSHDLSTMSTN

Nucleotide


Download         Length: 1326 bp        

>NTDB_id=799939 P1J77_RS01270 WP_159330814.1 263616..264941(+) (comFA/cflA) [Streptococcus pyogenes strain 1039]
ATGGAGTCTATTGAAAATAGTTATGGACGGTTGTTGCTAGAAAGCCAGTTACCAGATTCAGCAAAGCAGCTTGCTCAACC
TTTAAAAAGTGTAGTAATATTGAGAGGAAAAATGATTTGTCAACGTTGTCACTATCAGCTTGATGAAGAAGCACGATTGC
CAAGTGGAGCCTATTATTGCCGATTTTGTCTTGTCTTTGGACGAAATCAATCAGATAAATTGCTCTATGCTATCCCACCT
ATGAATTTTCCAAAAGGTAATTATCTGGTTTGGGGCGGTCAACTAACTGCTTATCAAGAGATGATTTCCCAACAATTATT
AATCAATATGCAAAATCAAAAAACAACATTAGTTCATGCTGTAACTGGTGCAGGAAAAACAGAAATGATTTATGCTGCGA
TTGAGGCGGTAATTAACACAGGTGGCTGGGTTTGCATTGCTAGCCCAAGAGTAGATGTTTGTGTAGAGGTGGCTACTCGT
TTATCCCAAGCCTTCTCCTGCAGCATCTGTTTAATGCACGCAGAGTCTCTTCCTTATCAAAGAGCTCCTATTATAGTAGC
CACAACGCATCAGCTGCTAAAATTTCACAAGGCTTTTGATCTCTTAATTATTGATGAAGTAGACGCATTCCCTTTTGTAA
ATAATATTCAACTTCATTATGCTGCAAGTCAGGCTTTAAAAGAAGGTGGCGCAAAGATTTTATTAACTGCAACTTCAACT
AGAACTTTAGAAAGAAAGGTAAACAAGGGAGAAGTAGTCAAGCTGACATTGGCGAGACGCTTTCATAATAGGCCATTAGT
TATTCCAAAATTTATAAGAAGTTTTAATCTATTTAAAATGATACATCGGCAAAAACTTCCTCTTAAAATTTTAAAATACT
TAAAAAAACAAAGAAAAACAGGCTATCCCCTTTTAATTTTTCTACCCACTATTATAATGGCAGAATCTGTTACAGCAATC
TTAAAAGAGTTATTGCCCGCCGAACAAATAGCTTGTGTTTCTAGTCAATCACAAAACAGGAAAGAAGATATCACAGCTTT
TCGTCAAGGGAAAAAAACGATTTTAATTACAACATCAATTTTAGAAAGGGGTGTGACATTTCCCCAAATTGATGTTTTTG
TTCTTGGGTCTCATCATCGTGTCTATAGTTCCCAAAGTCTTGTTCAAATTGCTGGACGAGTTGGAAGATCTATAGACAGA
CCAGACGGAACGTTATATTTCTTTCATGAAGGTATCAGTAAAGCAATGCTACTAGCAAGAAAAGAGATTAAAGAAATGAA
TTATAAAGGGTATTCGCATGATCTGTCTACTATGTCAACAAATTAG

Domains

Predicted by InterproScan.

DEAD/DEAH box helicase (DEAD)

(98-244)

Helicase conserved C-terminal domain (Helicase_C)

(288-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

53.699

95.011

0.51
  comFA/cflA Streptococcus pneumoniae D39

53.699

95.011

0.51
  comFA/cflA Streptococcus pneumoniae R6

53.699

95.011

0.51
  comFA/cflA Streptococcus pneumoniae TIGR4

53.699

95.011

0.51
  comFA/cflA Streptococcus mitis NCTC 12261

52.37

95.692

0.501
  comFA/cflA Streptococcus mitis SK321

51.422

95.692

0.492
  comFA Lactococcus lactis subsp. cremoris KW2

47.98

89.796

0.431
  comFA Latilactobacillus sakei subsp. sakei 23K

38.851

98.639

0.383