Detailed information    

insolico Bioinformatically predicted

Overview

Name   uvrC   Type   Machinery gene
Locus tag   RB265_RS07270 Genome accession   NZ_CP133128
Coordinates   1339385..1341217 (-) Length   610 a.a.
NCBI ID   WP_001283421.1    Uniprot ID   P0A8G1
Organism   Escherichia coli O157:H7 strain PNUSAE146743     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1334385..1346217
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RB265_RS07225 (RB265_07225) - 1334601..1335785 (-) 1185 WP_000005444.1 phage tail sheath family protein -
  RB265_RS07230 (RB265_07230) - 1335943..1336209 (+) 267 Protein_1430 phage late control D family protein -
  RB265_RS07235 (RB265_07235) - 1336217..1337350 (-) 1134 WP_032161728.1 IS30-like element IS30 family transposase -
  RB265_RS07240 (RB265_07240) - 1337292..1337486 (-) 195 WP_115801851.1 hypothetical protein -
  RB265_RS07245 (RB265_07245) - 1337507..1337788 (-) 282 WP_001303543.1 hypothetical protein -
  RB265_RS07265 (RB265_07265) pgsA 1338780..1339328 (-) 549 WP_001160187.1 CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase -
  RB265_RS07270 (RB265_07270) uvrC 1339385..1341217 (-) 1833 WP_001283421.1 excinuclease ABC subunit UvrC Machinery gene
  RB265_RS07275 (RB265_07275) letA 1341214..1341870 (-) 657 WP_000611328.1 UvrY/SirA/GacA family response regulator transcription factor Regulator
  RB265_RS07280 (RB265_07280) - 1342012..1342128 (+) 117 WP_001302039.1 hypothetical protein -
  RB265_RS07285 (RB265_07285) yecU 1342166..1342342 (+) 177 WP_001302050.1 protein YecU -
  RB265_RS07290 (RB265_07290) yecF 1342329..1342553 (+) 225 WP_000106479.1 DUF2594 family protein YecF -
  RB265_RS07295 (RB265_07295) sdiA 1342621..1343343 (-) 723 WP_001154288.1 transcriptional regulator SdiA -
  RB265_RS07300 (RB265_07300) tcyN 1343573..1344325 (-) 753 WP_001273005.1 L-cystine ABC transporter ATP-binding protein TcyN -
  RB265_RS07305 (RB265_07305) tcyL 1344322..1344990 (-) 669 WP_001158226.1 cystine ABC transporter permease -
  RB265_RS07310 (RB265_07310) dcyD 1345005..1345991 (-) 987 WP_001128225.1 D-cysteine desulfhydrase -

Sequence

Protein


Download         Length: 610 a.a.        Molecular weight: 68188.03 Da        Isoelectric Point: 9.3685

>NTDB_id=798512 RB265_RS07270 WP_001283421.1 1339385..1341217(-) (uvrC) [Escherichia coli O157:H7 strain PNUSAE146743]
MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLSSYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLL
EHNYIKLYQPRYNVLLRDDKSYPFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETASQNLEFEEAARIRDQIQAVRRVT
EKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKVLGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLD
FNLSDKTLLADSLSELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEVKRMECFDI
SHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRYGKAIDDSKIPDVILIDGGKGQLAQAKNVFA
ELDVSWDKNHPLLLGVAKGADRKAGLETLFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLET
IEGVGPKRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH

Nucleotide


Download         Length: 1833 bp        

>NTDB_id=798512 RB265_RS07270 WP_001283421.1 1339385..1341217(-) (uvrC) [Escherichia coli O157:H7 strain PNUSAE146743]
GTGAGTGATCAGTTTGACGCAAAAGCGTTTTTAAAAACCGTAACCAGCCAGCCTGGCGTTTATCGCATGTACGATGCTGG
TGGTACGGTTATCTATGTCGGCAAAGCGAAAGACCTGAAAAAACGGCTTTCCAGCTATTTCCGTAGCAACCTCGCTTCGC
GCAAAACCGAAGCGCTGGTCGCCCAGATCCAGCAAATTGATGTAACGGTTACTCATACAGAAACCGAAGCGCTGTTGCTG
GAACACAACTACATCAAACTCTATCAGCCGCGTTACAACGTTTTGCTACGCGATGATAAATCATATCCTTTTATCTTCCT
GAGTGGCGATACCCACCCGCGTCTGGCGATGCATCGTGGTGCAAAGCATGCCAAAGGTGAATATTTCGGCCCGTTCCCGA
ATGGCTATGCCGTACGTGAAACACTGGCGCTACTGCAAAAGATTTTCCCCATTCGCCAGTGCGAAAATAGTGTTTATCGC
AATCGCTCGCGTCCGTGTCTGCAATATCAGATAGGGCGCTGTCTGGGACCGTGCGTTGAAGGACTGGTGAGTGAAGAAGA
ATACGCTCAGCAGGTCGAGTATGTGCGCCTGTTTTTGTCTGGCAAAGATGATCAGGTGCTTACGCAACTCATTAGCCGTA
TGGAAACTGCCAGTCAGAATCTGGAGTTTGAAGAAGCGGCACGTATTCGCGACCAAATTCAGGCGGTGCGACGCGTCACC
GAAAAACAATTCGTTTCCAATACCGGCGACGACCTCGACGTTATTGGTGTGGCGTTCGATGCGGGTATGGCTTGTGTCCA
CGTATTGTTCATTCGTCAGGGCAAAGTGCTCGGCAGCCGCAGCTATTTCCCGAAAGTGCCTGGCGGTACGGAACTGAGCG
AGGTGGTAGAAACCTTCGTAGGCCAGTTCTATTTACAAGGCAGCCAGATGCGCACCTTACCGGGTGAGATCCTGCTCGAT
TTTAATCTTAGCGATAAAACGCTGCTCGCCGATTCCCTTTCAGAACTGGCGGGACGCAAGATTAATGTTCAAACCAAACC
TCGTGGCGATAGGGCGCGTTACCTGAAACTCGCGCGCACCAATGCGGCGACGGCCTTAACCAGCAAACTTTCGCAGCAAT
CTACCGTTCACCAGCGGCTTACAGCACTTGCCAGCGTGTTGAAATTGCCGGAAGTGAAGCGGATGGAGTGCTTTGACATC
AGCCATACCATGGGTGAACAAACCGTCGCTTCCTGTGTGGTGTTTGATGCTAACGGCCCGCTGCGTGCGGAGTATCGGCG
CTATAACATTACTGGCATCACGCCGGGCGATGATTATGCGGCGATGAATCAGGTGCTGCGTCGGCGTTATGGTAAAGCCA
TCGACGACAGTAAGATCCCGGATGTGATACTTATCGACGGCGGCAAAGGCCAGCTTGCGCAGGCGAAAAATGTCTTCGCC
GAACTGGATGTCTCATGGGATAAAAATCATCCGCTGCTACTTGGTGTTGCCAAAGGAGCAGATCGTAAGGCTGGGCTGGA
AACGCTGTTCTTTGAGCCGGAAGGTGAGGGATTCAGTTTGCCGCCAGATTCTCCCGCGCTGCATGTTATCCAGCATATTC
GCGATGAATCACACGATCACGCGATTGGCGGGCACCGTAAAAAACGGGCGAAGGTCAAAAATACCAGTTCCCTGGAAACC
ATTGAAGGCGTCGGGCCAAAACGCCGGCAAATGTTGTTGAAATATATGGGCGGTTTGCAAGGTTTACGTAACGCCAGCGT
CGAGGAAATTGCAAAAGTGCCGGGTATTTCGCAAGGTCTGGCAGAAAAGATCTTCTGGTCGTTGAAACATTGA

Domains

Predicted by InterProScan.

UvrB/uvrC motif (UVR)

(205-237)

Helix-hairpin-helix domain (HHH_5)

(558-609)

UvrC RNAse H endonuclease domain (UvrC_RNaseH_dom)

(385-542)

GIY-YIG catalytic domain (GIY-YIG)

(18-93)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P0A8G1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  uvrC Streptococcus pneumoniae TIGR4

37.891

99.508

0.377
  uvrC Streptococcus pneumoniae R6

37.562

99.508

0.374
  uvrC Streptococcus pneumoniae D39

37.562

99.508

0.374