Detailed information    

insolico Bioinformatically predicted

Overview

Name   recN   Type   Machinery gene
Locus tag   L6477_RS20445 Genome accession   NZ_AP022886
Coordinates   3962866..3964605 (-) Length   579 a.a.
NCBI ID   WP_000947754.1    Uniprot ID   A0A437SJ22
Organism   Bacillus cereus strain MRY14-0074     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3957866..3969605
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L6477_RS20425 (BCM0074_4046) - 3957877..3958239 (-) 363 WP_236797208.1 GIY-YIG nuclease family protein -
  L6477_RS20430 (BCM0074_4047) - 3959239..3960213 (-) 975 WP_236797209.1 tyrosine-type recombinase/integrase -
  L6477_RS20435 (BCM0074_4048) spo0A 3960367..3961161 (-) 795 WP_003161566.1 sporulation transcription factor Spo0A Regulator
  L6477_RS20440 (BCM0074_4049) spoIVB 3961449..3962747 (-) 1299 WP_002054681.1 SpoIVB peptidase -
  L6477_RS20445 (BCM0074_4050) recN 3962866..3964605 (-) 1740 WP_000947754.1 DNA repair protein RecN Machinery gene
  L6477_RS20450 (BCM0074_4051) argR 3964872..3965321 (-) 450 WP_001032581.1 arginine repressor ArgR -
  L6477_RS20455 (BCM0074_4052) - 3965464..3966303 (-) 840 WP_001274742.1 TlyA family rRNA (cytidine-2'-O)-methyltransferase -
  L6477_RS20460 (BCM0074_4053) dxs 3966307..3968199 (-) 1893 WP_000366447.1 1-deoxy-D-xylulose-5-phosphate synthase -
  L6477_RS20465 (BCM0074_4054) ispA 3968501..3969391 (-) 891 WP_000150355.1 (2E,6E)-farnesyl diphosphate synthase -

Sequence

Protein


Download         Length: 579 a.a.        Molecular weight: 65815.89 Da        Isoelectric Point: 4.8574

>NTDB_id=79762 L6477_RS20445 WP_000947754.1 3962866..3964605(-) (recN) [Bacillus cereus strain MRY14-0074]
MLSELSIRNFAIIEALNISFQKGLTVLSGETGAGKSIIIDAISLLVGGRGSAEFVRYGTEKAEIEGLFYVEDDKHPCIEK
AEELDIEIEDGMIILKRDIAANGKSVCRVNGKLVTLSVLKEIGKTLVDIHGQHETQDLMNEERHLFMLDHFDGERIVKQL
DIYQNVYADYEKLKKQLKSLSENEQQMAHRLDLIQFQHEEIRKADLKMDEENNLTEERLQISNFEKIYKALGDAYRSLSA
DGQGLDNVRSAMGQMESITHLDEVYQENHDSIANSYYLLEEVAYQLREKLDMMEYDPNRLDEIETRLNEIRMLKRKYGNN
VEEILAYADKIEQEIFTIENKDVHIETTKKQLKELESVILKEATLLSNMRHELAEHLTNAIHQELKELYMEKTKFEVRII
KREGNAEEPLVEGVPVRLTADGYDHVEFYISTNPGEPLKPLSKVASGGELSRIILALKSIFSKHQGVASVIFDEVDTGVS
GRVAQAIAEKIYRVSVNSQVLCITHLPQVASMADSHLFIRKQVANDRTITSVTVLTMEDKVTEIARMISGVEITDLTTEH
AKELLTQAHHFKQTAEAIQ

Nucleotide


Download         Length: 1740 bp        

>NTDB_id=79762 L6477_RS20445 WP_000947754.1 3962866..3964605(-) (recN) [Bacillus cereus strain MRY14-0074]
TTGTTATCGGAATTATCGATTAGAAACTTTGCTATTATTGAGGCATTAAATATTTCTTTTCAAAAAGGATTAACGGTTTT
AAGTGGTGAAACAGGGGCCGGAAAATCGATTATTATTGATGCGATTAGTTTACTTGTAGGTGGCCGCGGTTCGGCAGAAT
TTGTTCGATACGGAACAGAAAAAGCTGAGATAGAGGGGCTATTTTATGTAGAAGATGATAAGCATCCATGTATTGAAAAG
GCAGAAGAATTGGATATAGAAATAGAAGACGGCATGATTATTTTGAAGCGTGATATCGCTGCAAACGGAAAAAGCGTATG
TCGTGTAAATGGAAAACTAGTTACCCTTAGTGTATTAAAAGAAATTGGAAAAACACTTGTTGATATTCATGGGCAGCACG
AAACGCAAGATTTAATGAATGAAGAACGTCATCTATTTATGCTCGATCATTTTGATGGGGAGCGTATCGTAAAACAATTG
GATATATACCAAAACGTATACGCTGACTATGAGAAGTTAAAAAAACAATTGAAATCGTTAAGTGAAAATGAACAACAAAT
GGCGCATCGCTTAGATTTAATTCAATTCCAGCATGAAGAAATCCGTAAGGCGGATTTAAAGATGGATGAAGAAAATAACT
TAACTGAAGAACGATTGCAAATTTCTAATTTCGAAAAGATTTATAAAGCATTAGGTGACGCATATCGCTCGTTAAGTGCT
GATGGACAAGGATTAGATAATGTAAGAAGTGCAATGGGACAAATGGAGAGTATTACACATTTAGACGAAGTGTACCAAGA
AAATCATGATTCAATTGCAAATAGCTATTACCTATTAGAAGAAGTTGCATATCAACTAAGAGAAAAACTCGATATGATGG
AATATGATCCAAATCGTTTAGACGAAATTGAAACGCGTTTAAATGAAATTCGTATGCTAAAGAGAAAATATGGAAATAAT
GTAGAAGAGATATTAGCGTATGCTGATAAAATTGAACAAGAGATTTTTACGATTGAAAATAAAGACGTTCATATTGAAAC
GACGAAGAAACAGTTGAAGGAATTAGAAAGTGTTATTCTGAAAGAAGCAACATTGTTAAGTAATATGCGTCATGAACTTG
CAGAGCATCTTACAAATGCCATTCATCAGGAATTAAAAGAATTGTATATGGAAAAAACAAAATTTGAAGTGAGAATCATA
AAGCGAGAAGGAAATGCGGAAGAGCCTCTCGTGGAGGGAGTACCGGTAAGGCTTACAGCGGACGGTTATGATCATGTGGA
ATTTTATATTTCAACCAATCCAGGTGAGCCGCTAAAACCACTTTCAAAGGTCGCTTCTGGTGGAGAGTTGTCCCGTATTA
TTTTAGCTTTAAAAAGTATTTTTTCTAAGCATCAAGGTGTTGCATCTGTTATTTTTGATGAAGTGGATACTGGTGTAAGT
GGCCGGGTCGCACAGGCTATTGCGGAAAAAATTTATCGAGTATCAGTAAACTCACAAGTACTTTGTATTACGCACTTACC
TCAAGTAGCTTCAATGGCGGATTCGCATTTATTTATCCGAAAACAAGTAGCGAATGATCGAACAATTACATCCGTTACCG
TTTTAACTATGGAGGATAAAGTAACAGAAATAGCTCGAATGATTTCTGGTGTGGAAATTACAGATTTAACGACAGAACAT
GCGAAAGAATTACTTACGCAAGCGCATCATTTTAAACAGACAGCAGAGGCTATCCAGTAA

Domains

Predicted by InterproScan.

RecF/RecN/SMC N terminal domain (SMC_N)

(1-521)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A437SJ22

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recN Bacillus subtilis subsp. subtilis str. 168

59.931

100

0.599

Multiple sequence alignment