Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PYU99_RS07990 Genome accession   NZ_CP118993
Coordinates   1711915..1713054 (-) Length   379 a.a.
NCBI ID   WP_171276387.1    Uniprot ID   -
Organism   Aeromonas media strain K521     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1706915..1718054
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PYU99_RS07965 (PYU99_07965) - 1707040..1707369 (-) 330 WP_270823812.1 translation initiation factor -
  PYU99_RS07970 (PYU99_07970) - 1707490..1707717 (+) 228 WP_171276390.1 FeoA family protein -
  PYU99_RS07975 (PYU99_07975) feoB 1707714..1709984 (+) 2271 WP_270823813.1 Fe(2+) transporter permease subunit FeoB -
  PYU99_RS07980 (PYU99_07980) - 1710028..1710270 (+) 243 WP_171276388.1 FeoC-like transcriptional regulator -
  PYU99_RS07985 (PYU99_07985) glgC 1710373..1711644 (-) 1272 WP_270823814.1 glucose-1-phosphate adenylyltransferase -
  PYU99_RS07990 (PYU99_07990) pilU 1711915..1713054 (-) 1140 WP_171276387.1 type IVa pilus ATPase TapW Machinery gene
  PYU99_RS07995 (PYU99_07995) - 1713109..1713465 (-) 357 WP_005328056.1 DMT family protein -
  PYU99_RS08000 (PYU99_08000) - 1713578..1714309 (+) 732 WP_270823815.1 substrate-binding periplasmic protein -
  PYU99_RS08005 (PYU99_08005) btuC 1714395..1715402 (+) 1008 WP_270823816.1 vitamin B12 ABC transporter permease BtuC -
  PYU99_RS08010 (PYU99_08010) - 1715389..1716174 (+) 786 WP_270823817.1 ATP-binding cassette domain-containing protein -
  PYU99_RS08015 (PYU99_08015) - 1716356..1717420 (-) 1065 WP_270823818.1 3-deoxy-7-phosphoheptulonate synthase -
  PYU99_RS08020 (PYU99_08020) - 1717562..1717942 (+) 381 WP_005328065.1 diacylglycerol kinase -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42370.39 Da        Isoelectric Point: 6.4288

>NTDB_id=797200 PYU99_RS07990 WP_171276387.1 1711915..1713054(-) (pilU) [Aeromonas media strain K521]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGALRPLNEVPLEPGEVARIAADLMDSEQKQQFEKELEMNLAISLPQIGR
FRVNIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTEGGRRAAVEVMIGTHTIRDLIKRGEFGGLKDIMEKSRALGMVTFDSTL
FDLVVEGVIDEEEAVKNADSQNNLRLKIKLWKEKGQIGNSAEASGWSLEPSKDENNDIF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=797200 PYU99_RS07990 WP_171276387.1 1711915..1713054(-) (pilU) [Aeromonas media strain K521]
ATGGAATTGCGAGACATGCTACAGATCCTGGCCAAGCAGGATGGCTCCGACCTCTATCTCTCCACCGGGGCCCCCCCCTG
CGCCAAGTTCAATGGCGCCCTGCGCCCCTTGAACGAGGTGCCGCTCGAGCCGGGCGAAGTGGCCAGGATCGCCGCCGATC
TCATGGACTCGGAGCAGAAGCAGCAGTTCGAGAAAGAGCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGATAGGCCGT
TTTCGGGTCAACATCTTCAAACAGCGCAACGAAGTCTCTCTGGTGGCGCGCAACATCAAGACCGAGATCCCCCGTTTCGA
GGATCTCAAACTCCCCCCGGTGCTGCTCGACACCATCATGGAGAAGCGCGGCCTGGTGCTCTTCGTCGGCGGTACCGGCT
CGGGCAAGTCCACCTCGCTGGCGGCCCTCATCGACCATCGCAACCGCAACAGCGGTGGCCACATCATCACCATCGAGGAT
CCGGTGGAGTTCGTCCATCGCCACCGCAAGAGCATCATCAACCAGCGAGAGGTGGGGGTGGACACCCGCAGCTTCCATGC
GGCCCTGAAAAACACCCTGCGCCAGGCACCGGACGTCATCCTCATCGGTGAGATCCGTGACCGGGAGACCATGGAGCATG
CGCTGGCCTTCTCGGAGACCGGGCATCTGGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCCGAGGAGCGCAGGCCCCAGCTGCTCAACGATCTTGGCAACAACCTCAAGGCCTTCGTCTCCCAGCG
GCTGGTGAAGACCACCGAGGGCGGCCGCCGGGCGGCGGTGGAGGTCATGATCGGCACCCACACCATTCGCGATCTCATCA
AGCGCGGCGAGTTTGGCGGCCTCAAGGATATCATGGAGAAATCCAGGGCCCTTGGCATGGTCACCTTCGACAGCACCCTG
TTCGATCTGGTGGTGGAGGGGGTGATCGACGAAGAGGAGGCGGTCAAGAACGCCGATTCCCAGAACAACCTGCGCCTCAA
GATCAAGCTGTGGAAGGAGAAGGGGCAGATAGGCAACAGTGCGGAGGCCAGCGGCTGGAGCCTGGAGCCCAGCAAGGATG
AGAACAACGATATCTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.667

98.945

0.541
  pilU Acinetobacter baylyi ADP1

52.394

93.668

0.491
  pilU Vibrio cholerae strain A1552

50

92.348

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.305

98.681

0.388
  pilT Pseudomonas aeruginosa PAK

41.57

90.765

0.377
  pilT Legionella pneumophila strain ERS1305867

42.515

88.127

0.375
  pilT Legionella pneumophila strain Lp02

42.515

88.127

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.171

92.612

0.372
  pilT Vibrio cholerae strain A1552

40.171

92.612

0.372
  pilT Pseudomonas stutzeri DSM 10701

40.988

90.765

0.372
  pilT Acinetobacter baumannii D1279779

41.916

88.127

0.369
  pilT Acinetobacter baumannii strain A118

41.916

88.127

0.369
  pilT Acinetobacter nosocomialis M2

41.916

88.127

0.369