Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrA   Type   Machinery gene
Locus tag   PYW35_RS00225 Genome accession   NZ_CP118974
Coordinates   46712..48061 (-) Length   449 a.a.
NCBI ID   WP_103323360.1    Uniprot ID   -
Organism   Mammaliicoccus vitulinus strain DSM 15615     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Genomic Context

Location: 41712..53061
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PYW35_RS00200 - 42188..43213 (-) 1026 WP_239102458.1 DNA adenine methylase -
  PYW35_RS00205 - 43316..43828 (-) 513 WP_103323364.1 DUF1643 domain-containing protein -
  PYW35_RS00210 - 43844..44155 (-) 312 WP_103323363.1 DUF960 family protein -
  PYW35_RS00215 - 44251..44589 (-) 339 WP_103323362.1 SAUGI family uracil-DNA glycosylase inhibitor -
  PYW35_RS00220 ccrB 45063..46691 (-) 1629 WP_103323361.1 cassette chromosome recombinase CcrB Machinery gene
  PYW35_RS00225 ccrA 46712..48061 (-) 1350 WP_103323360.1 cassette chromosome recombinase CcrA Machinery gene
  PYW35_RS00230 - 48253..48531 (-) 279 WP_031903869.1 hypothetical protein -
  PYW35_RS00235 cch1 48602..50392 (-) 1791 WP_103323359.1 cassette chromosome replicative helicase -
  PYW35_RS00240 ssb 50392..50685 (-) 294 WP_031863540.1 cassette chromosome ssDNA-binding protein -
  PYW35_RS00245 - 50857..52368 (-) 1512 WP_103323358.1 hypothetical protein -
  PYW35_RS00250 - 52528..52938 (-) 411 WP_199186561.1 hypothetical protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 52317.14 Da        Isoelectric Point: 10.1874

>NTDB_id=797032 PYW35_RS00225 WP_103323360.1 46712..48061(-) (ccrA) [Mammaliicoccus vitulinus strain DSM 15615]
MKQAMGYLRQSTTKQQSLPAQKQAIKTLAEKHNIQHITFYSDKQSGRTDKRNGYQQITELIQQGRCDVLCCYRLNRLHRN
LKNALKLMKLCQKYHVHILSVHDGYFDMDKAFDRLKLNIFISLAELESDNIGEQVSNGIKEKARQGKLITTHAPFGYHYK
RGTFTINKNESPTVIAVFNYYLQGNGYKKITQFLEEDNKLLNRKPYQVRNIIMNPNYCGRVINKYGQYDNMFPSIVSTNM
YEQAQALRSQKQIKRTPSDNQLKQKIKCPYCGSTLTNMTIRKKNHALRYYVCSRNMNASHFVCEFKGINAPKLETQVLTT
CQDFFQNQQLYLKINDVIQKRIKKQRALETRSTLTQEQLIDKLAKGAIDAETFRKQSQSLLQQSKPTLSINEQQIQRSFE
NVIQQHFTLSMLYRYIDEIHISKNKSLVGIYFKNEPLNIVNQATQSSIA

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=797032 PYW35_RS00225 WP_103323360.1 46712..48061(-) (ccrA) [Mammaliicoccus vitulinus strain DSM 15615]
ATGAAACAAGCAATGGGTTACTTACGTCAAAGTACAACAAAACAACAATCACTTCCGGCACAGAAACAAGCCATCAAGAC
ATTAGCTGAAAAACACAATATTCAACACATCACATTTTATAGCGATAAGCAATCAGGACGCACTGATAAGCGAAACGGTT
ACCAGCAAATTACTGAACTGATTCAACAAGGACGATGTGACGTACTATGTTGCTATCGTCTGAATCGACTTCATCGTAAT
CTAAAAAATGCATTAAAACTCATGAAATTGTGCCAAAAATATCATGTTCATATCTTAAGTGTTCATGATGGCTATTTTGA
TATGGATAAAGCATTCGATCGGCTTAAACTTAATATTTTCATCAGCTTGGCCGAATTAGAATCTGATAATATTGGCGAAC
AAGTAAGCAACGGGATAAAAGAAAAAGCGCGTCAGGGTAAATTGATAACTACACACGCCCCCTTTGGTTACCACTACAAA
CGTGGTACATTCACGATAAATAAGAATGAATCACCAACGGTAATTGCCGTATTCAATTACTATCTTCAAGGTAATGGCTA
TAAAAAAATTACACAGTTCTTAGAAGAAGATAACAAACTACTCAACCGGAAACCTTATCAAGTACGTAATATTATTATGA
ATCCCAATTACTGTGGTCGTGTTATCAATAAATATGGACAATATGACAACATGTTTCCGTCTATTGTTTCTACAAATATG
TACGAACAAGCGCAAGCTCTACGATCGCAAAAACAAATCAAACGAACACCTTCAGACAATCAACTCAAACAAAAAATCAA
ATGCCCTTATTGCGGGTCAACCCTTACCAATATGACAATTAGAAAAAAGAACCATGCATTACGTTACTATGTCTGTTCTC
GAAATATGAATGCATCTCATTTTGTCTGTGAATTCAAAGGGATTAATGCGCCAAAACTAGAAACTCAAGTCTTAACTACA
TGTCAGGACTTCTTTCAAAATCAACAACTTTACTTAAAAATCAACGATGTAATTCAAAAACGGATCAAAAAACAAAGGGC
CCTAGAAACTAGGAGCACACTTACACAAGAACAATTGATAGATAAATTAGCTAAAGGAGCCATCGATGCAGAAACGTTCA
GAAAACAATCTCAATCGTTACTTCAACAATCAAAACCAACACTATCAATAAATGAGCAACAAATTCAAAGGTCTTTTGAA
AATGTAATTCAACAACACTTCACGTTAAGCATGTTATACCGATATATTGATGAAATTCATATTTCTAAAAACAAAAGCCT
TGTGGGAATCTATTTCAAAAATGAACCGCTAAACATTGTAAACCAAGCTACGCAATCATCGATTGCTTAA

Domains

Predicted by InterproScan.

Resolvase, N terminal domain (Resolvase)

(4-147)

Recombinase (Recombinase)

(169-250)

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(262-322)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrA Staphylococcus aureus COL

79.955

100

0.8
  ccrA Staphylococcus aureus N315

75.278

100

0.753