Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   PYW33_RS01580 Genome accession   NZ_CP118963
Coordinates   368818..369876 (+) Length   352 a.a.
NCBI ID   WP_004644949.1    Uniprot ID   N9Q4M1
Organism   Acinetobacter lwoffii strain DSM 2403     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 363818..374876
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PYW33_RS01565 - 363872..364675 (+) 804 WP_004644947.1 putative RNA methyltransferase -
  PYW33_RS01570 - 364720..366024 (-) 1305 WP_004282307.1 IS4 family transposase -
  PYW33_RS01575 ponA 366127..368652 (-) 2526 WP_016806915.1 penicillin-binding protein PBP1a -
  PYW33_RS01580 pilM 368818..369876 (+) 1059 WP_004644949.1 pilus assembly protein PilM Machinery gene
  PYW33_RS01585 comN 369876..370514 (+) 639 WP_004281141.1 PilN domain-containing protein Machinery gene
  PYW33_RS01590 comO 370511..371233 (+) 723 WP_004644950.1 type 4a pilus biogenesis protein PilO Machinery gene
  PYW33_RS01595 pilP 371233..371760 (+) 528 WP_004644951.1 pilus assembly protein PilP Machinery gene
  PYW33_RS01600 pilQ 371779..373932 (+) 2154 WP_004644953.1 type IV pilus secretin PilQ family protein Machinery gene
  PYW33_RS01605 aroK 373969..374505 (+) 537 WP_004644955.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 39037.53 Da        Isoelectric Point: 4.5358

>NTDB_id=796908 PYW33_RS01580 WP_004644949.1 368818..369876(+) (pilM) [Acinetobacter lwoffii strain DSM 2403]
MRRLYRKPNKGLIGVDISSTSVKVLELSVKSGRYWVESYALIPLSENSVVEKNILNPEAVADALGRAINLGNTQSNQAAF
AIPTSMVITKIIEMDADMSDDEREVQIREDAEQYIPFPLDEASLDFEVLPDRLSNPNRVNVLLVATRIENVEARSEVLEI
SSLTPKIADVESFALENAFKVFSDTLPMGVNTVGILDIGHSMTTLSVMQNNKIIYTREQVFGGKQLTQEIQNRYGLSFEE
AGRAKKTRALPDDYDIEVLEPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQIDIKKIENDASSLMVACGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=796908 PYW33_RS01580 WP_004644949.1 368818..369876(+) (pilM) [Acinetobacter lwoffii strain DSM 2403]
GTGCGCAGGTTATATCGTAAACCAAATAAGGGGTTAATAGGAGTCGATATTAGTTCGACTTCTGTTAAGGTTTTAGAGCT
TTCTGTAAAAAGTGGCCGTTACTGGGTAGAAAGCTATGCTTTGATCCCATTATCGGAAAACAGTGTTGTTGAAAAAAACA
TCTTAAACCCAGAAGCAGTCGCTGATGCCCTTGGGCGTGCAATTAATTTGGGCAATACCCAGTCGAATCAGGCCGCTTTT
GCGATTCCTACTTCAATGGTCATCACCAAAATCATTGAGATGGATGCAGACATGTCAGATGATGAACGTGAAGTTCAGAT
TCGTGAAGATGCGGAACAATACATTCCTTTTCCACTCGATGAAGCGAGTCTGGACTTTGAAGTTCTGCCAGACCGCCTTT
CCAATCCGAACCGGGTCAATGTGCTGCTGGTCGCGACACGGATCGAAAATGTCGAAGCACGTTCTGAAGTTTTAGAGATT
AGCAGTCTAACGCCTAAGATTGCAGATGTAGAAAGTTTTGCGCTGGAAAATGCCTTTAAGGTATTTTCGGACACTTTGCC
GATGGGGGTCAATACGGTTGGTATTTTAGACATTGGTCATAGTATGACGACCTTGTCGGTGATGCAGAACAACAAGATTA
TTTATACCCGTGAGCAGGTGTTTGGTGGCAAGCAATTGACTCAGGAAATTCAGAATCGTTATGGATTGTCTTTTGAAGAA
GCTGGACGTGCTAAAAAAACCCGTGCCTTGCCGGATGATTATGATATTGAAGTGCTAGAACCATTTCTGGATGCCGTCGT
ACAACAGGCAGCCCGTTCATTGCAGTTCTTCTTTTCATCTTCTCAGTTTAATGAGATTGACCATATTTTACTGGCCGGCG
GGAATGCCAATATTCCAGGTCTGGCAAAATTATTACAACAAAAACTCGGTTACCGTGTCACGATCGCGAATCCATTTTTG
CAAATGGGCTTTTCTCCTCAAATTGATATTAAAAAAATTGAAAATGACGCGTCATCACTCATGGTTGCATGCGGTTTGGC
ATTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9Q4M1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

85.227

100

0.852
  comM Acinetobacter nosocomialis M2

85.227

100

0.852
  comM Acinetobacter baylyi ADP1

79.261

100

0.793
  pilM Legionella pneumophila strain ERS1305867

39.266

100

0.395