Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PWO50_RS02690 Genome accession   NZ_CP118914
Coordinates   595158..596198 (-) Length   346 a.a.
NCBI ID   WP_210797611.1    Uniprot ID   -
Organism   Pseudoalteromonas ostreae strain hOe-125     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 590158..601198
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWO50_RS02660 rsmE 590309..591043 (+) 735 WP_210797616.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PWO50_RS02665 gshB 591098..592051 (+) 954 WP_210797615.1 glutathione synthase -
  PWO50_RS02670 - 592079..592636 (+) 558 WP_210797614.1 YqgE/AlgH family protein -
  PWO50_RS02675 ruvX 592671..593117 (+) 447 WP_210797613.1 Holliday junction resolvase RuvX -
  PWO50_RS02680 - 593080..594006 (-) 927 WP_215909092.1 hypothetical protein -
  PWO50_RS02685 pilU 594008..595138 (-) 1131 WP_210797612.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PWO50_RS02690 pilT 595158..596198 (-) 1041 WP_210797611.1 type IV pilus twitching motility protein PilT Machinery gene
  PWO50_RS02695 - 596201..596914 (+) 714 WP_275074492.1 YggS family pyridoxal phosphate-dependent enzyme -
  PWO50_RS02700 proC 596930..597751 (+) 822 WP_210797609.1 pyrroline-5-carboxylate reductase -
  PWO50_RS02705 - 597809..598342 (+) 534 WP_210797608.1 YggT family protein -
  PWO50_RS02710 - 598394..598837 (+) 444 WP_210797607.1 DUF4426 domain-containing protein -
  PWO50_RS02715 - 598841..599440 (+) 600 WP_210797606.1 XTP/dITP diphosphatase -
  PWO50_RS02720 hemW 599437..600573 (+) 1137 WP_210797605.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38209.46 Da        Isoelectric Point: 6.4495

>NTDB_id=796284 PWO50_RS02690 WP_210797611.1 595158..596198(-) (pilT) [Pseudoalteromonas ostreae strain hOe-125]
MDITELLAFSVQHKASDLHLSSGVAPMIRVDGDVRRINIPALENKDVNSLVYDIMNDNQRKDYEQNLEVDFSFEVPNLAR
FRVNAFNSNRGPAAVFRTIPSEVLTLDDLGAPDIFKTISDTPRGLVLVTGPTGSGKSTTLAAMVDHINQHKHHHILTIED
PIEFVHDNKLSLINQREVHRDTHSFSNALRSALREDPDVILVGELRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
IDVFPGEEKSMIRSMLSESLRAVISQTLLKKIGGGRVAAHEIMIAVPAIRNLIREDKIAQMYSSIQTGASHGMQTMDQCL
TNLVNHGVVTNAEARAKAQDKNTFGS

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=796284 PWO50_RS02690 WP_210797611.1 595158..596198(-) (pilT) [Pseudoalteromonas ostreae strain hOe-125]
ATGGATATTACCGAATTGTTGGCCTTTAGTGTGCAGCACAAAGCATCAGATTTACACTTATCATCAGGGGTTGCGCCAAT
GATCCGTGTTGATGGTGATGTGCGACGTATTAATATACCAGCGCTGGAAAACAAAGATGTTAACAGCCTAGTTTACGATA
TTATGAACGATAATCAACGTAAGGACTACGAACAAAACCTAGAAGTGGATTTTTCGTTTGAAGTGCCAAACTTAGCGCGT
TTTCGTGTTAATGCCTTTAACTCTAATCGAGGCCCAGCTGCTGTATTTCGTACCATTCCTAGTGAAGTGCTGACACTTGA
TGATTTAGGTGCGCCAGATATATTTAAAACAATCTCAGATACGCCGCGTGGTTTAGTGTTGGTAACAGGACCTACAGGCT
CTGGTAAATCGACCACATTGGCTGCGATGGTGGATCATATAAATCAACATAAGCATCATCATATTTTAACCATTGAAGAC
CCTATTGAGTTTGTGCACGACAATAAGCTATCGTTGATTAATCAACGTGAAGTGCATCGCGATACGCATAGTTTTTCTAA
TGCACTACGCAGTGCTTTACGTGAAGATCCAGACGTGATCTTGGTTGGTGAGTTGCGTGATTTAGAAACTATTCGCCTAG
CTATGACCGCAGCTGAAACCGGCCATTTAGTATTTGGTACTTTGCATACCACCTCTGCGCCAAAAACCATTGACCGTATT
ATCGATGTATTCCCTGGAGAAGAAAAAAGCATGATCCGCTCTATGCTTTCAGAATCATTGCGTGCGGTAATTTCACAAAC
CCTGTTGAAAAAAATTGGTGGTGGGCGGGTCGCCGCCCATGAAATTATGATTGCAGTGCCTGCGATCAGAAACTTGATCC
GCGAAGATAAAATTGCACAAATGTATTCGTCGATTCAAACTGGTGCATCGCACGGCATGCAAACCATGGATCAATGTTTA
ACCAATTTAGTTAACCACGGTGTAGTTACCAATGCCGAGGCGCGCGCTAAAGCACAAGATAAAAATACTTTCGGCAGTTA
G

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

76.453

99.422

0.76
  pilT Acinetobacter baumannii D1279779

76.453

99.422

0.76
  pilT Acinetobacter baumannii strain A118

76.453

99.422

0.76
  pilT Acinetobacter baylyi ADP1

75.581

99.422

0.751
  pilT Pseudomonas stutzeri DSM 10701

75.581

99.422

0.751
  pilT Pseudomonas aeruginosa PAK

75.291

99.422

0.749
  pilT Legionella pneumophila strain Lp02

72.093

99.422

0.717
  pilT Legionella pneumophila strain ERS1305867

72.093

99.422

0.717
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.386

97.977

0.699
  pilT Vibrio cholerae strain A1552

71.386

97.977

0.699
  pilT Neisseria meningitidis 8013

68.986

99.711

0.688
  pilT Neisseria gonorrhoeae MS11

68.696

99.711

0.685
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

99.711

0.523
  pilU Vibrio cholerae strain A1552

40.816

99.133

0.405
  pilU Acinetobacter baylyi ADP1

39.595

100

0.396
  pilU Pseudomonas stutzeri DSM 10701

38.776

99.133

0.384