Detailed information    

insolico Bioinformatically predicted

Overview

Name   clpX   Type   Regulator
Locus tag   RAM32_RS12235 Genome accession   NZ_CP132361
Coordinates   2480154..2481416 (-) Length   420 a.a.
NCBI ID   WP_000472302.1    Uniprot ID   P63789
Organism   Staphylococcus aureus strain UNC_SA56     
Function   require for competence development (predicted from homology)   
Competence regulation

Genomic Context

Location: 2475154..2486416
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RAM32_RS12210 (RAM32_12200) - 2475352..2476020 (-) 669 WP_000805940.1 uroporphyrinogen-III synthase -
  RAM32_RS12215 (RAM32_12205) hemC 2476042..2476968 (-) 927 WP_001230228.1 hydroxymethylbilane synthase -
  RAM32_RS12220 (RAM32_12210) ccsA 2477010..2477825 (-) 816 WP_001157329.1 cytochrome c biogenesis protein -
  RAM32_RS12225 (RAM32_12215) hemA 2477847..2479193 (-) 1347 WP_000545451.1 glutamyl-tRNA reductase -
  RAM32_RS12230 (RAM32_12220) yihA 2479410..2480000 (-) 591 WP_000867697.1 ribosome biogenesis GTP-binding protein YihA/YsxC -
  RAM32_RS12235 (RAM32_12225) clpX 2480154..2481416 (-) 1263 WP_000472302.1 ATP-dependent Clp protease ATP-binding subunit ClpX Regulator
  RAM32_RS12240 (RAM32_12230) tig 2481567..2482868 (-) 1302 WP_153157918.1 trigger factor -
  RAM32_RS12245 (RAM32_12235) - 2482916..2483026 (-) 111 WP_001790560.1 hypothetical protein -
  RAM32_RS12250 (RAM32_12240) - 2483031..2483960 (-) 930 WP_001280018.1 hypothetical protein -
  RAM32_RS12255 (RAM32_12245) - 2483979..2484587 (-) 609 WP_000032655.1 NUDIX domain-containing protein -
  RAM32_RS12260 (RAM32_12250) rplT 2484729..2485085 (-) 357 WP_001138360.1 50S ribosomal protein L20 -
  RAM32_RS12265 (RAM32_12255) rpmI 2485132..2485332 (-) 201 WP_001125540.1 50S ribosomal protein L35 -
  RAM32_RS12270 (RAM32_12260) infC 2485361..2485888 (-) 528 WP_001791162.1 translation initiation factor IF-3 -

Sequence

Protein


Download         Length: 420 a.a.        Molecular weight: 46297.48 Da        Isoelectric Point: 4.2645

>NTDB_id=796135 RAM32_RS12235 WP_000472302.1 2480154..2481416(-) (clpX) [Staphylococcus aureus strain UNC_SA56]
MFKFNEDEENLKCSFCGKDQDQVKKLVAGSGVYICNECIELCSEIVEEELAQNTSEAMTELPTPKEIMDHLNEYVIGQEK
AKKSLAVAVYNHYKRIQQLGPKEDDVELQKSNIALIGPTGSGKTLLAQTLAKTLNVPFAIADATSLTEAGYVGDDVENIL
LRLIQAADFDIDKAEKGIIYVDEIDKIARKSENTSITRDVSGEGVQQALLKILEGTTASVPPQGGRKHPNQEMIQIDTTN
ILFILGGAFDGIEEVIKRRLGEKVIGFSSNEADKYDEQALLAQIRPEDLQAYGLIPEFIGRVPIVANLETLDVTALKNIL
TQPKNALVKQYTKMLELDDVDLEFTEEALSAISEKAIERKTGARGLRSIIEESLIDIMFDVPSNENVTKVVITAQTINEE
TEPELYDAEGNLINNSKTSA

Nucleotide


Download         Length: 1263 bp        

>NTDB_id=796135 RAM32_RS12235 WP_000472302.1 2480154..2481416(-) (clpX) [Staphylococcus aureus strain UNC_SA56]
ATGTTTAAATTCAATGAAGATGAAGAAAATTTGAAATGCTCTTTCTGCGGAAAAGACCAAGATCAAGTAAAAAAACTTGT
AGCAGGAAGTGGTGTATATATTTGTAATGAGTGTATTGAATTATGCTCAGAAATCGTCGAAGAAGAATTAGCTCAAAACA
CTTCTGAAGCGATGACAGAATTACCTACTCCTAAAGAAATTATGGATCATTTAAACGAATATGTTATTGGTCAAGAAAAA
GCTAAAAAATCTTTAGCTGTAGCTGTTTATAACCACTATAAGCGTATTCAACAATTAGGACCAAAAGAAGATGATGTTGA
ATTACAAAAAAGTAACATTGCATTAATTGGGCCAACAGGTAGTGGTAAAACATTATTAGCTCAAACATTAGCCAAGACGT
TGAATGTACCATTTGCAATTGCAGATGCGACAAGTTTAACTGAAGCTGGTTATGTAGGCGATGATGTTGAAAATATCTTG
TTGAGATTAATTCAAGCAGCTGACTTTGACATTGATAAAGCCGAAAAAGGTATTATTTATGTAGATGAAATTGATAAAAT
TGCACGTAAATCTGAAAACACATCTATAACACGTGACGTTTCAGGTGAAGGTGTTCAACAAGCATTGCTTAAAATCTTAG
AAGGTACGACTGCAAGTGTTCCGCCACAAGGTGGACGCAAACATCCAAACCAAGAAATGATTCAAATTGATACAACAAAT
ATCTTATTTATTCTTGGTGGTGCCTTTGATGGTATTGAAGAAGTGATTAAGCGCCGTCTTGGTGAAAAAGTTATTGGTTT
CTCAAGCAATGAAGCTGATAAATATGACGAACAAGCATTATTAGCACAAATTCGCCCAGAAGATTTGCAAGCCTATGGTT
TGATTCCTGAATTTATCGGACGTGTGCCAATTGTAGCTAATTTAGAAACATTAGATGTAACTGCGTTGAAAAACATCTTA
ACGCAACCTAAAAATGCACTTGTGAAACAATATACTAAAATGCTGGAATTAGATGATGTGGATTTAGAGTTCACTGAAGA
AGCTTTATCAGCAATTAGTGAAAAAGCAATTGAAAGAAAAACAGGTGCGCGTGGTTTACGTTCAATAATAGAAGAATCGT
TAATCGATATTATGTTTGATGTGCCTTCTAACGAAAATGTAACGAAGGTAGTTATTACAGCACAAACAATTAATGAAGAA
ACTGAACCAGAACTATACGACGCAGAAGGCAATTTAATTAATAATAGTAAAACATCAGCTTAA

Domains

Predicted by InterProScan.

C-terminal, D2-small domain, of ClpB protein (ClpB_D2-small)

(311-389)

AAA domain (Cdc48 subfamily) (AAA_2)

(109-305)

ClpX C4-type zinc finger (zf-C4_ClpX)

(11-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P63789

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  clpX Streptococcus mutans UA159

66.917

95

0.636
  clpX Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

56.456

94.048

0.531