Detailed information    

insolico Bioinformatically predicted

Overview

Name   comA   Type   Machinery gene
Locus tag   PYW04_RS09835 Genome accession   NZ_CP118874
Coordinates   2131367..2133580 (+) Length   737 a.a.
NCBI ID   WP_010952924.1    Uniprot ID   Q88LN2
Organism   Pseudomonas putida strain KT2440 Design 1a post-ALE     
Function   ssDNA transport through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2126367..2138580
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PYW04_RS09815 - 2128243..2128866 (+) 624 WP_010952921.1 glutathione S-transferase -
  PYW04_RS09820 - 2129000..2129932 (+) 933 WP_010952922.1 ABC transporter ATP-binding protein -
  PYW04_RS09825 - 2129929..2130708 (+) 780 WP_010952923.1 ABC transporter permease -
  PYW04_RS09830 - 2130718..2131224 (-) 507 Protein_1910 DUF2062 domain-containing protein -
  PYW04_RS09835 comA 2131367..2133580 (+) 2214 WP_010952924.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  PYW04_RS09840 exbB 2133691..2134326 (+) 636 WP_026031862.1 MotA/TolQ/ExbB proton channel family protein Machinery gene
  PYW04_RS09845 - 2134323..2134757 (+) 435 WP_010952926.1 biopolymer transporter ExbD -
  PYW04_RS09850 lpxK 2134757..2135758 (+) 1002 WP_049586866.1 tetraacyldisaccharide 4'-kinase -
  PYW04_RS09855 - 2135803..2135988 (+) 186 WP_003247142.1 Trm112 family protein -
  PYW04_RS09860 kdsB 2135985..2136749 (+) 765 WP_010952928.1 3-deoxy-manno-octulosonate cytidylyltransferase -
  PYW04_RS09865 - 2136749..2137213 (+) 465 WP_004575950.1 low molecular weight protein-tyrosine-phosphatase -
  PYW04_RS09870 murB 2137210..2138229 (+) 1020 WP_049586861.1 UDP-N-acetylmuramate dehydrogenase -

Sequence

Protein


Download         Length: 737 a.a.        Molecular weight: 79459.38 Da        Isoelectric Point: 10.4818

>NTDB_id=796044 PYW04_RS09835 WP_010952924.1 2131367..2133580(+) (comA) [Pseudomonas putida strain KT2440 Design 1a post-ALE]
MRTGMFALALGLLCLGFLPALPSVGWLITLAACAVGSLFTRVWPLGWFLLGLCWACGSAQQALDDRLATGLEGRTLWLEG
RVAGLPARTAHGVRFELEAPRSRRAELPQRLQLSWFDGAPLRAGEQWRLAVTLQRPNGLLNPHGPDREAQLLARRVGATG
TVKAGQLLAPVAGGWRDALRQRLLMVEANGRQAALVALVLGDGAGLAREDWQTLQATGTVHLLVISGQHIGLVAGLLYGL
VAGLARWGLWPARLPWLPWACGLAMAAALAYGWLAGGGVPVQRACLMLAVVLLWRLRFRHLGAFFPLLVALVAVLVFEPL
AALLPGFWLSFAAVATLIYCFSARLGGWRPWQAWTRAQWVIAVGLLPVLLATGLPVSLSAPLANLVAVPWVSLAVLPLAL
LGTLMLPLAGVGEALLWLAGGLLDVLFRGLALVAQQRPAWVPPALPLWAWLLVCLGVLLVLLPRGVPLRGLGGVMLLALW
VPREPVPFGQVEVWQLDVGQGLAVLLRTRHHSLLYDAGPARGESDLGERVVLPTLRKLGVGGLDVMVISHAHADHAGGAV
AIARGLPIKRIIGGEALVDVPLQPCASGEQWDWDGVRFSLWRWVDGQSSNDRSCVLLVEAQGERLLLAGDMEAAAERAWL
ADTEVPRIDWLQAPHHGSRSSSTEAFVRATAPRGVLISRGRNNSFGHPHVQVVERYGRHGVVMHDTAEEGALRLVLGRHG
VVEGVRGQRRFWRVRGE

Nucleotide


Download         Length: 2214 bp        

>NTDB_id=796044 PYW04_RS09835 WP_010952924.1 2131367..2133580(+) (comA) [Pseudomonas putida strain KT2440 Design 1a post-ALE]
ATGCGCACAGGGATGTTTGCGCTCGCGCTCGGGCTGTTGTGCCTGGGCTTTCTGCCCGCATTGCCATCGGTCGGATGGTT
GATAACCCTGGCAGCCTGCGCTGTCGGCAGCCTGTTTACCCGGGTCTGGCCGCTGGGTTGGTTTCTGCTGGGCTTGTGTT
GGGCGTGCGGGTCTGCTCAGCAGGCCCTTGATGATCGTCTGGCTACCGGCCTGGAGGGGCGCACCTTGTGGCTGGAGGGG
CGGGTGGCGGGTTTGCCGGCCCGCACTGCACACGGTGTGCGTTTCGAGCTGGAAGCGCCACGCTCGCGGCGGGCCGAACT
GCCTCAGCGCCTGCAGCTGAGCTGGTTCGACGGCGCGCCGCTGCGGGCGGGCGAGCAGTGGCGGCTGGCCGTGACCTTGC
AGCGCCCGAACGGGCTGCTGAACCCGCATGGGCCCGACCGTGAAGCGCAGTTGCTGGCGCGACGGGTGGGGGCTACGGGT
ACGGTCAAGGCGGGGCAGTTGCTGGCGCCAGTCGCCGGTGGTTGGCGCGATGCGCTGCGTCAGCGCTTGCTGATGGTCGA
AGCCAATGGCCGGCAGGCAGCGCTGGTGGCACTGGTGCTTGGCGACGGTGCGGGCCTGGCCCGTGAGGACTGGCAGACGT
TGCAGGCCACCGGCACGGTGCACCTGTTGGTGATTTCCGGCCAGCACATCGGCCTGGTCGCCGGTTTGCTCTATGGCCTG
GTCGCCGGGCTGGCGCGTTGGGGGCTATGGCCGGCAAGGCTACCGTGGTTACCCTGGGCCTGCGGCCTGGCCATGGCCGC
AGCGCTGGCCTACGGGTGGCTGGCTGGCGGTGGGGTGCCAGTGCAGCGGGCCTGCCTGATGCTGGCCGTGGTGTTGCTCT
GGCGTCTGCGCTTTCGCCACCTTGGCGCTTTTTTTCCGTTGCTGGTTGCACTGGTCGCTGTACTTGTCTTCGAGCCACTA
GCGGCGTTGCTGCCTGGGTTCTGGCTGTCGTTCGCCGCCGTGGCCACGCTCATCTACTGCTTCAGTGCCCGGTTGGGTGG
TTGGCGGCCCTGGCAGGCCTGGACACGAGCGCAATGGGTGATCGCCGTCGGCTTGCTACCGGTGTTGCTGGCCACCGGCT
TGCCGGTGAGCCTCAGTGCGCCGCTGGCCAACCTTGTGGCGGTGCCGTGGGTCAGCCTGGCGGTCTTGCCGTTGGCGTTG
CTGGGCACGCTGATGTTGCCGCTGGCGGGTGTTGGCGAGGCGTTGCTCTGGCTGGCAGGTGGCCTGCTGGATGTGCTGTT
CCGTGGGCTGGCGCTGGTGGCGCAGCAGCGCCCGGCATGGGTCCCGCCGGCCCTGCCGTTGTGGGCCTGGCTGCTGGTAT
GTCTGGGTGTGCTGTTGGTTTTGCTGCCCCGTGGTGTGCCGCTGCGCGGGTTGGGAGGGGTAATGCTGCTGGCGCTGTGG
GTGCCCCGGGAGCCGGTGCCGTTCGGCCAGGTCGAGGTCTGGCAGCTGGATGTTGGCCAGGGGCTGGCGGTGCTCTTGCG
TACGCGGCATCACAGCCTGCTCTACGATGCCGGGCCGGCCAGGGGGGAAAGCGACTTGGGGGAGCGAGTGGTGCTGCCAA
CCTTGCGCAAGCTGGGGGTGGGCGGCCTTGACGTGATGGTGATCAGCCATGCACATGCCGATCATGCGGGCGGGGCCGTG
GCCATTGCGCGTGGTTTGCCGATCAAGCGGATAATCGGTGGGGAAGCGCTGGTTGATGTTCCGTTGCAGCCTTGTGCCAG
TGGCGAGCAATGGGATTGGGATGGCGTGCGCTTTTCGCTGTGGCGCTGGGTGGACGGGCAGAGCAGTAATGACCGTTCCT
GTGTCTTGTTGGTCGAAGCGCAGGGCGAGCGCTTGCTGCTGGCGGGGGATATGGAAGCTGCTGCCGAAAGGGCCTGGCTG
GCGGACACTGAAGTGCCGCGTATCGACTGGCTGCAGGCGCCGCATCATGGCAGCCGCAGTTCGTCCACCGAGGCGTTCGT
CCGGGCTACCGCACCGCGCGGGGTATTGATTTCGCGGGGGCGCAACAACAGCTTCGGGCACCCGCACGTGCAGGTGGTCG
AGCGGTATGGGCGGCATGGGGTGGTGATGCATGATACGGCGGAGGAGGGGGCATTGCGGTTGGTGCTGGGGCGGCATGGG
GTAGTCGAGGGGGTTAGGGGGCAGCGGCGGTTCTGGCGGGTTCGAGGGGAGTGA

Domains

Predicted by InterproScan.

Domain of unknown function (DUF4131) (DUF4131)

(12-167)

Metallo-beta-lactamase superfamily (Lactamase_B)

(497-674)

Competence protein (Competence)

(198-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q88LN2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comA Pseudomonas stutzeri DSM 10701

58.587

97.965

0.574
  comA Ralstonia pseudosolanacearum GMI1000

34.821

100

0.37