Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   PYH50_RS06295 Genome accession   NZ_CP118837
Coordinates   1228847..1229743 (+) Length   298 a.a.
NCBI ID   WP_135031058.1    Uniprot ID   -
Organism   Mammaliicoccus lentus strain 7050     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1223847..1234743
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PYH50_RS06255 (PYH50_06255) - 1223903..1224457 (+) 555 WP_064204534.1 5-formyltetrahydrofolate cyclo-ligase -
  PYH50_RS06260 (PYH50_06260) - 1224458..1225906 (+) 1449 WP_239724181.1 rhomboid family intramembrane serine protease -
  PYH50_RS06265 (PYH50_06265) - 1225909..1226100 (+) 192 WP_017000229.1 YqgQ family protein -
  PYH50_RS06270 (PYH50_06270) - 1226097..1227068 (+) 972 WP_239724182.1 ROK family glucokinase -
  PYH50_RS06275 (PYH50_06275) - 1227068..1227382 (+) 315 WP_017000231.1 MTH1187 family thiamine-binding protein -
  PYH50_RS06280 (PYH50_06280) - 1227412..1227651 (-) 240 WP_017000232.1 DUF2759 domain-containing protein -
  PYH50_RS06285 (PYH50_06285) - 1227749..1228381 (+) 633 WP_017000233.1 MBL fold metallo-hydrolase -
  PYH50_RS06290 (PYH50_06290) - 1228446..1228694 (-) 249 WP_017000234.1 DUF2626 family protein -
  PYH50_RS06295 (PYH50_06295) comGA 1228847..1229743 (+) 897 WP_135031058.1 competence type IV pilus ATPase ComGA Machinery gene
  PYH50_RS06300 (PYH50_06300) comGB 1229740..1230780 (+) 1041 WP_239724184.1 competence type IV pilus assembly protein ComGB -
  PYH50_RS06305 (PYH50_06305) comGC 1230785..1231102 (+) 318 WP_070045385.1 competence type IV pilus major pilin ComGC Machinery gene
  PYH50_RS06310 (PYH50_06310) comGD 1231102..1231530 (+) 429 WP_017000239.1 competence type IV pilus minor pilin ComGD -
  PYH50_RS06315 (PYH50_06315) comGE 1231514..1231789 (+) 276 WP_017000240.1 competence type IV pilus minor pilin ComGE -
  PYH50_RS06320 (PYH50_06320) comGF 1231803..1232198 (+) 396 WP_239724188.1 competence type IV pilus minor pilin ComGF -
  PYH50_RS06325 (PYH50_06325) - 1232434..1232952 (+) 519 WP_064205405.1 shikimate kinase -
  PYH50_RS06330 (PYH50_06330) gcvT 1233115..1234203 (+) 1089 WP_103269518.1 glycine cleavage system aminomethyltransferase GcvT -

Sequence

Protein


Download         Length: 298 a.a.        Molecular weight: 34453.62 Da        Isoelectric Point: 5.8696

>NTDB_id=795760 PYH50_RS06295 WP_135031058.1 1228847..1229743(+) (comGA) [Mammaliicoccus lentus strain 7050]
METLFKTIIKDAIILKATDIHFIPVKEDRVKVQLRISGENTIYDCDINNALYNRLLIYMKFIAHLDVSERNKAQSGQYIF
DDIYKYYLRISTLPLSLGIESCSIRLTPKYFEEYIDHNIDDNYKMIKEEMFNSEGLFLFTGPTGSGKSTIMYQFLYDLQQ
IKDKHIITIEDPVEQVLDGVVQVSVNEKANINYHNSFKAILRCDPDVVMIGEIRDEETAKQVIQASLSGHLILSTMHAKD
NLGAIYRLLELGISKEQIKQGIACIANQRLLNDQLGNRYRQMTYIFSHEINLYLENKS

Nucleotide


Download         Length: 897 bp        

>NTDB_id=795760 PYH50_RS06295 WP_135031058.1 1228847..1229743(+) (comGA) [Mammaliicoccus lentus strain 7050]
ATGGAAACTTTATTTAAGACAATTATCAAAGATGCTATTATTTTGAAAGCAACGGACATTCATTTTATTCCAGTGAAAGA
GGATCGAGTCAAAGTTCAGTTGAGGATAAGTGGTGAGAATACGATTTATGATTGTGATATTAATAACGCTTTATATAACC
GTTTATTAATTTATATGAAGTTTATTGCTCATCTAGATGTAAGTGAAAGGAATAAAGCTCAAAGTGGTCAATATATATTT
GATGATATATATAAATATTATTTAAGAATATCCACTTTACCTTTAAGTCTAGGAATTGAGTCTTGCTCAATTAGGTTAAC
GCCTAAATATTTCGAAGAATATATTGACCATAATATTGATGACAATTATAAAATGATTAAAGAAGAAATGTTTAATTCCG
AAGGCTTATTTTTGTTCACAGGACCAACCGGTTCGGGTAAATCGACGATTATGTACCAATTTTTATATGACTTACAACAA
ATTAAAGATAAACATATTATTACGATTGAAGACCCAGTTGAACAAGTTTTAGATGGTGTAGTACAAGTTTCTGTAAATGA
AAAAGCAAACATAAATTATCATAATTCTTTTAAAGCCATTTTAAGATGTGATCCAGATGTTGTCATGATAGGAGAAATTA
GAGATGAAGAAACAGCCAAGCAAGTTATACAAGCAAGTTTAAGTGGTCATTTAATATTATCTACAATGCATGCGAAAGAT
AATTTAGGGGCTATTTATAGACTTTTAGAACTTGGAATATCTAAAGAGCAAATAAAACAAGGTATCGCATGTATAGCCAA
TCAAAGACTTTTAAATGACCAATTAGGAAATAGATATAGACAAATGACTTATATATTTAGTCATGAAATTAATCTATATT
TAGAAAATAAATCATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

50.993

100

0.517
  comGA Staphylococcus aureus N315

50.993

100

0.517