Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrB   Type   Machinery gene
Locus tag   PYH74_RS00165 Genome accession   NZ_CP118814
Coordinates   40886..42514 (-) Length   542 a.a.
NCBI ID   WP_061642462.1    Uniprot ID   -
Organism   Staphylococcus epidermidis strain 7060     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 41185..57183 40886..42514 flank -1329


Gene organization within MGE regions


Location: 40886..57183
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PYH74_RS00165 (PYH74_00165) ccrB 40886..42514 (-) 1629 WP_061642462.1 recombinase family protein Machinery gene
  PYH74_RS00170 (PYH74_00170) ccrA 42511..43872 (-) 1362 WP_061544228.1 recombinase family protein Machinery gene
  PYH74_RS00175 (PYH74_00175) - 44083..45849 (-) 1767 WP_061544227.1 DUF927 domain-containing protein -
  PYH74_RS00180 (PYH74_00180) - 45849..46142 (-) 294 WP_046464617.1 hypothetical protein -
  PYH74_RS00185 (PYH74_00185) - 46313..47827 (-) 1515 WP_046464618.1 hypothetical protein -
  PYH74_RS00190 (PYH74_00190) - 48458..49369 (+) 912 WP_046464619.1 ABC transporter substrate-binding protein -
  PYH74_RS00195 (PYH74_00195) - 49402..49572 (-) 171 WP_155734542.1 hypothetical protein -
  PYH74_RS00200 (PYH74_00200) - 49619..50293 (-) 675 WP_001106057.1 IS6-like element IS257 family transposase -
  PYH74_RS00205 (PYH74_00205) - 50377..50718 (+) 342 WP_001797647.1 hydroxymethylglutaryl-CoA synthase -
  PYH74_RS00210 (PYH74_00210) - 51635..52378 (+) 744 WP_000958858.1 glycerophosphoryl diester phosphodiesterase -
  PYH74_RS00215 (PYH74_00215) - 52475..52903 (+) 429 WP_000872606.1 MaoC family dehydratase -
  PYH74_RS00220 (PYH74_00220) mecA 52949..54958 (-) 2010 WP_103298748.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  PYH74_RS00225 (PYH74_00225) mecR1 55055..56812 (+) 1758 WP_000952923.1 beta-lactam sensor/signal transducer MecR1 -
  PYH74_RS00230 (PYH74_00230) mecI 56812..57183 (+) 372 WP_000369214.1 mecA-type methicillin resistance repressor MecI -

Sequence

Protein


Download         Length: 542 a.a.        Molecular weight: 62339.47 Da        Isoelectric Point: 9.5866

>NTDB_id=795517 PYH74_RS00165 WP_061642462.1 40886..42514(-) (ccrB) [Staphylococcus epidermidis strain 7060]
MTQLKKKRIGLYARVSTEMQTDGYSIHGQLNQLTEYCQFQGYEVVDEYTDRGISGKTTQRPELQRMLKDAKGGKLDGIMV
YNTTRLARNTSDLLNIVEELYEINVEFFSLTEKIEIASSTGKLMLQILASFAEFERNVIVENVFNGQRQRAIEGYYQGNL
PLGYDKVPESKKELMINQHEANIVKYIFESYAKGHGYRKIANTLNHKGYVTKKGKPFSISSITYIISNPFYIGKIQFAKY
RHWSDKRRKGLNEEPIIADGKHAPIIDKALWDKVQFKRQESRKKPQVHGKGTNLLTGIVKCPKCGAAMAASNTTNTLKDG
TKKRIRYYSCSNFRNKGSKVCSANSVRADVLEKYVMDQILEIIKSKKVLKQLVEKVNEQSQIDKSSLNHDIAYKQSQCEE
LKAKMHTLTKTIEDSPDLDSVLKPTILNYQDELNQINNQIHQLEQDKQAEAPHYDADMIANVLQAIFKDIDKLEKSQLKS
LYLTVIDRIDIRNHEHHKKQFYVTLKLNNEIIKQLFNNRPLDEVLLSTSSLFLPQTIYITIE

Nucleotide


Download         Length: 1629 bp        

>NTDB_id=795517 PYH74_RS00165 WP_061642462.1 40886..42514(-) (ccrB) [Staphylococcus epidermidis strain 7060]
ATGACACAACTCAAAAAGAAACGTATTGGATTATACGCACGTGTATCAACTGAAATGCAAACGGATGGTTACAGTATCCA
TGGTCAATTAAATCAACTTACAGAGTATTGTCAATTTCAAGGTTATGAAGTCGTTGATGAATATACCGATCGTGGTATAT
CAGGTAAAACAACGCAACGTCCTGAACTACAACGTATGTTAAAAGATGCTAAAGGAGGTAAATTAGATGGTATTATGGTA
TATAATACTACTCGTTTAGCTAGAAATACCTCAGATTTATTAAATATTGTCGAAGAATTGTATGAGATTAATGTTGAATT
TTTTAGTCTAACTGAAAAAATAGAAATTGCGAGCTCCACAGGTAAGTTGATGCTCCAAATCTTAGCGAGCTTCGCAGAAT
TTGAAAGAAACGTTATTGTAGAAAACGTGTTTAATGGCCAAAGACAACGTGCTATTGAAGGTTATTACCAAGGTAATTTA
CCTTTAGGCTATGATAAAGTACCTGAGAGTAAAAAAGAGCTTATGATTAATCAACATGAAGCAAATATTGTTAAATATAT
TTTTGAGTCATACGCCAAAGGTCACGGTTATCGTAAGATTGCGAACACATTGAATCATAAAGGTTATGTGACTAAGAAAG
GGAAACCGTTTAGTATTTCGTCCATTACTTATATCATCTCAAACCCTTTTTACATTGGAAAGATACAGTTTGCGAAGTAT
AGACACTGGAGCGATAAAAGACGAAAAGGACTCAATGAGGAACCGATTATCGCAGATGGTAAGCATGCGCCTATTATTGA
TAAAGCTTTATGGGATAAAGTACAGTTTAAACGCCAAGAGAGTCGCAAGAAACCACAAGTTCATGGTAAAGGGACCAATC
TACTTACTGGGATTGTAAAGTGCCCAAAATGTGGCGCAGCCATGGCGGCAAGTAATACGACCAATACATTAAAAGATGGC
ACTAAGAAAAGAATCAGATATTATTCATGTAGCAATTTTAGAAACAAAGGTTCTAAAGTATGTTCAGCCAATAGTGTTAG
AGCAGATGTATTAGAAAAATATGTTATGGACCAGATATTGGAAATTATCAAAAGTAAAAAGGTATTGAAGCAATTGGTTG
AAAAAGTAAATGAACAAAGCCAAATAGATAAATCTTCTTTGAATCATGATATTGCCTATAAACAGTCACAATGTGAAGAA
CTAAAAGCCAAAATGCATACATTAACGAAAACCATTGAAGACTCACCTGACTTAGATAGCGTATTGAAACCAACAATACT
TAACTATCAAGATGAATTGAATCAAATAAATAATCAAATCCATCAACTAGAGCAAGATAAACAAGCAGAAGCTCCACATT
ATGATGCTGATATGATTGCCAACGTACTACAAGCAATATTCAAAGATATAGACAAATTAGAGAAATCTCAGTTGAAATCA
CTCTATCTGACGGTTATCGATCGTATTGATATCCGAAACCATGAACATCATAAGAAGCAATTTTATGTTACGCTTAAACT
GAATAATGAAATTATCAAACAACTATTTAATAACCGTCCACTTGACGAAGTGCTCCTTAGCACTTCGTCTTTATTTTTGC
CACAAACTATATATATAACTATTGAATAA

Domains

Predicted by InterproScan.

Ogr/Delta-like zinc finger (Ogr_Delta)

(300-355)

Resolvase, N terminal domain (Resolvase)

(11-154)

Recombinase (Recombinase)

(179-280)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrB Staphylococcus aureus N315

72.14

100

0.721