Detailed information NTG0794421

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: PWN97_RS25695
• Genome accession: NZ_CP118691
• Coordinates: 5176367..5177167 (+)
• Length: 266 a.a.
• NCBI ID: WP_002888947.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain KPTCM2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5171367..5182167

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PWN97_RS25660 (PWN97_25660)	gspH	5171523..5172038 (+)	516	WP_004151939.1	type II secretion system minor pseudopilin GspH	-
PWN97_RS25665 (PWN97_25665)	gspI	5172035..5172400 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
PWN97_RS25670 (PWN97_25670)	gspJ	5172397..5172993 (+)	597	WP_004151940.1	type II secretion system minor pseudopilin GspJ	-
PWN97_RS25675 (PWN97_25675)	gspK	5172990..5173976 (+)	987	WP_004151941.1	type II secretion system minor pseudopilin GspK	-
PWN97_RS25680 (PWN97_25680)	gspL	5173963..5175156 (+)	1194	WP_004151942.1	type II secretion system protein GspL	-
PWN97_RS25685 (PWN97_25685)	-	5175149..5175634 (+)	486	WP_004151943.1	type II secretion system protein M	-
PWN97_RS25690 (PWN97_25690)	-	5175624..5176370 (+)	747	WP_004145897.1	type II secretion system protein N	-
PWN97_RS25695 (PWN97_25695)	pilD	5176367..5177167 (+)	801	WP_002888947.1	A24 family peptidase	Machinery gene
PWN97_RS25700 (PWN97_25700)	hrpB	5177215..5179644 (-)	2430	WP_004151944.1	ATP-dependent helicase HrpB	-
PWN97_RS25705 (PWN97_25705)	thpR	5179717..5180253 (+)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
PWN97_RS25710 (PWN97_25710)	sfsA	5180253..5180969 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
PWN97_RS25715 (PWN97_25715)	dksA	5181132..5181587 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29419.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=794421 PWN97_RS25695 WP_002888947.1 5176367..5177167(+) (pilD) [Klebsiella pneumoniae strain KPTCM2]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGSIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=794421 PWN97_RS25695 WP_002888947.1 5176367..5177167(+) (pilD) [Klebsiella pneumoniae strain KPTCM2]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTAGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGA
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGAGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425