Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   IGS56_RS03320 Genome accession   NZ_AP022846
Coordinates   662700..663239 (-) Length   179 a.a.
NCBI ID   WP_071162831.1    Uniprot ID   -
Organism   Haemophilus influenzae strain TAMBA230     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 657700..668239
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IGS56_RS03290 (JPHIA_06270) fdx 658106..658447 (+) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -
  IGS56_RS03295 (JPHIA_06280) iscX 658447..658641 (+) 195 WP_005659903.1 Fe-S cluster assembly protein IscX -
  IGS56_RS03300 (JPHIA_06290) - 658689..659303 (-) 615 WP_050838640.1 YfgM family protein -
  IGS56_RS03305 (JPHIA_06300) hisS 659321..660592 (-) 1272 WP_071162833.1 histidine--tRNA ligase -
  IGS56_RS03310 (JPHIA_06310) ispG 660602..661708 (-) 1107 WP_005654847.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  IGS56_RS03315 (JPHIA_06320) - 661717..662628 (-) 912 WP_071162832.1 helix-turn-helix domain-containing protein -
  IGS56_RS03320 (JPHIA_06330) pilF/pilF2 662700..663239 (-) 540 WP_071162831.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  IGS56_RS03325 (JPHIA_06340) - 663340..664491 (-) 1152 WP_042594886.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  IGS56_RS03330 (JPHIA_06350) - 664738..665616 (+) 879 WP_005656360.1 D-alanyl-D-alanine carboxypeptidase family protein -
  IGS56_RS03335 (JPHIA_06360) - 665981..666862 (+) 882 WP_071162830.1 metal ABC transporter substrate-binding protein -
  IGS56_RS03340 (JPHIA_06370) - 666862..667782 (+) 921 WP_040034370.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20623.26 Da        Isoelectric Point: 8.2571

>NTDB_id=79412 IGS56_RS03320 WP_071162831.1 662700..663239(-) (pilF/pilF2) [Haemophilus influenzae strain TAMBA230]
MKTINTQLSAVIFPFIFSACVSQSASNLNHQAAAKVRVELALSYLQQNNPQLAKINLDKALQHDKNYYLVHSALAHYYQQ
QGQIENAFREYEIAVNLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQADTFENIVLCAYSAQKMDIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=79412 IGS56_RS03320 WP_071162831.1 662700..663239(-) (pilF/pilF2) [Haemophilus influenzae strain TAMBA230]
ATGAAAACAATCAATACACAACTAAGTGCGGTTATTTTCCCTTTCATTTTTTCTGCTTGCGTTTCACAATCTGCATCTAA
CTTAAATCATCAAGCTGCCGCTAAAGTGCGTGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAATTTAGACAAAGCACTTCAACACGATAAAAATTACTATCTCGTGCATTCAGCACTTGCACATTATTATCAACAA
CAAGGGCAAATAGAAAATGCCTTTCGTGAGTATGAAATAGCCGTAAACCTTAATCATAAACAAGGCGATGTGCATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAACAAGCCCAGCAACAATTTGAATTAGCACTTAATTCGCCGAATT
ATTATCATCAAGCAGATACATTTGAAAATATTGTGCTTTGTGCTTATTCCGCGCAAAAAATGGATATTTATCAGCAAACA
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGTCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

97.207

100

0.972
  pilF Glaesserella parasuis strain SC1401

44.633

98.883

0.441

Multiple sequence alignment