Detailed information NTG0079328

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: KH172YL63_RS05870
• Genome accession: NZ_AP022842
• Coordinates: 1175040..1178801 (+)
• Length: 1253 a.a.
• NCBI ID: WP_173105224.1
• Uniprot ID: A0A6F8TQJ2
• Organism: Bacillus sp. KH172YL63
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1170040..1183801

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KH172YL63_RS05855 (KH172YL63_11120)	-	1170048..1170596 (+)	549	WP_173105221.1	GNAT family protein	-
KH172YL63_RS05860 (KH172YL63_11140)	-	1171036..1171422 (+)	387	WP_173105222.1	VOC family protein	-
KH172YL63_RS05865 (KH172YL63_11150)	addB	1171559..1175047 (+)	3489	WP_173105223.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
KH172YL63_RS05870 (KH172YL63_11160)	addA	1175040..1178801 (+)	3762	WP_173105224.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
KH172YL63_RS05875 (KH172YL63_11170)	-	1178985..1179287 (+)	303	WP_173105225.1	HNH endonuclease	-
KH172YL63_RS05880 (KH172YL63_11180)	-	1179449..1179667 (-)	219	WP_138777273.1	spore germination protein	-
KH172YL63_RS05885 (KH172YL63_11190)	-	1179751..1180158 (-)	408	WP_173105226.1	spore germination protein GerPE	-
KH172YL63_RS05890 (KH172YL63_11200)	-	1180162..1180341 (-)	180	WP_173105227.1	spore gernimation protein GerPD	-
KH172YL63_RS05895 (KH172YL63_11210)	gerPC	1180316..1180945 (-)	630	WP_173105228.1	spore germination protein GerPC	-
KH172YL63_RS05900 (KH172YL63_11220)	-	1180979..1181206 (-)	228	WP_173105229.1	spore germination protein GerPB	-
KH172YL63_RS05905 (KH172YL63_11230)	-	1181219..1181440 (-)	222	WP_173105230.1	spore germination protein	-
KH172YL63_RS05910 (KH172YL63_11240)	-	1181568..1182749 (+)	1182	WP_173105231.1	DUF418 domain-containing protein	-
KH172YL63_RS05915 (KH172YL63_11250)	-	1182774..1183643 (+)	870	WP_173105232.1	fumarylacetoacetate hydrolase family protein	-

Sequence

Protein

• Length: 1253 a.a.
• Molecular weight: 144181.86 Da
• Isoelectric Point: 4.9689

>NTDB_id=79328 KH172YL63_RS05870 WP_173105224.1 1175040..1178801(+) (addA) [Bacillus sp. KH172YL63]
MSKYVIPVKPDTMTWTDDQWKAIWAKGQDILVAAAAGSGKTAVLVERIIQKILNEEDPMDVDELLVVTFTNASAAEMRHR
IGQALEKAIDEDPHSHHLRKQLSLLNRASISTLHSFCLEVIRKYYYLIDIDPGFRIANETEGDLLRDEVLDDLFEEEYGK
EDNDDFFRLVDTFTSDRSDGALQDMIRKLYDFSRSHPNPDEWLRGLETMYEIGEGSEIDELNFIQPLLTDIQLQLHGAKN
LFQRAYELTRVPGGPGPRAENFLDDLALVESLEESRRESWSALYENIQTLAFTKLKPCRGADFNKEIVDEAKKLRDQGKK
ALEKLKEDFFSRRPQSFLDDMVKMKGVIHTLAEVVVEFGKRFKQVKEEKGLVDFADLEHFCLAILKDPASEELRPSEAAK
QYKQKFKEVLVDEYQDTNMVQESILLLITEEDEAIGNRFMVGDVKQSIYRFRLAEPNLFLGKYTRFLPESGEAGLKIDLS
QNFRSRKEVLEGTNFLFKQIMGISVGEMEYNKEAELVKGAPYPEEEAFPIEVALIDQESGEDASKDEELSIFDEGDIEKS
VLEARFMANKVRMLIDGRTPIYDAKTKTERPIQYRDIVILLRSMPWAAEIMEEFKRQGIPIYANLSTGYFEATEVAIMLS
LLKVIDNPYQDIPLASVLRSPVVGLDEQDLATVRIHSRSGSYYEAVKKFASEKPANPREEEAFEKIKVFLYHLQNWRTKA
RQGSVTELIWQLYRDTRFYDYVGGMAGGKQRQANLRALYDRARQYEETSFRGLFRFLRFIDRMRERGDDLGVARALSEQE
DVVRLMTIHSSKGLEFPVVFVAGTSKQFNLMDLNASYLLDKDFGLATKYTDPDKRISYPSLPQLAFKRKKRMESISEEMR
VLYVALTRAKEKLYLVGTVKSLDKSIEKWRGALGQTEWLLSDYDRAQASSYLDWIGPALMRHPHCGHLGEGAASTDSGIN
EEILLHPSCWKVTEIHKSELILEEDEEQSEELTWQEKVRKGLEVENSSEHKKGVLNRLSWKYPHLRATSLRSKQSVSELK
RMVEIRDEASSNEILRRHQKPVYNRPKFMQSKELSPAEKGTAMHTVMQHIPFAGGVPAREDVEELLSGLVHKEILTEEQQ
KAVSIDHIVGFFHSELGQRMVHAKEIQREIPFSMSVPLSEISETGEEAPEETILVQGVIDCVFRDEEGLVLLDYKTDGIH
DRYKDGFTEARPILEERYKVQIEYYTRALESIWKEPVSEKYLYFFDGGHILTL

Nucleotide

• Length: 3762 bp

>NTDB_id=79328 KH172YL63_RS05870 WP_173105224.1 1175040..1178801(+) (addA) [Bacillus sp. KH172YL63]
ATGAGTAAATATGTTATTCCAGTCAAACCTGACACTATGACGTGGACCGATGACCAATGGAAAGCGATATGGGCGAAAGG
GCAGGATATCCTTGTAGCCGCCGCGGCTGGTTCCGGTAAGACGGCTGTACTCGTGGAAAGGATCATCCAGAAGATCCTGA
ATGAAGAAGACCCGATGGATGTGGATGAACTGCTGGTGGTTACCTTTACGAATGCGTCTGCTGCCGAGATGAGGCACAGG
ATCGGACAGGCGTTGGAGAAAGCGATTGATGAAGATCCCCATTCACATCATCTGAGAAAGCAGCTGAGCCTGCTGAACCG
GGCGTCGATTTCCACCCTTCACTCCTTTTGTCTCGAAGTGATTCGGAAATATTATTATCTCATCGATATCGATCCCGGAT
TCAGGATCGCCAATGAGACCGAGGGGGACCTGCTCCGGGATGAAGTGCTTGATGACCTGTTTGAGGAGGAATACGGGAAA
GAAGATAATGATGATTTCTTCAGACTCGTCGATACGTTCACGAGCGATCGAAGTGACGGTGCCCTGCAGGATATGATCCG
TAAGCTGTATGATTTCTCCCGCTCCCATCCGAACCCGGATGAGTGGCTGAGAGGTCTTGAGACGATGTATGAAATCGGGG
AAGGGTCAGAGATTGATGAGCTTAACTTCATTCAGCCGTTATTGACGGATATTCAGCTGCAGCTGCACGGTGCGAAGAAC
CTGTTTCAGCGGGCATATGAATTGACCCGGGTGCCGGGAGGCCCTGGTCCGAGGGCGGAGAACTTCCTTGATGACCTGGC
CCTTGTGGAAAGCCTTGAGGAAAGCAGGCGTGAATCGTGGAGTGCTTTATATGAGAACATCCAAACCCTTGCATTCACTA
AACTGAAACCTTGCAGGGGGGCTGATTTCAATAAAGAAATCGTCGATGAAGCGAAAAAGCTCCGTGATCAGGGAAAGAAA
GCATTGGAAAAGCTGAAGGAAGATTTCTTCTCAAGAAGGCCACAGTCATTCCTGGATGACATGGTGAAGATGAAGGGGGT
CATTCACACCCTTGCAGAAGTGGTTGTCGAATTCGGGAAAAGGTTCAAGCAGGTGAAAGAAGAAAAAGGTCTGGTTGATT
TCGCCGATCTGGAGCATTTCTGCCTCGCCATCCTGAAAGATCCTGCATCTGAAGAACTGCGTCCGTCAGAAGCTGCGAAG
CAGTATAAACAAAAGTTCAAAGAAGTGCTGGTGGATGAATATCAGGATACGAACATGGTTCAGGAATCGATCCTCCTTCT
TATAACAGAAGAAGATGAAGCGATCGGCAACAGGTTCATGGTCGGGGATGTGAAACAGTCCATCTACCGATTCCGATTAG
CGGAACCGAACCTGTTTTTGGGCAAGTATACACGTTTCCTGCCTGAAAGCGGGGAAGCTGGGTTGAAGATCGATCTTTCC
CAGAACTTCCGGAGCCGTAAGGAAGTGTTGGAGGGGACGAACTTTTTATTCAAGCAGATCATGGGCATTTCTGTCGGGGA
GATGGAATATAACAAGGAAGCCGAACTTGTCAAAGGGGCACCTTATCCGGAAGAGGAGGCGTTTCCAATCGAAGTGGCCC
TGATCGATCAGGAGTCAGGTGAAGATGCTTCAAAGGATGAAGAGCTCTCGATCTTTGATGAAGGGGATATCGAGAAATCT
GTCCTTGAAGCCCGTTTTATGGCGAATAAAGTGCGGATGCTGATCGATGGCCGGACACCGATCTATGATGCGAAGACAAA
GACAGAACGTCCGATTCAGTATCGTGATATCGTCATACTGCTGAGATCAATGCCGTGGGCGGCTGAGATCATGGAAGAAT
TCAAACGGCAGGGAATCCCGATTTATGCAAATTTATCAACCGGTTATTTTGAAGCGACTGAGGTAGCGATCATGCTTTCA
TTATTGAAGGTGATCGATAATCCGTACCAGGATATTCCTCTTGCGTCGGTGCTCCGCTCTCCGGTTGTCGGTCTGGATGA
ACAGGACCTCGCCACTGTAAGGATCCATTCCCGGTCGGGCAGCTATTACGAAGCCGTCAAAAAATTCGCAAGTGAGAAAC
CGGCAAATCCGCGGGAAGAAGAAGCGTTCGAGAAGATCAAAGTATTCCTGTATCATTTACAGAACTGGCGGACAAAGGCG
CGACAGGGTTCCGTGACAGAATTGATCTGGCAGCTGTACCGCGATACCCGCTTCTATGATTATGTAGGGGGTATGGCCGG
CGGGAAGCAGCGGCAGGCAAACCTTCGTGCCCTGTATGACCGTGCACGCCAATACGAAGAAACGTCATTCAGGGGATTAT
TCCGCTTCCTGCGATTCATTGACCGTATGAGGGAAAGGGGCGATGACCTCGGGGTGGCAAGAGCCCTCAGCGAACAGGAA
GATGTGGTGCGTCTGATGACGATCCATTCGAGTAAAGGCCTGGAGTTTCCGGTCGTATTCGTCGCCGGGACTTCAAAGCA
ATTCAACCTGATGGACTTGAATGCTTCGTACCTTCTTGATAAAGATTTCGGTTTGGCTACAAAGTATACCGATCCGGATA
AACGAATCAGCTATCCGTCGCTGCCTCAACTTGCCTTTAAGCGAAAGAAGCGTATGGAGTCGATTTCTGAAGAAATGCGG
GTGCTGTATGTGGCATTGACCCGGGCAAAGGAGAAATTATACCTGGTCGGAACGGTGAAAAGCCTTGATAAATCCATTGA
AAAGTGGCGGGGTGCCCTCGGGCAGACGGAGTGGCTGCTGTCGGATTATGACCGGGCACAAGCAAGTTCGTATCTGGATT
GGATCGGTCCTGCCCTCATGCGCCACCCTCACTGCGGACATCTTGGTGAAGGGGCAGCGTCCACTGACTCAGGGATCAAC
GAGGAGATCCTTCTGCATCCTTCGTGCTGGAAAGTCACAGAGATCCACAAGTCTGAACTCATCTTAGAAGAAGATGAGGA
GCAGAGTGAAGAACTCACGTGGCAGGAGAAAGTGAGAAAAGGATTGGAAGTGGAAAATAGCTCTGAACATAAAAAAGGGG
TATTGAACCGCTTATCATGGAAGTATCCTCATCTCAGGGCAACGAGCCTGCGCTCAAAGCAATCGGTTTCCGAGCTGAAG
CGGATGGTGGAGATCCGGGACGAGGCATCCAGCAACGAAATATTGAGACGACACCAAAAACCTGTATACAACAGACCGAA
ATTCATGCAGTCGAAAGAGTTGTCACCGGCCGAAAAAGGGACTGCCATGCATACAGTCATGCAGCACATTCCGTTTGCGG
GTGGTGTGCCTGCAAGGGAAGATGTTGAAGAGCTGTTATCAGGATTGGTACATAAAGAAATCCTGACGGAAGAACAGCAG
AAGGCCGTTTCCATTGATCATATCGTCGGCTTCTTCCATAGTGAACTTGGTCAACGGATGGTCCATGCCAAAGAGATCCA
AAGGGAAATCCCGTTCAGTATGAGCGTTCCCCTCAGCGAGATATCAGAGACTGGAGAAGAGGCGCCGGAAGAAACGATCC
TCGTTCAAGGGGTCATCGACTGTGTTTTCCGGGATGAAGAAGGTCTGGTGCTTCTGGACTATAAGACTGATGGGATCCAC
GACCGTTACAAAGATGGCTTCACGGAAGCGAGGCCGATCCTTGAAGAGAGGTATAAAGTTCAAATCGAATACTATACACG
GGCACTGGAATCCATTTGGAAAGAGCCTGTCTCAGAGAAGTACTTATACTTCTTTGACGGAGGTCATATACTGACCTTGT
AA

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (477-896)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1067-1236)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (15-466)

3D structure

• AlphaFold DB: A0A6F8TQJ2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	56.709	99.92	0.567