Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   KH172YL63_RS05865 Genome accession   NZ_AP022842
Coordinates   1171559..1175047 (+) Length   1162 a.a.
NCBI ID   WP_173105223.1    Uniprot ID   A0A6F8TPC1
Organism   Bacillus sp. KH172YL63     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1166559..1180047
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KH172YL63_RS05840 (KH172YL63_11090) fabG 1166606..1167349 (+) 744 WP_173105219.1 3-oxoacyl-ACP reductase FabG -
  KH172YL63_RS05845 (KH172YL63_11100) - 1167376..1168551 (+) 1176 WP_173105220.1 thiolase family protein -
  KH172YL63_RS05850 (KH172YL63_11110) - 1168805..1169887 (+) 1083 WP_173108073.1 endonuclease -
  KH172YL63_RS05855 (KH172YL63_11120) - 1170048..1170596 (+) 549 WP_173105221.1 GNAT family protein -
  KH172YL63_RS05860 (KH172YL63_11140) - 1171036..1171422 (+) 387 WP_173105222.1 VOC family protein -
  KH172YL63_RS05865 (KH172YL63_11150) addB 1171559..1175047 (+) 3489 WP_173105223.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  KH172YL63_RS05870 (KH172YL63_11160) addA 1175040..1178801 (+) 3762 WP_173105224.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  KH172YL63_RS05875 (KH172YL63_11170) - 1178985..1179287 (+) 303 WP_173105225.1 HNH endonuclease -
  KH172YL63_RS05880 (KH172YL63_11180) - 1179449..1179667 (-) 219 WP_138777273.1 spore germination protein -

Sequence

Protein


Download         Length: 1162 a.a.        Molecular weight: 134464.46 Da        Isoelectric Point: 5.8984

>NTDB_id=79327 KH172YL63_RS05865 WP_173105223.1 1171559..1175047(+) (addB) [Bacillus sp. KH172YL63]
MSVRFILGRSGTGKTHRILDEMKQSLLAEPNGHPIIYIVPDQMSFISEYELVNTPGMNGMIRGQVFSLTRLAWKILQETG
GMSRYHLSDVGLNMMIRKIIDENKEDLHIFTKSSEKPGFIHHVEAMLTEFKRYCISPADLHQFDENGEREGSKVLKDKLH
DLELIYEKFEEKLSGKYIDSEDYFGLLAENVKNSALLKEAEIYIDGFYSFTPQEYLIIEQLILHAKNVTVALTTDPEQQM
GAMNELSLFRMTNETYQTLNDICRSGGVSVKEEVLVKPIRFHHETMLHLERHFEARPTVAFDKGETRIEFLEASNRRAEV
EGVAREIRKLAREEGYRYKDIAVLVRNSQDYRDKVETVFQDYEIPFFLDQKRTMLNHPLIELIRSTLEIMNTNWRYEPVF
RAVKTDLLFPYQKNPTDLREQMDRLENYVLAYGIKGDRWTKKDRWKYRRFRGLEHVDAPQTDQEREMEHEINELRLFISA
PIIRLGKRLKKQRTGREFCETLYLYLEELDIPAKLERLRIEAEDKGDLIAAREHDQAWSAVMDLLDQYVEMLGDEETTVK
KFSSILDAGMETMQFSLVPPAIDQVMIADLEKSRLGDIKAAFIIGANDGVLPAKMNEDGVLSDEDRESLISRGFKIAPGN
RTKLLDEEFVAYKAFTTPSERLFISYPIANEEGKALLPSSFIKRMNEMYPDAHTKLLINEPSELPEEEQLHYVCHPNVTI
SHLTSQLQMKKRDYPIYDFWWDVYNFYSTSNDWKWHSQHILSSLFYQNRADQLSEDTSRELYGDHILASVSRMELFHSCP
FSHFASHGLKLRERDFFKLEAPDIGEMFHSALKWMSEEVERHGLTWASLTKAQCIQLAKEAVLMLAPKLQHQILLSSNRH
HYIAKKLEQIISRAGLILSEHAKASGFVPVGVELGFGPRATLPPFQFQLRNGTKMELQGRIDRVDKAEDENGVYLRIVDY
KSSARDLDMTEVYYGLALQMLTYLDIVLTNSKKFVGKEAKPAGVLYFHVHNPMINSKKILTLDQIEEEIFKSFKMKGMVL
GDAGIVRLMDQTLETGNSDIISAGIKKDGSLSSRSKAASHEDFQYMRQHVRKLYETSGNRIVSGETDISPYKLKDRTPCQ
FCSYRSVCQFDQSLEENDYRVLPTAKPEDLLAKVREEVETDE

Nucleotide


Download         Length: 3489 bp        

>NTDB_id=79327 KH172YL63_RS05865 WP_173105223.1 1171559..1175047(+) (addB) [Bacillus sp. KH172YL63]
ATGAGTGTGCGATTTATTCTCGGACGCTCCGGTACCGGGAAAACCCATCGGATTTTGGATGAGATGAAGCAAAGCTTATT
GGCAGAGCCAAATGGACATCCCATCATTTATATTGTACCGGATCAAATGTCCTTTATTTCGGAATACGAGCTTGTGAACA
CGCCTGGGATGAACGGGATGATCCGTGGCCAGGTGTTCAGCCTCACGCGCCTTGCATGGAAAATCCTTCAGGAAACTGGC
GGCATGAGCCGGTATCATTTATCGGATGTTGGATTGAATATGATGATCCGGAAGATCATTGACGAAAACAAAGAGGATCT
TCATATTTTTACGAAGTCTTCGGAGAAGCCCGGCTTCATTCACCATGTGGAAGCGATGTTGACTGAATTCAAGCGCTACT
GCATCTCACCGGCGGACCTCCACCAGTTCGATGAGAATGGTGAAAGGGAAGGGTCCAAGGTGCTGAAGGATAAGCTTCAT
GACCTTGAATTGATTTATGAGAAATTTGAGGAGAAGCTTTCAGGAAAGTACATTGATTCAGAAGACTATTTTGGCCTGCT
TGCTGAAAATGTGAAGAATTCAGCGCTCCTGAAAGAAGCGGAAATATACATAGACGGCTTTTACAGCTTCACGCCACAGG
AATATCTCATCATCGAACAGCTCATCCTGCACGCAAAAAATGTGACGGTCGCCCTTACAACCGATCCTGAACAGCAGATG
GGTGCGATGAACGAATTAAGCTTATTCCGCATGACGAATGAAACCTATCAAACGCTTAACGACATATGCAGGAGCGGCGG
GGTTTCGGTGAAAGAAGAAGTGCTCGTCAAACCGATCCGCTTCCATCATGAAACGATGCTCCATTTAGAGCGTCACTTTG
AAGCGCGTCCAACCGTCGCTTTTGATAAAGGGGAGACGAGGATTGAATTTCTGGAAGCATCCAACCGGCGGGCCGAAGTG
GAAGGGGTCGCCCGTGAGATCAGGAAGCTTGCCAGGGAGGAAGGGTATCGCTATAAAGATATCGCCGTATTGGTGAGGAA
CAGCCAGGACTACCGTGACAAAGTCGAGACGGTGTTTCAAGATTATGAGATTCCATTTTTCCTTGATCAAAAGAGGACGA
TGCTGAATCATCCGCTGATTGAACTGATCCGCTCGACACTTGAAATCATGAATACGAACTGGAGATACGAGCCAGTGTTC
CGGGCAGTGAAAACAGATCTATTATTCCCTTATCAGAAGAACCCGACCGATCTCCGTGAGCAGATGGACAGGCTGGAAAA
CTATGTGCTGGCCTACGGGATCAAGGGTGATCGCTGGACGAAGAAGGATCGTTGGAAATACCGCCGCTTCCGGGGACTCG
AGCATGTCGATGCCCCGCAGACGGATCAGGAACGGGAAATGGAGCATGAAATCAATGAACTGAGATTATTTATTTCTGCA
CCGATCATCCGGCTTGGAAAACGGCTCAAAAAGCAGAGGACAGGAAGAGAATTCTGTGAAACACTCTATCTGTATCTGGA
AGAGCTTGATATACCGGCAAAGCTTGAGCGACTCCGGATCGAGGCAGAGGATAAAGGCGATCTGATTGCTGCAAGGGAGC
ATGATCAGGCGTGGTCAGCCGTCATGGATTTGCTTGATCAGTATGTAGAGATGCTTGGTGACGAGGAAACGACCGTGAAG
AAGTTTTCGTCGATCCTTGATGCAGGGATGGAAACGATGCAGTTTTCCCTTGTGCCGCCGGCTATCGATCAGGTCATGAT
CGCCGACTTGGAAAAATCAAGGCTCGGGGATATTAAAGCGGCTTTCATCATCGGAGCGAACGACGGTGTGCTTCCTGCGA
AGATGAATGAAGACGGGGTCCTGTCTGATGAGGACCGTGAATCATTGATCAGTAGGGGATTCAAGATTGCCCCCGGCAAC
AGGACGAAGCTTCTTGATGAAGAGTTTGTTGCATATAAAGCTTTTACGACTCCGAGTGAACGCTTATTTATCAGCTATCC
GATCGCGAATGAAGAAGGCAAAGCTTTACTTCCTTCCTCTTTCATTAAAAGGATGAACGAAATGTACCCGGATGCTCATA
CGAAGCTTCTCATCAATGAACCTTCCGAGCTGCCTGAAGAGGAACAGCTGCATTATGTGTGTCATCCGAATGTGACGATT
TCCCATTTGACGTCACAACTTCAGATGAAGAAAAGGGACTACCCAATATATGATTTCTGGTGGGATGTATATAACTTCTA
TTCAACGTCAAACGACTGGAAATGGCATTCACAGCATATTTTATCAAGCCTGTTTTACCAAAATCGTGCCGATCAGCTGT
CTGAGGACACGAGCAGGGAACTGTATGGAGATCATATCCTGGCAAGTGTCTCCCGGATGGAACTGTTCCACAGCTGTCCG
TTTTCCCATTTCGCAAGCCACGGGCTGAAGCTGAGGGAGCGGGATTTCTTTAAGCTGGAAGCACCTGATATCGGCGAAAT
GTTCCACTCGGCGTTGAAATGGATGTCTGAAGAAGTGGAGCGGCACGGATTGACCTGGGCATCGCTTACGAAAGCACAGT
GTATCCAGCTTGCGAAAGAAGCGGTCCTGATGCTCGCGCCGAAGCTGCAGCATCAGATTCTCCTAAGCTCGAACCGGCAT
CATTACATTGCCAAAAAGCTTGAACAGATCATCAGCCGGGCGGGCCTGATCCTGAGTGAACATGCAAAGGCAAGCGGCTT
TGTCCCGGTCGGGGTGGAGCTTGGGTTCGGACCCCGTGCGACCCTCCCGCCGTTCCAATTTCAGTTGAGAAACGGGACGA
AGATGGAGCTTCAAGGAAGGATCGACCGTGTGGATAAAGCGGAAGATGAAAACGGGGTATACCTCAGAATCGTCGATTAC
AAGTCGAGTGCGAGAGATCTGGATATGACGGAAGTATATTATGGATTGGCGCTGCAGATGCTGACGTACCTTGATATCGT
GCTGACGAATTCCAAGAAGTTTGTCGGGAAGGAAGCGAAGCCTGCAGGGGTACTCTATTTCCATGTTCATAATCCTATGA
TCAACAGTAAGAAAATCCTGACGCTTGATCAAATCGAGGAGGAAATCTTTAAGAGCTTCAAGATGAAAGGTATGGTTCTT
GGGGACGCCGGGATCGTCCGCTTGATGGATCAAACGCTGGAGACGGGTAATTCCGATATCATCTCAGCGGGAATTAAGAA
GGACGGTTCACTGTCCTCCCGTTCAAAAGCTGCAAGCCATGAAGATTTTCAATATATGCGCCAGCATGTGCGCAAGCTGT
ACGAAACATCCGGCAACAGAATCGTTTCAGGGGAGACGGACATTTCTCCTTATAAGCTGAAGGACCGTACACCTTGTCAA
TTCTGTTCTTACCGTTCTGTCTGTCAGTTCGATCAATCCCTTGAGGAAAATGACTATAGGGTATTGCCGACAGCGAAGCC
GGAAGATTTATTAGCGAAAGTGAGAGAGGAGGTAGAAACAGATGAGTAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(788-1129)

UvrD-like helicase C-terminal domain (UvrD_C)

(300-666)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6F8TPC1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

52.356

100

0.526

Multiple sequence alignment