Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   PWA59_RS18510 Genome accession   NZ_CP118541
Coordinates   3683901..3685286 (-) Length   461 a.a.
NCBI ID   WP_014419012.1    Uniprot ID   I2CAS2
Organism   Bacillus velezensis strain HC-5     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3678901..3690286
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWA59_RS18475 (PWA59_18475) flgL 3679076..3679993 (-) 918 WP_014419007.1 flagellar hook-associated protein FlgL -
  PWA59_RS18480 (PWA59_18480) flgK 3680005..3681522 (-) 1518 WP_014419008.1 flagellar hook-associated protein FlgK -
  PWA59_RS18485 (PWA59_18485) - 3681538..3682020 (-) 483 WP_003151394.1 flagellar protein FlgN -
  PWA59_RS18490 (PWA59_18490) flgM 3682035..3682301 (-) 267 WP_003151393.1 flagellar biosynthesis anti-sigma factor FlgM -
  PWA59_RS18495 (PWA59_18495) - 3682371..3682790 (-) 420 WP_014419009.1 TIGR03826 family flagellar region protein -
  PWA59_RS18500 (PWA59_18500) comFC 3682864..3683553 (-) 690 WP_197535984.1 ComF family protein Machinery gene
  PWA59_RS18505 (PWA59_18505) - 3683559..3683843 (-) 285 WP_014419011.1 competence protein ComFB -
  PWA59_RS18510 (PWA59_18510) comFA 3683901..3685286 (-) 1386 WP_014419012.1 DEAD/DEAH box helicase Machinery gene
  PWA59_RS18515 (PWA59_18515) - 3685394..3686242 (-) 849 WP_003151387.1 DegV family protein -
  PWA59_RS18520 (PWA59_18520) degU 3686340..3687029 (-) 690 WP_111927758.1 two-component system response regulator DegU Regulator
  PWA59_RS18525 (PWA59_18525) degS 3687106..3688269 (-) 1164 WP_007407484.1 two-component sensor histidine kinase DegS Regulator
  PWA59_RS18530 (PWA59_18530) - 3688492..3689139 (+) 648 WP_007614107.1 YigZ family protein -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52599.04 Da        Isoelectric Point: 10.2804

>NTDB_id=793133 PWA59_RS18510 WP_014419012.1 3683901..3685286(-) (comFA) [Bacillus velezensis strain HC-5]
MSEGASEFSEVRDFFYGRHLLRSEIPFSDQRIKRLTEKEYITAEPSIIRRKNRYVCQRCGQSDQASFAAFWAPSAKRQIT
YCRACVMMGRADELTSLYSWNQAPENSWEPVKLSWEGTLTDGQKRAAAALTDAIKERQELLVWAVCGSGKTEMLFPGIEF
ALNHGLRVCVATPRTDVVLELLPRLNKAFEKVEVSALYGGSEDKGRLTPLMISTAHQLMRYRDIFDVMIIDEVDAFPFSA
DETLRFAVDKARKKNSALVYVTATPSDTLKKKAEAGLLKSVRIPARYHRKPLPEPRFLWCGNWKKKLQKGKLPRSVTDWV
RQKLQLQLPVFLFVPSVHVLKKTTDYFRKLNIRAEGVHAEDTFRKDKVKRFRDGRLDLLVTTTILERGVTVPKVQTCVLG
AEAPIFTESALVQIAGRTGRHYKHFSGDVVMFHFGITNGMKKAKKHIEHMNKLAQKSKLLD

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=793133 PWA59_RS18510 WP_014419012.1 3683901..3685286(-) (comFA) [Bacillus velezensis strain HC-5]
ATGTCCGAAGGTGCTTCAGAATTTTCAGAGGTGCGGGATTTCTTTTACGGCCGGCATTTATTAAGATCTGAGATACCGTT
TTCAGACCAGCGCATCAAGCGTTTAACAGAAAAAGAATATATAACGGCCGAGCCGTCAATCATCCGCCGGAAAAACCGTT
ACGTCTGCCAACGATGCGGTCAGTCTGATCAAGCCAGCTTTGCGGCATTTTGGGCGCCCTCTGCCAAAAGGCAGATTACC
TATTGCCGCGCCTGTGTCATGATGGGAAGGGCTGATGAACTGACATCATTATATTCTTGGAATCAAGCCCCTGAAAACAG
CTGGGAGCCTGTAAAGCTTTCTTGGGAAGGAACACTGACAGACGGACAGAAGCGGGCGGCCGCCGCGCTTACAGATGCGA
TAAAAGAGAGACAAGAGCTGTTAGTATGGGCGGTTTGCGGATCGGGGAAAACAGAGATGCTGTTTCCGGGTATCGAGTTT
GCGCTGAATCACGGTTTGCGCGTTTGTGTCGCAACTCCGCGTACAGATGTCGTACTTGAACTGCTGCCGAGACTGAATAA
AGCATTTGAGAAAGTCGAAGTTTCTGCTTTGTACGGCGGCAGCGAGGACAAAGGCCGCTTGACTCCGCTGATGATTTCAA
CCGCTCACCAGCTGATGCGCTACCGAGACATTTTTGATGTCATGATAATAGATGAAGTAGATGCGTTTCCTTTTTCAGCC
GATGAGACATTAAGATTCGCTGTTGATAAAGCGAGGAAGAAAAACAGCGCCCTTGTTTATGTAACGGCGACTCCTTCAGA
CACATTAAAAAAGAAGGCTGAAGCCGGTTTGCTGAAAAGCGTCCGCATACCGGCCAGATATCATCGGAAACCCCTCCCGG
AGCCGCGTTTTTTGTGGTGCGGCAATTGGAAGAAGAAGCTTCAGAAAGGAAAACTGCCGCGCTCTGTAACGGATTGGGTC
CGCCAAAAACTTCAATTACAGCTGCCGGTTTTCTTATTCGTCCCGTCTGTACACGTATTGAAAAAGACAACGGATTATTT
TCGGAAATTAAACATACGGGCAGAAGGCGTACATGCGGAAGACACATTCAGAAAGGATAAAGTGAAACGATTCAGAGACG
GCCGGCTTGACCTGCTGGTAACGACTACGATTTTAGAAAGAGGGGTTACCGTGCCCAAAGTTCAGACATGTGTCCTCGGC
GCAGAAGCACCTATCTTTACTGAGAGCGCGCTCGTACAGATCGCGGGCAGAACGGGAAGGCATTATAAACATTTCAGCGG
TGATGTCGTCATGTTTCATTTCGGAATTACAAACGGAATGAAAAAAGCAAAAAAACATATAGAACATATGAACAAATTGG
CACAAAAAAGTAAATTGTTGGACTAG

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(118-266)

Helicase conserved C-terminal domain (Helicase_C)

(330-421)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I2CAS2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

62.391

99.783

0.623