Detailed information NTG0792442

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: PWK27_RS21580
• Genome accession: NZ_CP118398
• Coordinates: 4326608..4327408 (+)
• Length: 266 a.a.
• NCBI ID: WP_019705879.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain 2020CK-00223
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4321608..4332408

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PWK27_RS21545 (PWK27_21545)	gspH	4321764..4322273 (+)	510	WP_104159366.1	type II secretion system minor pseudopilin GspH	-
PWK27_RS21550 (PWK27_21550)	gspI	4322276..4322641 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
PWK27_RS21555 (PWK27_21555)	gspJ	4322638..4323234 (+)	597	WP_040237569.1	type II secretion system minor pseudopilin GspJ	-
PWK27_RS21560 (PWK27_21560)	gspK	4323231..4324217 (+)	987	WP_023341040.1	type II secretion system minor pseudopilin GspK	-
PWK27_RS21565 (PWK27_21565)	gspL	4324204..4325397 (+)	1194	WP_109256684.1	type II secretion system protein GspL	-
PWK27_RS21570 (PWK27_21570)	-	4325390..4325875 (+)	486	WP_004151943.1	type II secretion system protein M	-
PWK27_RS21575 (PWK27_21575)	-	4325865..4326611 (+)	747	WP_004145897.1	type II secretion system protein N	-
PWK27_RS21580 (PWK27_21580)	pilD	4326608..4327408 (+)	801	WP_019705879.1	A24 family peptidase	Machinery gene
PWK27_RS21585 (PWK27_21585)	hrpB	4327456..4329885 (-)	2430	WP_104158838.1	ATP-dependent helicase HrpB	-
PWK27_RS21590 (PWK27_21590)	thpR	4329958..4330494 (+)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
PWK27_RS21595 (PWK27_21595)	sfsA	4330494..4331210 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
PWK27_RS21600 (PWK27_21600)	dksA	4331373..4331828 (+)	456	WP_274759302.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29435.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=792442 PWK27_RS21580 WP_019705879.1 4326608..4327408(+) (pilD) [Klebsiella pneumoniae strain 2020CK-00223]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPISWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGSIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=792442 PWK27_RS21580 WP_019705879.1 4326608..4327408(+) (pilD) [Klebsiella pneumoniae strain 2020CK-00223]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCTCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTAGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGA
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGAGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425