Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   PUW21_RS19275 Genome accession   NZ_CP118167
Coordinates   3674839..3676230 (-) Length   463 a.a.
NCBI ID   WP_274358131.1    Uniprot ID   -
Organism   Bacillus subtilis strain 21855     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3669839..3681230
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUW21_RS19240 (PUW21_19240) flgL 3670010..3670906 (-) 897 WP_003228004.1 flagellar hook-associated protein FlgL -
  PUW21_RS19245 (PUW21_19245) flgK 3670917..3672440 (-) 1524 WP_003228001.1 flagellar hook-associated protein FlgK -
  PUW21_RS19250 (PUW21_19250) flgN 3672459..3672941 (-) 483 WP_003227999.1 flagellar protein FlgN -
  PUW21_RS19255 (PUW21_19255) flgM 3672957..3673223 (-) 267 WP_003227997.1 flagellar biosynthesis anti-sigma factor FlgM -
  PUW21_RS19260 (PUW21_19260) yvyF 3673304..3673723 (-) 420 WP_003227995.1 TIGR03826 family flagellar region protein -
  PUW21_RS19265 (PUW21_19265) comFC 3673797..3674519 (-) 723 WP_003227991.1 comF operon protein ComFC Machinery gene
  PUW21_RS19270 (PUW21_19270) comFB 3674483..3674779 (-) 297 WP_003227989.1 late competence protein ComFB -
  PUW21_RS19275 (PUW21_19275) comFA 3674839..3676230 (-) 1392 WP_274358131.1 ATP-dependent helicase ComFA Machinery gene
  PUW21_RS19280 (PUW21_19280) fakBA 3676336..3677181 (-) 846 WP_003244125.1 DegV family protein -
  PUW21_RS19285 (PUW21_19285) degU 3677279..3677968 (-) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  PUW21_RS19290 (PUW21_19290) degS 3678051..3679208 (-) 1158 WP_003227983.1 two-component sensor histidine kinase DegS Regulator
  PUW21_RS19295 (PUW21_19295) yvyE 3679425..3680078 (+) 654 WP_003227979.1 YigZ family protein -

Sequence

Protein


Download         Length: 463 a.a.        Molecular weight: 52562.61 Da        Isoelectric Point: 10.0512

>NTDB_id=791004 PUW21_RS19275 WP_274358131.1 3674839..3676230(-) (comFA) [Bacillus subtilis strain 21855]
MNVPVEKNSSFSKELQQTLRSRHLLRTELSFSDEMIEWHIKNGYITAENSISINKRRYRCNRCGQTDQRYFSFYHSSGKN
KLYCRSCVMMGRVSEEVPLYSWKEENESNWKSIKLTWDGKLSSGQQKAANVLIEAISKKEELLIWAVCGAGKTEMLFPGI
ESALNQGLRVCIATPRTDVVLELAPRLKAAFQGADISALYGGSDDKGRLSPLMISTTHQLLRYKDAIDVMIIDEVDAFPY
SADQTLQFAVQKARKKNSTLVYLSATPPKELKRKALNGQLHSVRIPARHHRKPLPEPRFVWCGNWKKKLNRNKIPPAVKR
WIEFHVKEGRPVFLFVPSVSILEKAAACFKGVHCRTASVHAEDKHRKEKVQQFRDGQLDLLITTTILERGVTVPKVQTGV
LGAESSIFTESALVQIAGRTGRHKEYADGDVIYFHFGKTKSMLDARKHIKEKNELAAKVECTD

Nucleotide


Download         Length: 1392 bp        

>NTDB_id=791004 PUW21_RS19275 WP_274358131.1 3674839..3676230(-) (comFA) [Bacillus subtilis strain 21855]
GTGAATGTGCCAGTTGAAAAAAACAGTTCCTTTTCAAAAGAATTGCAGCAGACGCTTCGAAGCCGTCATTTGCTCAGGAC
TGAGCTCTCATTTTCCGATGAGATGATTGAATGGCATATCAAGAATGGATATATCACTGCTGAAAATTCTATATCCATAA
ATAAACGGAGATATAGATGTAATAGGTGCGGACAAACTGATCAGCGGTATTTTTCTTTTTATCACTCATCTGGAAAGAAT
AAGCTGTATTGCCGTTCCTGTGTCATGATGGGCAGAGTGAGTGAGGAGGTTCCTTTATATTCATGGAAAGAGGAAAATGA
ATCAAACTGGAAGTCTATTAAGCTGACATGGGATGGCAAGCTTTCAAGCGGACAGCAAAAAGCCGCCAATGTTTTAATTG
AAGCAATATCAAAAAAAGAAGAGCTCCTCATCTGGGCGGTTTGCGGCGCTGGCAAAACAGAAATGCTGTTTCCTGGTATA
GAATCAGCGTTAAATCAAGGACTGCGTGTATGTATTGCAACACCTCGCACCGATGTTGTATTAGAGCTTGCTCCAAGACT
CAAGGCTGCCTTTCAGGGTGCTGACATTTCAGCGCTTTACGGAGGAAGCGATGACAAAGGGCGGCTATCTCCGCTTATGA
TTTCCACTACGCATCAGCTTTTGCGATATAAAGATGCAATCGATGTTATGATCATTGATGAAGTTGACGCTTTTCCATAT
TCTGCTGATCAAACCCTTCAATTCGCTGTTCAAAAAGCAAGAAAGAAAAACAGCACCCTCGTTTATTTAAGTGCAACACC
TCCTAAAGAATTAAAAAGAAAAGCACTGAACGGACAGTTACATTCAGTTCGCATCCCCGCAAGACACCACCGGAAACCTT
TACCCGAACCGCGCTTTGTATGGTGTGGAAACTGGAAGAAGAAATTAAACCGAAATAAAATTCCGCCAGCGGTGAAAAGA
TGGATAGAGTTTCATGTAAAAGAAGGGAGGCCTGTTTTTTTATTCGTTCCTTCCGTTTCTATTCTGGAAAAGGCTGCTGC
GTGCTTTAAAGGTGTTCATTGCCGAACCGCATCTGTGCACGCGGAAGACAAGCATAGAAAGGAGAAAGTGCAGCAATTCA
GAGATGGTCAGCTCGATCTATTAATCACAACAACAATACTGGAAAGAGGCGTCACAGTCCCCAAGGTGCAAACGGGTGTA
CTAGGAGCGGAATCGTCTATCTTTACGGAAAGCGCACTTGTTCAAATTGCAGGAAGAACCGGCCGGCATAAAGAATATGC
GGACGGCGATGTCATTTACTTTCACTTCGGCAAAACAAAGAGTATGCTCGATGCAAGAAAGCATATAAAAGAAAAGAATG
AATTGGCAGCAAAAGTTGAATGTACAGACTAG

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(120-268)

Helicase conserved C-terminal domain (Helicase_C)

(326-423)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

99.784

100

0.998