Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   PUW21_RS01980 Genome accession   NZ_CP118167
Coordinates   371813..372262 (-) Length   149 a.a.
NCBI ID   WP_003234608.1    Uniprot ID   A0A8E0S750
Organism   Bacillus subtilis strain 21855     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 366813..377262
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUW21_RS01945 (PUW21_01945) yckA 366964..367644 (-) 681 WP_024572176.1 amino acid ABC transporter permease -
  PUW21_RS01950 (PUW21_01950) yckB 367654..368517 (-) 864 WP_029727074.1 transporter substrate-binding domain-containing protein -
  PUW21_RS01955 (PUW21_01955) - 368678..368875 (+) 198 WP_128992271.1 hypothetical protein -
  PUW21_RS01960 (PUW21_01960) yckC 368895..369350 (+) 456 WP_048654748.1 RDD family protein -
  PUW21_RS01965 (PUW21_01965) yckD 369432..369764 (+) 333 WP_015252849.1 YckD family protein -
  PUW21_RS01970 (PUW21_01970) bglC 369918..371351 (+) 1434 WP_048654749.1 6-phospho-beta-glucosidase -
  PUW21_RS01975 (PUW21_01975) nin/comJ 371388..371786 (-) 399 WP_015715226.1 DNA-entry nuclease inhibitor Regulator
  PUW21_RS01980 (PUW21_01980) nucA/comI 371813..372262 (-) 450 WP_003234608.1 DNA-entry nuclease Machinery gene
  PUW21_RS01985 (PUW21_01985) tlpC 372430..374151 (-) 1722 WP_048654750.1 methyl-accepting chemotaxis protein TlpC -
  PUW21_RS01990 (PUW21_01990) hxlB 374262..374819 (-) 558 WP_235120625.1 6-phospho-3-hexuloisomerase -
  PUW21_RS01995 (PUW21_01995) hxlA 374825..375457 (-) 633 WP_003246452.1 3-hexulose-6-phosphate synthase -
  PUW21_RS02000 (PUW21_02000) hxlR 375689..376051 (+) 363 WP_235120624.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16470.42 Da        Isoelectric Point: 4.5282

>NTDB_id=790935 PUW21_RS01980 WP_003234608.1 371813..372262(-) (nucA/comI) [Bacillus subtilis strain 21855]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSTDQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=790935 PUW21_RS01980 WP_003234608.1 371813..372262(-) (nucA/comI) [Bacillus subtilis strain 21855]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGTCTGATAAA
AGGAGACTTTTTTTCAACTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGACACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

97.987

100

0.98