NTDB

Overview

Name	pilD	Type	Machinery gene
Locus tag	PUP58_RS27690	Genome accession	NZ_CP118148
Coordinates	6142390..6143262 (+)	Length	290 a.a.
NCBI ID	WP_009050909.1	Uniprot ID	A0AB33WWI2
Organism	Pseudomonas chlororaphis strain NCCB 880622
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 6137390..6148262

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PUP58_RS27675 (PUP58_27675)	-	6139745..6140101 (-)	357	WP_009050906.1	BON domain-containing protein	-
PUP58_RS27680 (PUP58_27680)	-	6140389..6140805 (-)	417	WP_009050907.1	pilin	-
PUP58_RS27685 (PUP58_27685)	pilC	6141170..6142387 (+)	1218	WP_041987279.1	type II secretion system F family protein	Machinery gene
PUP58_RS27690 (PUP58_27690)	pilD	6142390..6143262 (+)	873	WP_009050909.1	prepilin peptidase	Machinery gene
PUP58_RS27695 (PUP58_27695)	coaE	6143259..6143882 (+)	624	WP_009045669.1	dephospho-CoA kinase	-
PUP58_RS27700 (PUP58_27700)	yacG	6143879..6144082 (+)	204	WP_009045670.1	DNA gyrase inhibitor YacG	-
PUP58_RS27705 (PUP58_27705)	-	6144087..6144302 (-)	216	WP_009050911.1	hypothetical protein	-
PUP58_RS27710 (PUP58_27710)	-	6144368..6145057 (-)	690	WP_009050912.1	energy-coupling factor ABC transporter permease	-
PUP58_RS27715 (PUP58_27715)	-	6145054..6145521 (-)	468	WP_016703672.1	hypothetical protein	-
PUP58_RS27720 (PUP58_27720)	-	6145977..6147413 (+)	1437	WP_009050914.1	undecaprenyl-phosphate glucose phosphotransferase	-

Sequence

Protein

Download Length: 290 a.a. Molecular weight: 32050.22 Da Isoelectric Point: 7.9954

                                >NTDB_id=790632 PUP58_RS27690 WP_009050909.1 6142390..6143262(+) (pilD) [Pseudomonas chlororaphis strain NCCB 880622]

MSLTEVLASYPLVFVASMLVLGLIVGSFLNVLIWRLPKMLERDWHAQARDILGLPVEPAGPTYNLLLPHSQCPHCAHSIR

PWENIPVFSYLLLRGRCSACQAPIGRRYPLVELTCGLASAFVAWHFGFGWQAVLMLLLSWGLLAMSLIDADHQLLPDVLV

LPLLWLGLIVNGFELFVSLHDALWGAVAGYLCLWSVYWLFKLLTGKEGMGHGDFKLLAMFGAWGGWQILPMTLLLASLTG

ALAGIALLRRNRAQMSTPIPFGPFLAIAGWIALLWGGQITGSYLQFVGFR

Nucleotide

Download Length: 873 bp

                                >NTDB_id=790632 PUP58_RS27690 WP_009050909.1 6142390..6143262(+) (pilD) [Pseudomonas chlororaphis strain NCCB 880622]

ATGTCATTGACTGAGGTCCTGGCCAGCTACCCGCTGGTCTTCGTAGCATCGATGCTGGTCCTGGGCCTGATTGTCGGCAG

CTTTCTCAATGTGCTGATCTGGCGTCTGCCAAAGATGCTCGAGCGCGACTGGCACGCCCAGGCCCGTGACATCCTGGGGC

TGCCCGTCGAACCTGCCGGGCCGACCTACAACCTGCTGCTCCCGCATTCCCAGTGCCCTCACTGCGCACATTCGATCCGC

CCCTGGGAAAACATCCCGGTGTTCAGCTACCTGCTGCTGCGCGGACGCTGCTCCGCGTGCCAGGCACCGATCGGCCGGCG

CTACCCGCTGGTGGAGCTGACCTGCGGCCTGGCCTCGGCGTTCGTGGCCTGGCATTTCGGCTTTGGCTGGCAGGCCGTCC

TGATGCTGCTGTTGAGCTGGGGCCTGTTGGCGATGAGCCTGATCGATGCCGATCATCAGCTGTTGCCCGACGTGCTGGTG

CTGCCGCTGTTGTGGCTGGGCCTGATCGTCAATGGCTTCGAGCTGTTCGTCAGCCTCCACGATGCGCTGTGGGGCGCCGT

GGCCGGCTACCTGTGCCTGTGGTCGGTTTACTGGTTGTTCAAGCTGCTGACCGGCAAGGAAGGCATGGGGCACGGTGATT

TCAAGCTGTTGGCCATGTTCGGCGCCTGGGGCGGCTGGCAGATTCTGCCGATGACCCTGCTGCTGGCCTCACTGACGGGA

GCCCTGGCGGGGATCGCCCTGCTGCGCCGCAACCGTGCGCAAATGTCCACGCCTATCCCTTTCGGTCCCTTTCTGGCCAT

TGCCGGCTGGATTGCCTTGCTCTGGGGTGGTCAAATAACCGGCTCCTATTTGCAGTTTGTCGGTTTCAGATGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(137-245)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-126)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilD	Vibrio cholerae strain A1552	52.817	97.931	0.517
pilD	Vibrio campbellii strain DS40M4	52.65	97.586	0.514
pilD	Acinetobacter baumannii D1279779	50.909	94.828	0.483
pilD	Acinetobacter nosocomialis M2	50.182	94.828	0.476
pilD	Neisseria gonorrhoeae MS11	47.794	93.793	0.448

Detailed information NTG0790632